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Protein

Ribonuclease 3

Gene

rnc

Organism
Rickettsia rickettsii (strain Sheila Smith)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Processes some mRNAs, and tRNAs when they are encoded in the rRNA operon. Processes pre-crRNA and tracrRNA of type II CRISPR loci if present in the organism.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461MagnesiumUniRule annotation
Active sitei50 – 501UniRule annotation
Metal bindingi119 – 1191MagnesiumUniRule annotation
Active sitei122 – 1221UniRule annotation
Metal bindingi122 – 1221MagnesiumUniRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. ribonuclease III activity Source: UniProtKB-HAMAP
  3. rRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mRNA processing Source: UniProtKB-HAMAP
  2. rRNA catabolic process Source: InterPro
  3. rRNA processing Source: UniProtKB-HAMAP
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciRRIC392021:GIY1-171-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease 3UniRule annotation (EC:3.1.26.3UniRule annotation)
Alternative name(s):
Ribonuclease IIIUniRule annotation
Short name:
RNase IIIUniRule annotation
Gene namesi
Name:rncUniRule annotation
Ordered Locus Names:A1G_00905
OrganismiRickettsia rickettsii (strain Sheila Smith)
Taxonomic identifieri392021 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000006832: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Ribonuclease 3PRO_0000316270Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi392021.A1G_00905.

Structurei

3D structure databases

ProteinModelPortaliA8GQT8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 133130RNase IIIUniRule annotationAdd
BLAST
Domaini158 – 22669DRBMUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 DRBM (double-stranded RNA-binding) domain.UniRule annotation
Contains 1 RNase III domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246808.
KOiK03685.
OMAiSADHNER.
OrthoDBiEOG6T1WVS.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GQT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESFEKLEKL LSYSFKNKEL LIEALSHPSL RQHHEYKDDK DYERLEFLGD
60 70 80 90 100
AVLNLVITEI LFRNFANYNE GNLAKIRSYL VCKETICMVG AKLTLKNYII
110 120 130 140 150
MTHGEEVAGG RDNLNNIENA TEALIAAIYL DSNIETTHDI IEKLWAEFIK
160 170 180 190 200
VQNLTDYDPK TALQEWAQAS DHHLPIYRLI KREGASHSST FTVLVKVKDY
210 220
EQTGTGHAIK EAEKNAARSL LHRLKND
Length:227
Mass (Da):26,067
Last modified:November 13, 2007 - v1
Checksum:iE7481B1E507843A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000848 Genomic DNA. Translation: ABV75763.1.
AY134668 Genomic DNA. No translation available.
RefSeqiYP_001494271.1. NC_009882.1.

Genome annotation databases

EnsemblBacteriaiABV75763; ABV75763; A1G_00905.
GeneIDi5648030.
KEGGirri:A1G_00905.
PATRICi17903043. VBIRicRic5337_0191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000848 Genomic DNA. Translation: ABV75763.1.
AY134668 Genomic DNA. No translation available.
RefSeqiYP_001494271.1. NC_009882.1.

3D structure databases

ProteinModelPortaliA8GQT8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi392021.A1G_00905.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV75763; ABV75763; A1G_00905.
GeneIDi5648030.
KEGGirri:A1G_00905.
PATRICi17903043. VBIRicRic5337_0191.

Phylogenomic databases

eggNOGiCOG0571.
HOGENOMiHOG000246808.
KOiK03685.
OMAiSADHNER.
OrthoDBiEOG6T1WVS.

Enzyme and pathway databases

BioCyciRRIC392021:GIY1-171-MONOMER.

Family and domain databases

Gene3Di1.10.1520.10. 1 hit.
3.30.160.20. 1 hit.
HAMAPiMF_00104. RNase_III.
InterProiIPR014720. dsRNA-bd_dom.
IPR011907. RNase_III.
IPR000999. RNase_III_dom.
[Graphical view]
PANTHERiPTHR11207. PTHR11207. 1 hit.
PfamiPF00035. dsrm. 1 hit.
PF14622. Ribonucleas_3_3. 1 hit.
[Graphical view]
SMARTiSM00358. DSRM. 1 hit.
SM00535. RIBOc. 1 hit.
[Graphical view]
SUPFAMiSSF69065. SSF69065. 1 hit.
TIGRFAMsiTIGR02191. RNaseIII. 1 hit.
PROSITEiPS50137. DS_RBD. 1 hit.
PS00517. RNASE_3_1. 1 hit.
PS50142. RNASE_3_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Rickettsia rickettsii."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sheila Smith.
  2. "Molecular and functional analysis of the lepB gene, encoding a type I signal peptidase from Rickettsia rickettsii and Rickettsia typhi."
    Rahman M.S., Simser J.A., Macaluso K.R., Azad A.F.
    J. Bacteriol. 185:4578-4584(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-101, TRANSCRIPT ANALYSIS (OPERON STRUCTURE).

Entry informationi

Entry nameiRNC_RICRS
AccessioniPrimary (citable) accession number: A8GQT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: January 7, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Belongs to an operon consisting of at least secF-nuoF-lepB-rnc.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.