A8GM19 (QUEF_RICAH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	A1C_00555

Organism

Rickettsia akari (strain Hartford) [Complete proteome] [HAMAP]

Taxonomic identifier

293614 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › spotted fever group

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm Probable HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000062356

Sequences

	Sequence		Length	Mass (Da)
	A8GM19 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: F248107A23EFF3D8	FASTA	273	31,112
	10 20 30 40 50 60 MPLSTSLLGK TSTYKDSYDA TLLFKIPRIN NRNALGINSN NLPFYGVDIW NTYELSCINK 70 80 90 100 110 120 KGKPWVGVGT FYIPTDSENI VESKSFKLYL NSFNNFVVES VEELERIILQ DLSNVTHAKV 130 140 150 160 170 180 TGRIFPINTK IAFGVPSGKN IDALDIVCNN YGPPDNSLVE YEEVLVEEEI NSNLLKSNCL 190 200 210 220 230 240 VTGQPDWGSI VIKYKGKKLK HDSFLKYLIS FRNYHEFAEQ CAERIFTDIK NAIKPDFLSL 250 260 270 YIVYTRRGGI DICPYRSTDK SYSLPSDKRL IRQ

References

[1]	"Complete genome sequence of Rickettsia akari." Madan A., Fahey J., Helton E., Ketteman M., Madan A., Rodrigues S., Sanchez A., Whiting M., Dasch G., Eremeeva M. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hartford.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000847 Genomic DNA. Translation: ABV74444.1.
RefSeq	YP_001492952.1. NC_009881.1.
3D structure databases
ProteinModelPortal	A8GM19.
ModBase	Search...
Protein-protein interaction databases
STRING	A8GM19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5645671.
GenomeReviews	Gene locus A1C_00555 in contig CP000847_GR.
KEGG	rak:A1C_00555.
NMPDR	fig|293614.3.peg.99.
PATRIC	17875111. VBIRicAka50705_0115.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG289139.
OMA	CVERIFC.
ProtClustDB	PRK11792.
Enzyme and pathway databases
BioCyc	RAKA293614:A1C_00555-MONOMER.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_RICAH

Accession

Primary (citable) accession number: A8GM19

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program