Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A8GR21

- DNAJ_RICRS

UniProt

A8GR21 - DNAJ_RICRS

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Rickettsia rickettsii (strain Sheila Smith)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn(2+) ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi149 – 1491Zinc 1UniRule annotation
Metal bindingi152 – 1521Zinc 1UniRule annotation
Metal bindingi166 – 1661Zinc 2UniRule annotation
Metal bindingi169 – 1691Zinc 2UniRule annotation
Metal bindingi188 – 1881Zinc 2UniRule annotation
Metal bindingi191 – 1911Zinc 2UniRule annotation
Metal bindingi202 – 2021Zinc 1UniRule annotation
Metal bindingi205 – 2051Zinc 1UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri136 – 21479CR-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. nucleic acid binding Source: InterPro
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein folding Source: InterPro
  3. response to heat Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciRRIC392021:GIY1-256-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:A1G_01330
OrganismiRickettsia rickettsii (strain Sheila Smith)
Taxonomic identifieri392021 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000006832: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Chaperone protein DnaJPRO_1000085276Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi392021.A1G_01330.

Structurei

3D structure databases

ProteinModelPortaliA8GR21.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 6865JUniRule annotationAdd
BLAST
Repeati149 – 1568CXXCXGXG motif
Repeati166 – 1738CXXCXGXG motif
Repeati188 – 1958CXXCXGXG motif
Repeati202 – 2098CXXCXGXG motif

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 11945Gly-richAdd
BLAST

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation
Contains 1 CR-type zinc finger.UniRule annotation
Contains 1 J domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri136 – 21479CR-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
HOGENOMiHOG000226717.
KOiK03686.
OMAiVEWPETL.
OrthoDBiEOG6BPDKP.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GR21-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQNYYQILG VSKTASQADL KKAYLKLAKQ YHPDTTDAKD AEKKFKAINA
60 70 80 90 100
AYDVLKDEQK RAAYDRLGHD AFQNQQSRGG GGNHGGFHPD INDIFGDFFS
110 120 130 140 150
DFMGGSRRSS RPTSAKVRGS DLKYNLTINL EEAFHGIEKN INFSSAVKCN
160 170 180 190 200
TCHGSGSEKG ETVTTCDACS GVGATRMQQG FFTIEQACHK CQGNGHIIKN
210 220 230 240 250
PCKKCHGMGR YHKQRNLSVN IPAGVENGTR IRHTGEGEAG IRGGNSGDLY
260 270 280 290 300
VDITIKPHDI YKVDGANLHC KLPISFVNAA LGGEIEVPVI EGGKVSLTIP
310 320 330 340 350
AGTQNGDQLR LRSKGMSKMR STIRGDMLTH IHVEVPKNLS KRQRELLEEF
360 370
KKESINEKEN DGSFFNKMKS LWS
Length:373
Mass (Da):41,072
Last modified:November 13, 2007 - v1
Checksum:iFD41F292F984A393
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000848 Genomic DNA. Translation: ABV75846.1.
RefSeqiWP_012150451.1. NC_009882.1.
YP_001494354.1. NC_009882.1.

Genome annotation databases

EnsemblBacteriaiABV75846; ABV75846; A1G_01330.
GeneIDi5647829.
KEGGirri:A1G_01330.
PATRICi17903223. VBIRicRic5337_0279.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000848 Genomic DNA. Translation: ABV75846.1 .
RefSeqi WP_012150451.1. NC_009882.1.
YP_001494354.1. NC_009882.1.

3D structure databases

ProteinModelPortali A8GR21.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 392021.A1G_01330.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV75846 ; ABV75846 ; A1G_01330 .
GeneIDi 5647829.
KEGGi rri:A1G_01330.
PATRICi 17903223. VBIRicRic5337_0279.

Phylogenomic databases

eggNOGi COG0484.
HOGENOMi HOG000226717.
KOi K03686.
OMAi VEWPETL.
OrthoDBi EOG6BPDKP.

Enzyme and pathway databases

BioCyci RRIC392021:GIY1-256-MONOMER.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPi MF_01152. DnaJ.
InterProi IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Rickettsia rickettsii."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sheila Smith.

Entry informationi

Entry nameiDNAJ_RICRS
AccessioniPrimary (citable) accession number: A8GR21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3