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Protein

NAD kinase

Gene

nadK

Organism
Rickettsia akari (strain Hartford)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei44 – 441Proton acceptorUniRule annotation
Binding sitei49 – 491NADUniRule annotation
Binding sitei144 – 1441NADUniRule annotation
Binding sitei152 – 1521NAD; via carbonyl oxygenUniRule annotation
Binding sitei216 – 2161NADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi44 – 452NADUniRule annotation
Nucleotide bindingi114 – 1152NADUniRule annotation
Nucleotide bindingi155 – 1606NADUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-HAMAP
  3. NAD+ kinase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. NAD metabolic process Source: InterPro
  2. NADP biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciRAKA293614:GI4A-621-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:A1C_03295
OrganismiRickettsia akari (strain Hartford)
Taxonomic identifieri293614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000006830: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 255255NAD kinasePRO_1000005438Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi293614.A1C_03295.

Structurei

3D structure databases

ProteinModelPortaliA8GNH0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000139847.
KOiK00858.
OMAiHESTVSI.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

A8GNH0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNINKIALIY NQNSKHLAII EEIKKLYNYC KIEEAEAIIV IGGDGALLHN
60 70 80 90 100
IHRYMHLNIP FYGVNLGNLG FLMNPLDTKN LSQNIHESTV SILNPLLMQA
110 120 130 140 150
EDISGQIYTA LAINEVSIFR KTNQAAKFRI DVNGIERMSE LVADGALIAT
160 170 180 190 200
PAGSSAYNLS AGGPILPLES NMLCLTPICS FRPRRWHGAL LLASATIQFK
210 220 230 240 250
ILNTNKRPVN ATADFQEFNN ITNVTVKSTT DTPIKLLFNK NHTLEDRIIK

EQFGG
Length:255
Mass (Da):28,306
Last modified:November 13, 2007 - v1
Checksum:i5BBEAD4E08201A79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000847 Genomic DNA. Translation: ABV74945.1.
RefSeqiWP_012149578.1. NC_009881.1.
YP_001493453.1. NC_009881.1.

Genome annotation databases

EnsemblBacteriaiABV74945; ABV74945; A1C_03295.
GeneIDi5644699.
KEGGirak:A1C_03295.
PATRICi17876267. VBIRicAka50705_0681.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000847 Genomic DNA. Translation: ABV74945.1.
RefSeqiWP_012149578.1. NC_009881.1.
YP_001493453.1. NC_009881.1.

3D structure databases

ProteinModelPortaliA8GNH0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi293614.A1C_03295.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV74945; ABV74945; A1C_03295.
GeneIDi5644699.
KEGGirak:A1C_03295.
PATRICi17876267. VBIRicAka50705_0681.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000139847.
KOiK00858.
OMAiHESTVSI.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciRAKA293614:GI4A-621-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Rickettsia akari."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hartford.

Entry informationi

Entry nameiNADK_RICAH
AccessioniPrimary (citable) accession number: A8GNH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: February 4, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.