Reviewed, UniProtKB/Swiss-Prot A8GN11 (SYE1_RICAH)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	A1C_02435

Organism

Rickettsia akari (strain Hartford) [Complete proteome] [HAMAP]

Taxonomic identifier

293614 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367752
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022
	Motif	240 – 244	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	243	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GN11-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: F765E80513E53AC9	FASTA	447	51,873
	10 20 30 40 50 60 MTKVITRFAP SPTGMLHVGN IRVALLNWLY AKKHNGQFIL RFDDTDLERS KQEYKDAIKD 70 80 90 100 110 120 DLKFLNLHWD QTFNQLSRLS RYDEIKNLLL DKKRLYACYE TPDELELKRK FQLSKGLPPM 130 140 150 160 170 180 YDRASLNLTE EQTEKYIEQG RNPHYRFLVN HEPISWHDMI KGEIQYDGKA LSDPIVIRAD 190 200 210 220 230 240 GSMTYMLCSV IDDIDYDITH IIRGEDHVSN TAIQIQMFEA LNNTPTTFGH LSLIINKDEK 250 260 270 280 290 300 ISKRVGGFEI TTLRKEIGIE AMAIASFFSL LGSSAQILPY KSMEKLINQF EISSFSKSPT 310 320 330 340 350 360 IYQPKDLERL NHKLLISLDF DEVKDHLKEI DAEYIDENFW LSIRPNLQKL RDVKDWWEIC 370 380 390 400 410 420 HHTPNVESLN LDQEYLKQAA ELLPQGAITK DSWSIWTKEI TNITGRKGKE LFLPLRLALT 430 440 GRESGPEIAD VLPLISREEI VKRLTSA

References

[1]	"Complete genome sequence of Rickettsia akari." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000847 Genomic DNA. Translation: ABV74786.1.
RefSeq	YP_001493294.1.
3D structure databases
SMR	A8GN11. Positions 3-447.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GN11.
Genome annotation databases
GeneID	5645148.
GenomeReviews	Gene locus A1C_02435 in contig CP000847_GR.
KEGG	rak:A1C_02435.
NMPDR	fig|293614.3.peg.452.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	LRLDDTD.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_RICAH

Accession

Primary (citable) accession number: A8GN11

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 13, 2007
Last modified:	February 9, 2010
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)