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A8GR21

- DNAJ_RICRS

UniProt

A8GR21 - DNAJ_RICRS

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Protein

Chaperone protein DnaJ

Gene
dnaJ, A1G_01330
Organism
Rickettsia rickettsii (strain Sheila Smith)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins By similarity.UniRule annotation

Cofactori

Binds 2 zinc ions per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi149 – 1491Zinc 1 By similarity
Metal bindingi152 – 1521Zinc 1 By similarity
Metal bindingi166 – 1661Zinc 2 By similarity
Metal bindingi169 – 1691Zinc 2 By similarity
Metal bindingi188 – 1881Zinc 2 By similarity
Metal bindingi191 – 1911Zinc 2 By similarity
Metal bindingi202 – 2021Zinc 1 By similarity
Metal bindingi205 – 2051Zinc 1 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri136 – 21479CR-typeUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: InterPro
  2. nucleic acid binding Source: InterPro
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA replication Source: UniProtKB-KW
  2. protein folding Source: InterPro
  3. response to heat Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

DNA replication, Stress response

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciRRIC392021:GIY1-256-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJ
Gene namesi
Name:dnaJ
Ordered Locus Names:A1G_01330
OrganismiRickettsia rickettsii (strain Sheila Smith)
Taxonomic identifieri392021 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000006832: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 373373Chaperone protein DnaJUniRule annotationPRO_1000085276Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi392021.A1G_01330.

Structurei

3D structure databases

ProteinModelPortaliA8GR21.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 6865JAdd
BLAST
Repeati149 – 1568CXXCXGXG motifUniRule annotation
Repeati166 – 1738CXXCXGXG motifUniRule annotation
Repeati188 – 1958CXXCXGXG motifUniRule annotation
Repeati202 – 2098CXXCXGXG motifUniRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi75 – 11945Gly-richUniRule annotationAdd
BLAST

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.
Contains 1 J domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri136 – 21479CR-typeUniRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0484.
HOGENOMiHOG000226717.
KOiK03686.
OMAiVEWPETL.
OrthoDBiEOG6BPDKP.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR001878. Znf_CCHC.
[Graphical view]
PfamiPF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GR21-1 [UniParc]FASTAAdd to Basket

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MSQNYYQILG VSKTASQADL KKAYLKLAKQ YHPDTTDAKD AEKKFKAINA    50
AYDVLKDEQK RAAYDRLGHD AFQNQQSRGG GGNHGGFHPD INDIFGDFFS 100
DFMGGSRRSS RPTSAKVRGS DLKYNLTINL EEAFHGIEKN INFSSAVKCN 150
TCHGSGSEKG ETVTTCDACS GVGATRMQQG FFTIEQACHK CQGNGHIIKN 200
PCKKCHGMGR YHKQRNLSVN IPAGVENGTR IRHTGEGEAG IRGGNSGDLY 250
VDITIKPHDI YKVDGANLHC KLPISFVNAA LGGEIEVPVI EGGKVSLTIP 300
AGTQNGDQLR LRSKGMSKMR STIRGDMLTH IHVEVPKNLS KRQRELLEEF 350
KKESINEKEN DGSFFNKMKS LWS 373
Length:373
Mass (Da):41,072
Last modified:November 13, 2007 - v1
Checksum:iFD41F292F984A393
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000848 Genomic DNA. Translation: ABV75846.1.
RefSeqiWP_012150451.1. NC_009882.1.
YP_001494354.1. NC_009882.1.

Genome annotation databases

EnsemblBacteriaiABV75846; ABV75846; A1G_01330.
GeneIDi5647829.
KEGGirri:A1G_01330.
PATRICi17903223. VBIRicRic5337_0279.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000848 Genomic DNA. Translation: ABV75846.1 .
RefSeqi WP_012150451.1. NC_009882.1.
YP_001494354.1. NC_009882.1.

3D structure databases

ProteinModelPortali A8GR21.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 392021.A1G_01330.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV75846 ; ABV75846 ; A1G_01330 .
GeneIDi 5647829.
KEGGi rri:A1G_01330.
PATRICi 17903223. VBIRicRic5337_0279.

Phylogenomic databases

eggNOGi COG0484.
HOGENOMi HOG000226717.
KOi K03686.
OMAi VEWPETL.
OrthoDBi EOG6BPDKP.

Enzyme and pathway databases

BioCyci RRIC392021:GIY1-256-MONOMER.

Family and domain databases

Gene3Di 1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPi MF_01152. DnaJ.
InterProi IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
IPR001878. Znf_CCHC.
[Graphical view ]
Pfami PF01556. CTDII. 1 hit.
PF00226. DnaJ. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
[Graphical view ]
PRINTSi PR00625. JDOMAIN.
SMARTi SM00271. DnaJ. 1 hit.
SM00343. ZnF_C2HC. 1 hit.
[Graphical view ]
SUPFAMi SSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsi TIGR02349. DnaJ_bact. 1 hit.
PROSITEi PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Rickettsia rickettsii."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sheila Smith.

Entry informationi

Entry nameiDNAJ_RICRS
AccessioniPrimary (citable) accession number: A8GR21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: September 3, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi