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A8GPA1

- SYI_RICAH

UniProt

A8GPA1 - SYI_RICAH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Rickettsia akari (strain Hartford)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei631 – 6311ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciRAKA293614:GI4A-913-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:A1C_04840
    OrganismiRickettsia akari (strain Hartford)
    Taxonomic identifieri293614 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
    ProteomesiUP000006830: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11031103Isoleucine--tRNA ligasePRO_1000022158Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi293614.A1C_04840.

    Structurei

    3D structure databases

    ProteinModelPortaliA8GPA1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi53 – 6311"HIGH" regionAdd
    BLAST
    Motifi628 – 6325"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiYETLYEC.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 2 hits.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR022439. RPE4.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    TIGR03777. RPE4. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8GPA1-1 [UniParc]FASTAAdd to Basket

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    MANTKYYPEV SSNANFAAIE REILKFWQDN NIFQKSIDGR NGESEFIFYD     50
    GPPFANGLPH YGHLLTGFIK DVYARYQTVK GKKVERRFGW DCHGLPAEMQ 100
    SEKELGISGR LAITNFGIEK FNAHCRASVM EYASDWEEYV TRQARWVDFK 150
    NSYKTMDKNF MESVLWAFKE LYNKGLLCES MRVVPYSWAC ETPLSNFETR 200
    LDNSYRERSD KAVTVSFVLR DKLHEIPAFA GMISRESEMT VGGDYQEYRI 250
    LTWTTTPWTL PSNLAIAVGS DIDYALVPQE NICYIIAASS VSKYAKELGL 300
    SGEENFEIIK GSQLQGLRYK PLFDYFEHHP NSFKIFDVDF VVEGDGTGVV 350
    HMAPGFGEDD QILCESKGIS LVCPVDNSGK FTKEIPDLEG VQVFDANDKI 400
    IIKLKEQGNW LKTEQYIHNY PHCWRTDTPL IYKAVPSWYV KVTQFKDRMV 450
    ELNQQINWIP HHVKDNLFGK WLENARDWSI SRNRFWGTPL PVWKSDDPKY 500
    PRIDVYGSIE ELEKDFGVKV TDLHRPFIDK LTRPNPNDPT GKSTMRRIED 550
    VFDCWFESGS MPYGQAHYPF ENKEWFEDHF PADFIVEYSA QTRGWFYTLM 600
    VLSTALFDRP PFLNCICHGV ILDATGQKLS KRLNNYADPL ELFDQYGSDA 650
    LRVTMLSSNI VKGQELLIDK DGKMVFDTLR LFIKPIWSSY HFFTMYANAD 700
    SLKGEISFAS ENVLDVYILS KLKIAVSKIE ESLDNFDTQT AYHAVLEFFE 750
    VLNNWYIRRS RARFWKSEKD TDKQNAYNTL YSCLKTMAIA MSALVPMISE 800
    AIYKGLCHCE ETSTLSSRDL IAGSSKSINN LNPVVKPRDY TPSVHHNDQI 850
    SVHLCNYPTL SDFEINHELV ATMDNVLDIC SNSLFIRSTK NIRVRQPLAS 900
    ITIISKHNND LKAFENLIKD EINVKSVIYC DDLENYASKK LSINFPMLGK 950
    RLPAKMKEII AASKKGDWKA IAGGLTICGE TLNNEEYKLI LEPYSHIKGA 1000
    ASFENNSSLL ILDLELTAEL IEEGYARDIV RFIQQARKDA GFSITDRILI 1050
    EIISEFDLSE IIYNYGDFIT EQTLGEFSKN FTPDYVSKVE LEDHPIQLKI 1100
    KKS 1103
    Length:1,103
    Mass (Da):126,771
    Last modified:November 13, 2007 - v1
    Checksum:i2D5D718E96221D88
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000847 Genomic DNA. Translation: ABV75226.1.
    RefSeqiYP_001493734.1. NC_009881.1.

    Genome annotation databases

    EnsemblBacteriaiABV75226; ABV75226; A1C_04840.
    GeneIDi5645801.
    KEGGirak:A1C_04840.
    PATRICi17876936. VBIRicAka50705_1003.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000847 Genomic DNA. Translation: ABV75226.1 .
    RefSeqi YP_001493734.1. NC_009881.1.

    3D structure databases

    ProteinModelPortali A8GPA1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 293614.A1C_04840.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV75226 ; ABV75226 ; A1C_04840 .
    GeneIDi 5645801.
    KEGGi rak:A1C_04840.
    PATRICi 17876936. VBIRicAka50705_1003.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi YETLYEC.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci RAKA293614:GI4A-913-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 2 hits.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR022439. RPE4.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    TIGR03777. RPE4. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Rickettsia akari."
      Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Hartford.

    Entry informationi

    Entry nameiSYI_RICAH
    AccessioniPrimary (citable) accession number: A8GPA1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3