SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A8GUH3

- LIPA_RICB8

UniProt

A8GUH3 - LIPA_RICB8

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Lipoyl synthase
Gene
lipA, A1I_00315
Organism
Rickettsia bellii (strain OSU 85-389)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi39 – 391Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi45 – 451Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi60 – 601Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi64 – 641Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRBEL391896:GH75-58-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:A1I_00315
OrganismiRickettsia bellii (strain OSU 85-389)
Taxonomic identifieri391896 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiabelli group
ProteomesiUP000001152: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 303303Lipoyl synthaseUniRule annotation
PRO_0000325304Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi391896.A1I_00315.

Structurei

3D structure databases

ProteinModelPortaliA8GUH3.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0181.
HOGENOMiHOG000235997.
KOiK03644.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

A8GUH3-1 [UniParc]FASTAAdd to Basket

« Hide

MSKRPDWIKV KAPNSSEYYN TKDLIKNLKL NTVCEEAACP NIGECWSKKH    50
ATVMILGSVC TRACRFCNVK TGRPDLLDPH EPQRLAEAVQ KLGLKHVVIT 100
SVDRDDLEDG GATHFAECIS EIRKSSPNTT IEILTPDFLR KDGAAEIIAN 150
AKPDVFNHNV ETVPSLYNTI RPGARYYNSL SLLHNIKKLS PEVFTKSGMM 200
VGLGEEISEV VQVMDDLREA KVDFLTIGQY LQPTKNHAEV AKYVTPEEFK 250
YLERVARTKG FLMVSASPLT RSSYHADEDF EKLKENYRHR HCEERRSIDV 300
AIS 303
Length:303
Mass (Da):34,256
Last modified:March 18, 2008 - v2
Checksum:i5C3C233BCDC83CE1
GO

Sequence cautioni

The sequence ABV78469.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000849 Genomic DNA. Translation: ABV78469.1. Different initiation.
RefSeqiYP_001495506.1. NC_009883.1.

Genome annotation databases

EnsemblBacteriaiABV78469; ABV78469; A1I_00315.
GeneIDi5646460.
KEGGirbo:A1I_00315.
PATRICi17877965. VBIRicBel35792_0067.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000849 Genomic DNA. Translation: ABV78469.1 . Different initiation.
RefSeqi YP_001495506.1. NC_009883.1.

3D structure databases

ProteinModelPortali A8GUH3.
ModBasei Search...

Protein-protein interaction databases

STRINGi 391896.A1I_00315.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV78469 ; ABV78469 ; A1I_00315 .
GeneIDi 5646460.
KEGGi rbo:A1I_00315.
PATRICi 17877965. VBIRicBel35792_0067.

Phylogenomic databases

eggNOGi COG0181.
HOGENOMi HOG000235997.
KOi K03644.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci RBEL391896:GH75-58-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequencing of Rickettsia bellii."
    Madan A., Lee H., Madan A., Yoon J.-G., Ryu G.-Y., Dasch G., Ereemeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: OSU 85-389.

Entry informationi

Entry nameiLIPA_RICB8
AccessioniPrimary (citable) accession number: A8GUH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: March 18, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi