A8GS25 (DAPA_RICRS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	A1G_03345

Organism

Rickettsia rickettsii (strain Sheila Smith) [Complete proteome] [HAMAP]

Taxonomic identifier

392021 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050258
Sites
	Active site	163	1	Schiff-base intermediate with substrate By similarity
	Site	135	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GS25 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: F8198780E49C73C3	FASTA	294	32,490
	10 20 30 40 50 60 MHNIFKGLIT ALITPFKDNK LDLYALERIV KHQIKHEVDA ILIAGSTGES SSLSFEEYKL 70 80 90 100 110 120 LLQTSVEIVN KCIPIISGCS SNNTTYARAL AAESTKIGVD GFMASPPSYV KPTQHGIYKH 130 140 150 160 170 180 FEALHEACNL PIMLYSAPTR SGVDFSDETI LRLSKLPRIL ALKDCGVDLE RPLRIRATVK 190 200 210 220 230 240 KDFNILTGND EVVLAFNAQG GVGWTSVASN IVPNICKELL EKWNKNDTKG ALEIHQKLLP 250 260 270 280 290 LYTALFVESN PIPIKYAAHY LGLCENEIRP PLTEASDSAK KQIENIITSL SIKI

References

[1]	"Complete genome sequence of Rickettsia rickettsii." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sheila Smith.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000848 Genomic DNA. Translation: ABV76200.1.
RefSeq	YP_001494708.1. NC_009882.1.
3D structure databases
ProteinModelPortal	A8GS25.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GS25.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5648526.
GenomeReviews	Gene locus A1G_03345 in contig CP000848_GR.
KEGG	rri:A1G_03345.
PATRIC	17904095. VBIRicRic5337_0709.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	NITATPY.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	RRIC392021:A1G_03345-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_RICRS

Accession

Primary (citable) accession number: A8GS25

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program