A8GPB2 (SYE2_RICAH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	A1C_04895

Organism

Rickettsia akari (strain Hartford) [Complete proteome] [HAMAP]

Taxonomic identifier

293614 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000367753
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	239 – 243	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	242	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GPB2 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: D0053298392C2F34	FASTA	463	52,433
	10 20 30 40 50 60 MTNVITRFAP SPTGFLHIGS ARTALFNYLF ARHNNGKFLL RIEDTDKERS TKAAVDAIFS 70 80 90 100 110 120 GLKWLGLDWD AEVIFQSKRN DLYKKAALRL LKEGKAYYCF TSQEEIEKQR QKALENKQHF 130 140 150 160 170 180 IFNSEWRDKE PDAYPTDVKP VIRLKTPREG RITIHDTLQG EVVIENSHID DMVLLRADGT 190 200 210 220 230 240 ATYMLAVVAD DHDMGITHII RGDDHLTNAA RQIAIYQALG YEVPSMTHIP LIHGADGAKL 250 260 270 280 290 300 SKRHGALGVD AYKNMGYLPE SLCNYLLRLG WSHGNDEIIS MTQAIEWFNL DSLGKSPSRL 310 320 330 340 350 360 DFAKMNSLNA YYLRMLDNDT LTAKTVEILK QNYKISDKEV SYIKQAMSSL LVRSETLLDL 370 380 390 400 410 420 AQLAQIYLVD SPIVYNQDAK EIIENCNKDL IKQVTEGLNK IEKFDKESVQ NKFKEIAAAN 430 440 450 460 DLKLSDIMKP VRALITGMDA SPSVFAIAEI LGKENILKRL ETI

References

[1]	"Complete genome sequence of Rickettsia akari." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hartford.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000847 Genomic DNA. Translation: ABV75237.1.
RefSeq	YP_001493745.1. NC_009881.1.
3D structure databases
ProteinModelPortal	A8GPB2.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GPB2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5645637.
GenomeReviews	Gene locus A1C_04895 in contig CP000847_GR.
KEGG	rak:A1C_04895.
NMPDR	fig|293614.3.peg.915.
PATRIC	17876960. VBIRicAka50705_1015.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	IEWFNLD.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	RAKA293614:A1C_04895-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_RICAH

Accession

Primary (citable) accession number: A8GPB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program