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Protein

UvrABC system protein B

Gene

uvrB

Organism
Rickettsia akari (strain Hartford)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 45ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease, Helicase, Hydrolase
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:A1C_01490
OrganismiRickettsia akari (strain Hartford)
Taxonomic identifieri293614 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesRickettsiaceaeRickettsieaeRickettsiaspotted fever group
Proteomesi
  • UP000006830 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000779131 – 661UvrABC system protein BAdd BLAST661

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi293614.A1C_01490.

Structurei

3D structure databases

ProteinModelPortaliA8GMJ1.
SMRiA8GMJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 182Helicase ATP-bindingUniRule annotationAdd BLAST158
Domaini430 – 592Helicase C-terminalUniRule annotationAdd BLAST163
Domaini621 – 656UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi91 – 114Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
HOGENOMiHOG000073580.
KOiK03702.
OMAiRYMHSEI.
OrthoDBiPOG091H02LK.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

A8GMJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNHFSLISEY KPAGDQPKAI DEIIAGLNSK KRSQMLLGIT GSGKTFTMAN
60 70 80 90 100
IIERTNRPTL IMAHNKTLAA QIYSEMKSIF PKNAVEYFVS YYDYYQPEAY
110 120 130 140 150
IARTDTFIEK DSSINEHIDL MRHSATRSLL ERRDVIVVAS VSCIYGLGSP
160 170 180 190 200
DLYYQMTVNL EPGKSYPRDK LLNDLINLQY KRNDMGFERG CFRVKGDNID
210 220 230 240 250
IFPSHYSDKA WRLSFFSNEL EYIHEFDPLT GEKLAKLDKA IVFGHSHFVM
260 270 280 290 300
PQDTVNNAIS GIEEELQKRL EFLKSQNKLL ETQRLNQRTQ YDLEMLTETG
310 320 330 340 350
SCKGVENYSR FFTGRNAGEP PPTLFEYLPK DALLFVDESH VSVPQIKAMY
360 370 380 390 400
NSDRARKEVL VEHGFRLPSA LDNRPLKFEE WEKFRPQTVF VSATPGPFEL
410 420 430 440 450
EETGGTVVEL IIRPTGLLDP ECIIKPATKQ VEDLISEIQA TVAKGLRVLV
460 470 480 490 500
TTLTKKMAED LTAYLQELKY KTSYLHSNVH TLERIEILQD LRQGTIDILV
510 520 530 540 550
GINLLREGLD IPECGLVAIL DADKEGFLRS EVSLIQTIGR AARNSEGRVI
560 570 580 590 600
LYADKMTKSI DKALSETLRR RQIQQEYNEK HGIIPKTINR AIHALATLER
610 620 630 640 650
VDSKCDTKQA HTLFDNTAKL KANINKLNKE MLKAASNLEF EQAAKLRDQL
660
KTLEAAAREL S
Length:661
Mass (Da):75,233
Last modified:November 13, 2007 - v1
Checksum:i469761CEF5D13D0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000847 Genomic DNA. Translation: ABV74616.1.
RefSeqiWP_012149250.1. NC_009881.1.

Genome annotation databases

EnsemblBacteriaiABV74616; ABV74616; A1C_01490.
KEGGirak:A1C_01490.

Similar proteinsi

Entry informationi

Entry nameiUVRB_RICAH
AccessioniPrimary (citable) accession number: A8GMJ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: October 25, 2017
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families