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A8GPA1

- SYI_RICAH

UniProt

A8GPA1 - SYI_RICAH

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Protein

Isoleucine--tRNA ligase

Gene
ileS, A1C_04840
Organism
Rickettsia akari (strain Hartford)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei631 – 6311ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciRAKA293614:GI4A-913-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:A1C_04840
OrganismiRickettsia akari (strain Hartford)
Taxonomic identifieri293614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000006830: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11031103Isoleucine--tRNA ligaseUniRule annotationPRO_1000022158Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi293614.A1C_04840.

Structurei

3D structure databases

ProteinModelPortaliA8GPA1.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionUniRule annotationAdd
BLAST
Motifi628 – 6325"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR022439. RPE4.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
TIGR03777. RPE4. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GPA1-1 [UniParc]FASTAAdd to Basket

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MANTKYYPEV SSNANFAAIE REILKFWQDN NIFQKSIDGR NGESEFIFYD     50
GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ 100
SEKELGISGR LAITNFGIEK FNAHCRASVM EYASDWEEYV TRQARWVDFK 150
NSYKTMDKNF MESVLWAFKE LYNKGLLCES MRVVPYSWAC ETPLSNFETR 200
LDNSYRERSD KAVTVSFVLR DKLHEIPAFA GMISRESEMT VGGDYQEYRI 250
LTWTTTPWTL PSNLAIAVGS DIDYALVPQE NICYIIAASS VSKYAKELGL 300
SGEENFEIIK GSQLQGLRYK PLFDYFEHHP NSFKIFDVDF VVEGDGTGVV 350
HMAPGFGEDD QILCESKGIS LVCPVDNSGK FTKEIPDLEG VQVFDANDKI 400
IIKLKEQGNW LKTEQYIHNY PHCWRTDTPL IYKAVPSWYV KVTQFKDRMV 450
ELNQQINWIP HHVKDNLFGK WLENARDWSI SRNRFWGTPL PVWKSDDPKY 500
PRIDVYGSIE ELEKDFGVKV TDLHRPFIDK LTRPNPNDPT GKSTMRRIED 550
VFDCWFESGS MPYGQAHYPF ENKEWFEDHF PADFIVEYSA QTRGWFYTLM 600
VLSTALFDRP PFLNCICHGV ILDATGQKLS KRLNNYADPL ELFDQYGSDA 650
LRVTMLSSNI VKGQELLIDK DGKMVFDTLR LFIKPIWSSY HFFTMYANAD 700
SLKGEISFAS ENVLDVYILS KLKIAVSKIE ESLDNFDTQT AYHAVLEFFE 750
VLNNWYIRRS RARFWKSEKD TDKQNAYNTL YSCLKTMAIA MSALVPMISE 800
AIYKGLCHCE ETSTLSSRDL IAGSSKSINN LNPVVKPRDY TPSVHHNDQI 850
SVHLCNYPTL SDFEINHELV ATMDNVLDIC SNSLFIRSTK NIRVRQPLAS 900
ITIISKHNND LKAFENLIKD EINVKSVIYC DDLENYASKK LSINFPMLGK 950
RLPAKMKEII AASKKGDWKA IAGGLTICGE TLNNEEYKLI LEPYSHIKGA 1000
ASFENNSSLL ILDLELTAEL IEEGYARDIV RFIQQARKDA GFSITDRILI 1050
EIISEFDLSE IIYNYGDFIT EQTLGEFSKN FTPDYVSKVE LEDHPIQLKI 1100
KKS 1103
Length:1,103
Mass (Da):126,771
Last modified:November 13, 2007 - v1
Checksum:i2D5D718E96221D88
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000847 Genomic DNA. Translation: ABV75226.1.
RefSeqiYP_001493734.1. NC_009881.1.

Genome annotation databases

EnsemblBacteriaiABV75226; ABV75226; A1C_04840.
GeneIDi5645801.
KEGGirak:A1C_04840.
PATRICi17876936. VBIRicAka50705_1003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000847 Genomic DNA. Translation: ABV75226.1 .
RefSeqi YP_001493734.1. NC_009881.1.

3D structure databases

ProteinModelPortali A8GPA1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 293614.A1C_04840.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV75226 ; ABV75226 ; A1C_04840 .
GeneIDi 5645801.
KEGGi rak:A1C_04840.
PATRICi 17876936. VBIRicAka50705_1003.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci RAKA293614:GI4A-913-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 2 hits.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR022439. RPE4.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
TIGR03777. RPE4. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Rickettsia akari."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hartford.

Entry informationi

Entry nameiSYI_RICAH
AccessioniPrimary (citable) accession number: A8GPA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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