A8GM78 (LEP_RICAH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	A1C_00865

Organism

Rickettsia akari (strain Hartford) [Complete proteome] [HAMAP]

Taxonomic identifier

293614 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	Signal peptidase I		PRO_0000316271
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Potential
	Topological domain	42 – 266	225	Periplasmic Potential
Sites
	Active site	45	1	By similarity
	Active site	108	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GM78 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 8EA822E12B01BF70	FASTA	266	31,328
	10 20 30 40 50 60 MQTDNTKSNT NKTAKQEWWS CAFVICIALL IRILIMEPFT VPTGSMKATI LENDYIFSTK 70 80 90 100 110 120 YSYGYSNYSL SFFDFIPLFK GRIFAREPER GDIVVFRPPN DMNVRYIKRL IGLPGDKIQL 130 140 150 160 170 180 IDDVIYINDK KIERTEVGTY TSEDGIKYLK FKETLPNGRT YFSYKLAPIF SVIYNDRYGN 190 200 210 220 230 240 TDVFYVPEGK YFFLGDNRDQ SNDSRVNLGF VPFENFIAKA QFIWFSTKIN WWDNDIGVMN 250 260 LVLRLKPWIE SVRLNRIFRN LYNTDE

References

[1]	"Complete genome sequence of Rickettsia akari." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hartford.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000847 Genomic DNA. Translation: ABV74503.1.
RefSeq	YP_001493011.1. NC_009881.1.
3D structure databases
ProteinModelPortal	A8GM78.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GM78.
Protein family/group databases
MEROPS	S26.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5645095.
GenomeReviews	Gene locus A1C_00865 in contig CP000847_GR.
KEGG	rak:A1C_00865.
NMPDR	fig|293614.3.peg.161.
PATRIC	17875239. VBIRicAka50705_0177.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0681.
HOGENOM	HBG596607.
OMA	KPWIESV.
ProtClustDB	CLSK870751.
Enzyme and pathway databases
BioCyc	RAKA293614:A1C_00865-MONOMER.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 2 hits.
KO	K03100.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. Sigpep_I_bact. 1 hit.
PROSITE	PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_RICAH

Accession

Primary (citable) accession number: A8GM78

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program