A8GR58 (A8GR58_RICRS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP-Rule MF_01897
EC=5.99.1.3 HAMAP-Rule MF_01897

Gene names

Name:	gyrA HAMAP-Rule MF_01897
Ordered Locus Names:	A1G_01555

Organism

Rickettsia rickettsii (strain Sheila Smith) [Complete proteome] [HAMAP] EMBL ABV75883.1

Taxonomic identifier

392021 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group › Rickettsia rickettsii

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. HAMAP-Rule MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP-Rule MF_01897 SAAS SAAS005743
Subunit structure	Heterotetramer, composed of two GyrA and two GyrB chains. Within the heterotetramer, GyrA contains the active site tyrosine that forms a covalent intermediate with the DNA, while GyrB contributes the cofactor binding sites and catalyzes ATP hydrolysis By similarity. HAMAP-Rule MF_01897
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP-Rule MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP-Rule MF_01897
Ligand	ATP-binding HAMAP-Rule MF_01897 SAAS SAAS013760 DNA-binding HAMAP-Rule MF_01897 SAAS SAAS005743 Nucleotide-binding
Molecular function	Isomerase Topoisomerase HAMAP-Rule MF_01897 SAAS SAAS005743
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular_component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase type II (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity HAMAP-Rule MF_01897

Sequences

	Sequence		Length	Mass (Da)
	A8GR58 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 2102A9D94F406604	FASTA	905	100,777
	10 20 30 40 50 60 MTDKSSSNLV PVNIEDEMKV SYLDYAMSVI VSRAIPDVRD GLKPVHRRII YSMHEAGNHA 70 80 90 100 110 120 NKPYRKSARI VGDVMGKYHP HGDSAIYDAL VRMAQDFSLR LPLVDGQGNF GSMDGDAAAA 130 140 150 160 170 180 MRYTESRMAK VAYKLVEDID KETVSFNPNY DGSEEEPSVL PAMFPNLLVN GSGGIAVGMA 190 200 210 220 230 240 TNIPPHNLGE VIDACCLYID NNDIEILDLL EVVKGPDFPT GSMILGISGI RSAYLTGRGS 250 260 270 280 290 300 IIIRGRAEIE NIGNSRQAII ITEIPYMVNK ARLVEKIAQM VKEKRIEGIS DLRDESNKNG 310 320 330 340 350 360 VRIFIELKKD VVAEVVLNQI YACTQLQTSF GVIMLALKDG LPKVMNLKEV IAAFVSFREV 370 380 390 400 410 420 VVTNRTIYLL NKARDRAHIL LGLTIAVSNI DEIIRIIKAS NDPNAAKQEL MARQWEALNI 430 440 450 460 470 480 LPLVKLVDDK AMLNEQGKCN FTEVQAKAIL EMRLQRLTAM EKNKLEEDLK NLTTEITEYL 490 500 510 520 530 540 NILGSRTRLL EILKEELIKV KEEFATPRLT SIEFGEFDQD IEDLIQREEM VVTVTLGGYI 550 560 570 580 590 600 KRVPLSSYRA QKRGGKGRSG LSMRDEDITT QVFVGSTHTP MLFFSNIGQV YSLKLYKLPL 610 620 630 640 650 660 SNPQGKGRPM VNILPLKANE HITNIMPLPE NQDERDNLNI MFATAKGNIR RSDLLDFKKL 670 680 690 700 710 720 QSNGKIAIRL DEDDKLIDVK PCKEDEHILL ATKAGKALRF PVESLRVIKS RTSDGVRGMK 730 740 750 760 770 780 LAKEDSVISM TVLKGISSTK EDRDAYLSVP WEQRLEIAKG EEFNPEELGV SLTAPAIVEM 790 800 810 820 830 840 ANSEEFILTV TENGFGKRSS AYGYRITDRG GSGIINMDIN DKTGLVVGVM PVKMDDELML 850 860 870 880 890 900 ITNSGKLIRC KLESIRITGR NTSGVILFKL DDGEKVVSVS LIAETAEREE DSELEEEGLE QSEDV

References

[1]	"Complete genome sequence of Rickettsia rickettsii." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sheila Smith.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000848 Genomic DNA. Translation: ABV75883.1.
RefSeq	YP_001494391.1. NC_009882.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	392021.A1G_01555.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV75883; ABV75883; A1G_01555.
GeneID	5648497.
KEGG	rri:A1G_01555.
PATRIC	17903311. VBIRicRic5337_0323.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0188.
HOGENOM	HOG000076278.
KO	K02469.
OMA	IAQEDVV.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	RRIC392021:GIY1-293-MONOMER.
Family and domain databases
Gene3D	1.10.268.10. 1 hit. 3.30.1360.40. 1 hit. 3.90.199.10. 1 hit.
HAMAP	MF_01897. GyrA.
InterPro	IPR024946. Arg_repress_C-like. IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_like_dom. [Graphical view]
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A8GR58_RICRS

Accession

Primary (citable) accession number: A8GR58

Entry history

Integrated into UniProtKB/TrEMBL:	November 13, 2007
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)