A8GUV9 (A8GUV9_RICB8) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
EC=2.1.1.228 HAMAP MF_00605

Alternative name(s):
M1G-methyltransferase HAMAP MF_00605
tRNA [GM37] methyltransferase HAMAP MF_00605

Gene names

Name:	trmD HAMAP MF_00605 EMBL ABV78626.1
Ordered Locus Names:	A1I_01150

Organism

Rickettsia bellii (strain OSU 85-389) [Complete proteome] [HAMAP] EMBL ABV78626.1

Taxonomic identifier

391896 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm By similarity HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. HAMAP MF_00605

Ontologies

Keywords
Biological process	tRNA processing HAMAP MF_00605
Cellular component	Cytoplasm HAMAP MF_00605
Ligand	S-adenosyl-L-methionine HAMAP MF_00605
Molecular function	Methyltransferase HAMAP MF_00605 EMBL ABV78626.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	163 – 168	6	S-adenosyl-L-methionine binding By similarity HAMAP MF_00605
Sites
	Binding site	143	1	S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP MF_00605

Sequences

	Sequence		Length	Mass (Da)
	A8GUV9 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 666200A31CA91BB1	FASTA	268	30,400
	10 20 30 40 50 60 MFPGTLGHSL AGQALQKNIW SYDVINIRDF GLTKHKNVDD EAYGGGDGLI MRPDVLGNAI 70 80 90 100 110 120 DHALSLNPGA KIYYPSPRGK VFTQGFAKEI LRDIQLNSES FRQDEFKEEP AEHIIIREQR 130 140 150 160 170 180 RMPQNSLVSS LLNDAVNMIF LCGRYEGIDE RVIEEYNVTE ISVGDYILSG GEIPTLTILD 190 200 210 220 230 240 CLIRLLPGVL MNQNTLASES FEKDGEFEGG LECGLYTRPE VWYNRKVPSV LLSGNHKLIN 250 260 EWKKEQSLMI TKLRRPELLK DLRDKERS

References

[1]	"Complete genome sequencing of Rickettsia bellii." Madan A., Lee H., Madan A., Yoon J.-G., Ryu G.-Y., Dasch G., Ereemeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: OSU 85-389 EMBL ABV78626.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000849 Genomic DNA. Translation: ABV78626.1.
RefSeq	YP_001495663.1. NC_009883.1.
3D structure databases
ProteinModelPortal	A8GUV9.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GUV9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5646246.
GenomeReviews	Gene locus A1I_01150 in contig CP000849_GR.
KEGG	rbo:A1I_01150.
PATRIC	17878314. VBIRicBel35792_0240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0336.
HOGENOM	HBG285805.
OMA	QNTLASE.
ProtClustDB	PRK00026.
Enzyme and pathway databases
BioCyc	RBEL391896:A1I_01150-MONOMER.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR022437. RPE3. IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 2 hits. [Graphical view]
TIGRFAMs	TIGR03775. RPE3. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A8GUV9_RICB8

Accession

Primary (citable) accession number: A8GUV9

Entry history

Integrated into UniProtKB/TrEMBL:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	December 14, 2011
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)