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A8GR21

- DNAJ_RICRS

UniProt

A8GR21 - DNAJ_RICRS

Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Rickettsia rickettsii (strain Sheila Smith)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

    Cofactori

    Binds 2 zinc ions per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi149 – 1491Zinc 1UniRule annotation
    Metal bindingi152 – 1521Zinc 1UniRule annotation
    Metal bindingi166 – 1661Zinc 2UniRule annotation
    Metal bindingi169 – 1691Zinc 2UniRule annotation
    Metal bindingi188 – 1881Zinc 2UniRule annotation
    Metal bindingi191 – 1911Zinc 2UniRule annotation
    Metal bindingi202 – 2021Zinc 1UniRule annotation
    Metal bindingi205 – 2051Zinc 1UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri136 – 21479CR-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: InterPro
    2. nucleic acid binding Source: InterPro
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA replication Source: UniProtKB-KW
    2. protein folding Source: InterPro
    3. response to heat Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Biological processi

    DNA replication, Stress response

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciRRIC392021:GIY1-256-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chaperone protein DnaJUniRule annotation
    Gene namesi
    Name:dnaJUniRule annotation
    Ordered Locus Names:A1G_01330
    OrganismiRickettsia rickettsii (strain Sheila Smith)
    Taxonomic identifieri392021 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000006832: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 373373Chaperone protein DnaJPRO_1000085276Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi392021.A1G_01330.

    Structurei

    3D structure databases

    ProteinModelPortaliA8GR21.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 6865JUniRule annotationAdd
    BLAST
    Repeati149 – 1568CXXCXGXG motif
    Repeati166 – 1738CXXCXGXG motif
    Repeati188 – 1958CXXCXGXG motif
    Repeati202 – 2098CXXCXGXG motif

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi75 – 11945Gly-richAdd
    BLAST

    Domaini

    The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

    Sequence similaritiesi

    Belongs to the DnaJ family.UniRule annotation
    Contains 1 CR-type zinc finger.UniRule annotation
    Contains 1 J domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri136 – 21479CR-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0484.
    HOGENOMiHOG000226717.
    KOiK03686.
    OMAiVEWPETL.
    OrthoDBiEOG6BPDKP.

    Family and domain databases

    Gene3Di1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPiMF_01152. DnaJ.
    InterProiIPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    IPR001878. Znf_CCHC.
    [Graphical view]
    PfamiPF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view]
    PRINTSiPR00625. JDOMAIN.
    SMARTiSM00271. DnaJ. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view]
    SUPFAMiSSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
    PROSITEiPS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8GR21-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQNYYQILG VSKTASQADL KKAYLKLAKQ YHPDTTDAKD AEKKFKAINA    50
    AYDVLKDEQK RAAYDRLGHD AFQNQQSRGG GGNHGGFHPD INDIFGDFFS 100
    DFMGGSRRSS RPTSAKVRGS DLKYNLTINL EEAFHGIEKN INFSSAVKCN 150
    TCHGSGSEKG ETVTTCDACS GVGATRMQQG FFTIEQACHK CQGNGHIIKN 200
    PCKKCHGMGR YHKQRNLSVN IPAGVENGTR IRHTGEGEAG IRGGNSGDLY 250
    VDITIKPHDI YKVDGANLHC KLPISFVNAA LGGEIEVPVI EGGKVSLTIP 300
    AGTQNGDQLR LRSKGMSKMR STIRGDMLTH IHVEVPKNLS KRQRELLEEF 350
    KKESINEKEN DGSFFNKMKS LWS 373
    Length:373
    Mass (Da):41,072
    Last modified:November 13, 2007 - v1
    Checksum:iFD41F292F984A393
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000848 Genomic DNA. Translation: ABV75846.1.
    RefSeqiWP_012150451.1. NC_009882.1.
    YP_001494354.1. NC_009882.1.

    Genome annotation databases

    EnsemblBacteriaiABV75846; ABV75846; A1G_01330.
    GeneIDi5647829.
    KEGGirri:A1G_01330.
    PATRICi17903223. VBIRicRic5337_0279.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000848 Genomic DNA. Translation: ABV75846.1 .
    RefSeqi WP_012150451.1. NC_009882.1.
    YP_001494354.1. NC_009882.1.

    3D structure databases

    ProteinModelPortali A8GR21.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 392021.A1G_01330.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV75846 ; ABV75846 ; A1G_01330 .
    GeneIDi 5647829.
    KEGGi rri:A1G_01330.
    PATRICi 17903223. VBIRicRic5337_0279.

    Phylogenomic databases

    eggNOGi COG0484.
    HOGENOMi HOG000226717.
    KOi K03686.
    OMAi VEWPETL.
    OrthoDBi EOG6BPDKP.

    Enzyme and pathway databases

    BioCyci RRIC392021:GIY1-256-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.110. 1 hit.
    2.10.230.10. 1 hit.
    HAMAPi MF_01152. DnaJ.
    InterProi IPR012724. DnaJ.
    IPR002939. DnaJ_C.
    IPR001623. DnaJ_domain.
    IPR018253. DnaJ_domain_CS.
    IPR008971. HSP40/DnaJ_pept-bd.
    IPR001305. HSP_DnaJ_Cys-rich_dom.
    IPR001878. Znf_CCHC.
    [Graphical view ]
    Pfami PF01556. CTDII. 1 hit.
    PF00226. DnaJ. 1 hit.
    PF00684. DnaJ_CXXCXGXG. 1 hit.
    [Graphical view ]
    PRINTSi PR00625. JDOMAIN.
    SMARTi SM00271. DnaJ. 1 hit.
    SM00343. ZnF_C2HC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46565. SSF46565. 1 hit.
    SSF49493. SSF49493. 3 hits.
    SSF57938. SSF57938. 1 hit.
    TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
    PROSITEi PS00636. DNAJ_1. 1 hit.
    PS50076. DNAJ_2. 1 hit.
    PS51188. ZF_CR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Rickettsia rickettsii."
      Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Sheila Smith.

    Entry informationi

    Entry nameiDNAJ_RICRS
    AccessioniPrimary (citable) accession number: A8GR21
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3