Reviewed, UniProtKB/Swiss-Prot A8GQT7 (LEP_RICRS)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	A1G_00900

Organism

Rickettsia rickettsii (strain Sheila Smith) [Complete proteome] [HAMAP]

Taxonomic identifier

392021 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Complements E.coli mutants temperature-sensitive for lepB function.
Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein Potential.
Miscellaneous	Belongs to an operon consisting of at least secF-nuoF-lepB-rnc.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Signal peptidase I		PRO_0000316277
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Potential
	Topological domain	42 – 266	225	Periplasmic Potential
Sites
	Active site	45	1	By similarity
	Active site	108	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GQT7-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: EAA0EC1E1FE91DAA	FASTA	266	31,083
	10 20 30 40 50 60 MQTDNTKSNT NKTAKQEWGS FAFVICIALL IRILIMEPFT VPTGSMKATI LENDYIFSTK 70 80 90 100 110 120 YSYGYSNYSL SFFDFIPLFK GRIFAREPDR GDIVVFRPPN DMSVRYIKRL IGLPGDKIQL 130 140 150 160 170 180 IDDVIYINDK KIERTEVGTY ISEEGIKYLK FKETLPNGRT YFSYKLAPIY GVIYNDRYGN 190 200 210 220 230 240 TDVFYVPEGK YFFLGDNRDQ SNDSRVNLGF VPFENFIAKA QFIWFSTKIT WWDNDIGVIN 250 260 LVLKLKPWVE SVRLNRIFRN LYNTDA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and functional analysis of the lepB gene, encoding a type I signal peptidase from Rickettsia rickettsii and Rickettsia typhi." Rahman M.S., Simser J.A., Macaluso K.R., Azad A.F. J. Bacteriol. 185:4578-4584(2003) [PubMed: 12867468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], OPERON STRUCTURE, CHARACTERIZATION IN E.COLI.
[2]	"Complete genome sequence of Rickettsia rickettsii." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY134668 Genomic DNA. Translation: AAN04256.1. CP000848 Genomic DNA. Translation: ABV75762.1.
RefSeq	YP_001494270.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S26.001.
Genome annotation databases
GeneID	5648029.
GenomeReviews	Gene locus A1G_00900 in contig CP000848_GR.
KEGG	rri:A1G_00900.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8GQT7. KPWIESV.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_RICRS

Accession

Primary (citable) accession number: A8GQT7
Secondary accession number(s): Q8GE73

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)