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A8GPA1

- SYI_RICAH

UniProt

A8GPA1 - SYI_RICAH

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia akari (strain Hartford)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei631 – 6311ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRAKA293614:GI4A-913-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:A1C_04840
OrganismiRickettsia akari (strain Hartford)
Taxonomic identifieri293614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000006830: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11031103Isoleucine--tRNA ligasePRO_1000022158Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi293614.A1C_04840.

Structurei

3D structure databases

ProteinModelPortaliA8GPA1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi628 – 6325"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR022439. RPE4.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
TIGR03777. RPE4. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GPA1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANTKYYPEV SSNANFAAIE REILKFWQDN NIFQKSIDGR NGESEFIFYD
60 70 80 90 100
GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ
110 120 130 140 150
SEKELGISGR LAITNFGIEK FNAHCRASVM EYASDWEEYV TRQARWVDFK
160 170 180 190 200
NSYKTMDKNF MESVLWAFKE LYNKGLLCES MRVVPYSWAC ETPLSNFETR
210 220 230 240 250
LDNSYRERSD KAVTVSFVLR DKLHEIPAFA GMISRESEMT VGGDYQEYRI
260 270 280 290 300
LTWTTTPWTL PSNLAIAVGS DIDYALVPQE NICYIIAASS VSKYAKELGL
310 320 330 340 350
SGEENFEIIK GSQLQGLRYK PLFDYFEHHP NSFKIFDVDF VVEGDGTGVV
360 370 380 390 400
HMAPGFGEDD QILCESKGIS LVCPVDNSGK FTKEIPDLEG VQVFDANDKI
410 420 430 440 450
IIKLKEQGNW LKTEQYIHNY PHCWRTDTPL IYKAVPSWYV KVTQFKDRMV
460 470 480 490 500
ELNQQINWIP HHVKDNLFGK WLENARDWSI SRNRFWGTPL PVWKSDDPKY
510 520 530 540 550
PRIDVYGSIE ELEKDFGVKV TDLHRPFIDK LTRPNPNDPT GKSTMRRIED
560 570 580 590 600
VFDCWFESGS MPYGQAHYPF ENKEWFEDHF PADFIVEYSA QTRGWFYTLM
610 620 630 640 650
VLSTALFDRP PFLNCICHGV ILDATGQKLS KRLNNYADPL ELFDQYGSDA
660 670 680 690 700
LRVTMLSSNI VKGQELLIDK DGKMVFDTLR LFIKPIWSSY HFFTMYANAD
710 720 730 740 750
SLKGEISFAS ENVLDVYILS KLKIAVSKIE ESLDNFDTQT AYHAVLEFFE
760 770 780 790 800
VLNNWYIRRS RARFWKSEKD TDKQNAYNTL YSCLKTMAIA MSALVPMISE
810 820 830 840 850
AIYKGLCHCE ETSTLSSRDL IAGSSKSINN LNPVVKPRDY TPSVHHNDQI
860 870 880 890 900
SVHLCNYPTL SDFEINHELV ATMDNVLDIC SNSLFIRSTK NIRVRQPLAS
910 920 930 940 950
ITIISKHNND LKAFENLIKD EINVKSVIYC DDLENYASKK LSINFPMLGK
960 970 980 990 1000
RLPAKMKEII AASKKGDWKA IAGGLTICGE TLNNEEYKLI LEPYSHIKGA
1010 1020 1030 1040 1050
ASFENNSSLL ILDLELTAEL IEEGYARDIV RFIQQARKDA GFSITDRILI
1060 1070 1080 1090 1100
EIISEFDLSE IIYNYGDFIT EQTLGEFSKN FTPDYVSKVE LEDHPIQLKI

KKS
Length:1,103
Mass (Da):126,771
Last modified:November 13, 2007 - v1
Checksum:i2D5D718E96221D88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000847 Genomic DNA. Translation: ABV75226.1.
RefSeqiYP_001493734.1. NC_009881.1.

Genome annotation databases

EnsemblBacteriaiABV75226; ABV75226; A1C_04840.
GeneIDi5645801.
KEGGirak:A1C_04840.
PATRICi17876936. VBIRicAka50705_1003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000847 Genomic DNA. Translation: ABV75226.1 .
RefSeqi YP_001493734.1. NC_009881.1.

3D structure databases

ProteinModelPortali A8GPA1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 293614.A1C_04840.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV75226 ; ABV75226 ; A1C_04840 .
GeneIDi 5645801.
KEGGi rak:A1C_04840.
PATRICi 17876936. VBIRicAka50705_1003.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RAKA293614:GI4A-913-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR022439. RPE4.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
TIGR03777. RPE4. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Rickettsia akari."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hartford.

Entry informationi

Entry nameiSYI_RICAH
AccessioniPrimary (citable) accession number: A8GPA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3