A8GQD0 (LPXD_RICRS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	A1G_00045

Organism

Rickettsia rickettsii (strain Sheila Smith) [Complete proteome] [HAMAP]

Taxonomic identifier

392021 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000050959
Sites
	Active site	252	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GQD0 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 41577509BD4D9CDF	FASTA	345	36,794
	10 20 30 40 50 60 MVSSNFYKNL GPRKLTAIID FLHDIIAPPK IEDIAIHDIK ILQEASPNDI SFLSNPKYSE 70 80 90 100 110 120 FLKTTKAAAC IVPKNFTGEA NPNTVLLHAQ NSYFAYGKLI DFFYAPIKSY PTKIMKSAIV 130 140 150 160 170 180 ADSATIGKNC YIGHNVVIED DVIIGDNSII EAGSFIGRGV NIGRNARIEQ HVSINYAIIG 190 200 210 220 230 240 DDVVILAGAK IGQDGFGFST EKGVHHKIFH IGIVKIGNNV EIGANTTIDR GSLQDTIIKD 250 260 270 280 290 300 LCRIDNLVQI GHGVKIGKGS IIVAQTGIAG SSTIGKYCAL GGQVGIAGHL NIGDGAQVAA 310 320 330 340 QGGVAQNIEA GKIVGGSPAI PIMDWHRQSI IMKQLLKTSN SKLKK

References

[1]	"Complete genome sequence of Rickettsia rickettsii." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sheila Smith.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000848 Genomic DNA. Translation: ABV75605.1.
RefSeq	YP_001494113.1. NC_009882.1.
3D structure databases
ProteinModelPortal	A8GQD0.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GQD0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5648261.
GenomeReviews	Gene locus A1G_00045 in contig CP000848_GR.
KEGG	rri:A1G_00045.
PATRIC	17902669. VBIRicRic5337_0008.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	SYPAKIM.
ProtClustDB	PRK00892.
Enzyme and pathway databases
BioCyc	RRIC392021:A1G_00045-MONOMER.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 5 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_RICRS

Accession

Primary (citable) accession number: A8GQD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program