Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A8GNE3 (SYL_RICAH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:A1C_03160
OrganismRickettsia akari (strain Hartford) [Complete proteome] [HAMAP]
Taxonomic identifier293614 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length870 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 870870Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009416

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif602 – 6065"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6051ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8GNE3 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: BD373D1DAB2FC9D3

FASTA870100,472
        10         20         30         40         50         60 
MNQIEQKWQQ IWHDEKAFEV SNESSKPKYY VLAMLPYPSG KIHLGHVRNY SIGDVIARFM 

        70         80         90        100        110        120 
TMQGFNVLHP MGWDAFGLPA ENAAIKNNSH PKDWTYSNIE NMKKQLKSMG FSYAWSREIN 

       130        140        150        160        170        180 
SCDPQYYKHE QKFFLELYER NLAYQKESLV NWDPVDNTVL ANEQVVDGRG WRSGAVVEKR 

       190        200        210        220        230        240 
YLKQWFLKIT DYAEELLNEI QNLKEWPEAV RSMQEKWIGK SIGANFHFKI KDNEDTTIEV 

       250        260        270        280        290        300 
FSTKPETIFG AGFIGIAFNH PIIERLVSKT PEISNFIAKC ANITCSVELD KAEKEGVFTG 

       310        320        330        340        350        360 
LYVTHPFDSN IVLPVIITNF VLMDYGTGAV FGCPAHDERD HELAVKMNLT IKKVIETDID 

       370        380        390        400        410        420 
VQKTPYTEDG ILVNSNFLNG LTSNEAKQKV IEEFEKLEIG KCTINYRLKD WGISRQRFWG 

       430        440        450        460        470        480 
CPIPMIHCEA CGIVPVPYKD LPVTLPDDVS FDGHGNPLAH HPSWKHVNCP KCNKPAIRET 

       490        500        510        520        530        540 
DTFDTFFESS WYFTRYCNSN ATEMTDKKAC DYWLPVDKYI GGIEHAVMHL LYARFFTKVM 

       550        560        570        580        590        600 
NEQHYVSVRE PFKGLFTQGM VLHATYKDEH NNWLYPEEVV KKGNEFFRKE SNDRVVQGRI 

       610        620        630        640        650        660 
EKMSKSKKNL IDLETMQKQY GADAIRLFVL SDSPPEKDLE WFVSGIEGCS RFINKLEQMF 

       670        680        690        700        710        720 
EAIKFLYNEA NKEEFEGNTE RSTAAYTLVR EDASTGSMYK LPLEASYINK ELNRLVHFTI 

       730        740        750        760        770        780 
KHVAEDIKHF ALNRAIARMR ELANAISSEI SKDKIDVNTV RHGFNILVQL LNPFIPHITE 

       790        800        810        820        830        840 
EIWQKLGNKE RLYKSDFPAF DESMLELDTY IMAVQVNGKL RDTYKFKTAA SEDAIKQITV 

       850        860        870 
NLPKVQKFLA GKEPKKIILV PRKIVNIIVN 

« Hide

References

[1]"Complete genome sequence of Rickettsia akari."
Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hartford.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000847 Genomic DNA. Translation: ABV74918.1.
RefSeqYP_001493426.1. NC_009881.1.

3D structure databases

ProteinModelPortalA8GNE3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING293614.A1C_03160.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV74918; ABV74918; A1C_03160.
GeneID5644803.
KEGGrak:A1C_03160.
PATRIC17876213. VBIRicAka50705_0654.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycRAKA293614:GI4A-594-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR005728. Rickett_RPE.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
TIGR01045. RPE1. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RICAH
AccessionPrimary (citable) accession number: A8GNE3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries