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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Rickettsia akari (strain Hartford)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei605 – 6051ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRAKA293614:GI4A-594-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:A1C_03160
OrganismiRickettsia akari (strain Hartford)
Taxonomic identifieri293614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
ProteomesiUP000006830 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 870870Leucine--tRNA ligasePRO_1000009416Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi293614.A1C_03160.

Structurei

3D structure databases

ProteinModelPortaliA8GNE3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi36 – 4611"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR005728. Rickett_RPE.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
TIGR01045. RPE1. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GNE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQIEQKWQQ IWHDEKAFEV SNESSKPKYY VLAMLPYPSG KIHLGHVRNY
60 70 80 90 100
SIGDVIARFM TMQGFNVLHP MGWDAFGLPA ENAAIKNNSH PKDWTYSNIE
110 120 130 140 150
NMKKQLKSMG FSYAWSREIN SCDPQYYKHE QKFFLELYER NLAYQKESLV
160 170 180 190 200
NWDPVDNTVL ANEQVVDGRG WRSGAVVEKR YLKQWFLKIT DYAEELLNEI
210 220 230 240 250
QNLKEWPEAV RSMQEKWIGK SIGANFHFKI KDNEDTTIEV FSTKPETIFG
260 270 280 290 300
AGFIGIAFNH PIIERLVSKT PEISNFIAKC ANITCSVELD KAEKEGVFTG
310 320 330 340 350
LYVTHPFDSN IVLPVIITNF VLMDYGTGAV FGCPAHDERD HELAVKMNLT
360 370 380 390 400
IKKVIETDID VQKTPYTEDG ILVNSNFLNG LTSNEAKQKV IEEFEKLEIG
410 420 430 440 450
KCTINYRLKD WGISRQRFWG CPIPMIHCEA CGIVPVPYKD LPVTLPDDVS
460 470 480 490 500
FDGHGNPLAH HPSWKHVNCP KCNKPAIRET DTFDTFFESS WYFTRYCNSN
510 520 530 540 550
ATEMTDKKAC DYWLPVDKYI GGIEHAVMHL LYARFFTKVM NEQHYVSVRE
560 570 580 590 600
PFKGLFTQGM VLHATYKDEH NNWLYPEEVV KKGNEFFRKE SNDRVVQGRI
610 620 630 640 650
EKMSKSKKNL IDLETMQKQY GADAIRLFVL SDSPPEKDLE WFVSGIEGCS
660 670 680 690 700
RFINKLEQMF EAIKFLYNEA NKEEFEGNTE RSTAAYTLVR EDASTGSMYK
710 720 730 740 750
LPLEASYINK ELNRLVHFTI KHVAEDIKHF ALNRAIARMR ELANAISSEI
760 770 780 790 800
SKDKIDVNTV RHGFNILVQL LNPFIPHITE EIWQKLGNKE RLYKSDFPAF
810 820 830 840 850
DESMLELDTY IMAVQVNGKL RDTYKFKTAA SEDAIKQITV NLPKVQKFLA
860 870
GKEPKKIILV PRKIVNIIVN
Length:870
Mass (Da):100,472
Last modified:November 13, 2007 - v1
Checksum:iBD373D1DAB2FC9D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000847 Genomic DNA. Translation: ABV74918.1.
RefSeqiWP_012149551.1. NC_009881.1.

Genome annotation databases

EnsemblBacteriaiABV74918; ABV74918; A1C_03160.
KEGGirak:A1C_03160.
PATRICi17876213. VBIRicAka50705_0654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000847 Genomic DNA. Translation: ABV74918.1.
RefSeqiWP_012149551.1. NC_009881.1.

3D structure databases

ProteinModelPortaliA8GNE3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi293614.A1C_03160.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV74918; ABV74918; A1C_03160.
KEGGirak:A1C_03160.
PATRICi17876213. VBIRicAka50705_0654.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciRAKA293614:GI4A-594-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR005728. Rickett_RPE.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
TIGR01045. RPE1. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete genome sequence of Rickettsia akari."
    Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Hartford.

Entry informationi

Entry nameiSYL_RICAH
AccessioniPrimary (citable) accession number: A8GNE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 13, 2007
Last modified: July 22, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.