A8GQN6 (QUEF_RICRS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	A1G_00625

Organism

Rickettsia rickettsii (strain Sheila Smith) [Complete proteome] [HAMAP]

Taxonomic identifier

392021 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000062360

Sequences

	Sequence		Length	Mass (Da)
	A8GQN6 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: BCA8B3AE7610F1D4	FASTA	273	31,249
	10 20 30 40 50 60 MPLSTSLLGK KSTYKDSYDV TLLFKIPRIN NRNELGINSN NLPFYGVDVW NTYELSCLNK 70 80 90 100 110 120 NGKPWVGVGT FYIPTDSENI VESKSFKLYL NSFNNFVVES VKELERIILQ DLSNVTHAKV 130 140 150 160 170 180 TGRIFPINTK VEFGVPSGKN IDDLDIVCNN YGAPDNSLIE YEDVLVEEEI NSNLLKSNCL 190 200 210 220 230 240 VTSQPDWGTI VIKYKGKKLK YDSFLKYLIS FRNCNEFAEQ CAERIFTDIQ NAISPDFLSI 250 260 270 YIVYARRGGI DICPYRSTDK NYTLPSDKRF IRQ

References

[1]	"Complete genome sequence of Rickettsia rickettsii." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sheila Smith.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000848 Genomic DNA. Translation: ABV75711.1.
RefSeq	YP_001494219.1. NC_009882.1.
3D structure databases
ProteinModelPortal	A8GQN6.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GQN6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5647850.
GenomeReviews	Gene locus A1G_00625 in contig CP000848_GR.
KEGG	rri:A1G_00625.
PATRIC	17902921. VBIRicRic5337_0132.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG289139.
OMA	CVERIFC.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	RRIC392021:A1G_00625-MONOMER.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_RICRS

Accession

Primary (citable) accession number: A8GQN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program