Reviewed, UniProtKB/Swiss-Prot A8GQT6 (NUOF_RICRS)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit F
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit F
    NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	A1G_00895

Organism

Rickettsia rickettsii (strain Sheila Smith) [Complete proteome] [HAMAP]

Taxonomic identifier

392021 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Miscellaneous	Belongs to an operon consisting of at least secF-nuoF-lepB-rnc.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	NADH-quinone oxidoreductase subunit F		PRO_0000316288
Regions
	Nucleotide binding	54 – 63	10	NAD By similarity
	Nucleotide binding	166 – 213	48	FMN By similarity
Sites
	Metal binding	344	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	347	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	350	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	390	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A8GQT6-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: D57700F4C0755749	FASTA	421	46,102
	10 20 30 40 50 60 MLKEEDKIFT NLHGQQSHDL KSSKKRGDWE NTKALLDKGR DFIVEEVKKS GLRGRGGAGF 70 80 90 100 110 120 STGMKWSFMP KNLEKSCYLV VNADESEPGT CKDRDILRFE PHKLIEGCLL ASFAIGANNC 130 140 150 160 170 180 YIYIRGEFYN EASNIQRALD EAYKEGLIGK NSCGSGFDCN IYLHRGAGAY ICGEETALLE 190 200 210 220 230 240 SLEGKKGMPR LKPPFPAGFG LYGCPTTINN VESIAVVPTI LRRGASWFAG IGKPNNTGTK 250 260 270 280 290 300 IFCISGHVNK PCNVEEAMGI SLKELIEKYA GGVRGGWDNL KAIIPGGSSV PLLPKSLCEV 310 320 330 340 350 360 DMDFDSLRTA GSGLGTGGII VMDQSTDIIY AIARLSKFYM HESCGQCTPC REGTGWMWRV 370 380 390 400 410 420 MMRLVKGNVT KSEIDELLNV TKAIEGHTIC ALGDAAAWPI QGLIRHFRSE IEARIKSYSV V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and functional analysis of the lepB gene, encoding a type I signal peptidase from Rickettsia rickettsii and Rickettsia typhi." Rahman M.S., Simser J.A., Macaluso K.R., Azad A.F. J. Bacteriol. 185:4578-4584(2003) [PubMed: 12867468] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TRANSCRIPT ANALYSIS (OPERON STRUCTURE).
[2]	"Complete genome sequence of Rickettsia rickettsii." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY134668 Genomic DNA. Translation: AAO00970.1. CP000848 Genomic DNA. Translation: ABV75761.1.
RefSeq	YP_001494269.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5648069.
GenomeReviews	Gene locus A1G_00895 in contig CP000848_GR.
KEGG	rri:A1G_00895.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8GQT6. PCNVEEE.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51KDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51KDa_su. IPR019554. Soluble_ligand_bd. [Graphical view]
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. nuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_RICRS

Accession

Primary (citable) accession number: A8GQT6
Secondary accession number(s): Q8GE74

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)