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A8GMP8 (DDL_RICAH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:A1C_01810
OrganismRickettsia akari (strain Hartford) [Complete proteome] [HAMAP]
Taxonomic identifier293614 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 320320D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341169

Regions

Domain120 – 314195ATP-grasp
Nucleotide binding147 – 19852ATP By similarity

Sites

Metal binding2671Magnesium or manganese 1 By similarity
Metal binding2811Magnesium or manganese 1 By similarity
Metal binding2811Magnesium or manganese 2 By similarity
Metal binding2831Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8GMP8 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 11CE6A1FD4788497

FASTA32035,666
        10         20         30         40         50         60 
MQYQTHWIEH SVVKILSTTG KNHIALVAGG MSAEREVSLV SSEGVQQALI ALGYKVTFID 

        70         80         90        100        110        120 
MGADIAVKLQ EIKPDIVFNC LHGTYGEDGC LPGLLNIMRI PYTHSGVLSS ALAFNKIHSS 

       130        140        150        160        170        180 
SWFFANSINT AESIVVSKSY NINTDPMKRP YVIKPLTQGS SIGVEVIFEE DDFNFADYDF 

       190        200        210        220        230        240 
PYGDQVVIER YIKGREFQVA VLNSKALGAL EIKLIKNRFY DYETKYTEGF AEHLCPAPLH 

       250        260        270        280        290        300 
ANLYEKLLIE SEKIYKTMNC KGPARAEFIL EEQTNKLYAL EINTHPGMTP LSIVPEIAAY 

       310        320 
AGINFTNLIA EIIKTASFES 

« Hide

References

[1]"Complete genome sequence of Rickettsia akari."
Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Hartford.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000847 Genomic DNA. Translation: ABV74673.1.
RefSeqYP_001493181.1. NC_009881.1.

3D structure databases

ProteinModelPortalA8GMP8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING293614.A1C_01810.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV74673; ABV74673; A1C_01810.
GeneID5645119.
KEGGrak:A1C_01810.
PATRIC17875635. VBIRicAka50705_0370.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAKYTEGFA.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycRAKA293614:GI4A-345-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_RICAH
AccessionPrimary (citable) accession number: A8GMP8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: November 13, 2007
Last modified: February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways