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Protein

Argininosuccinate synthase

Gene

argG

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argI), Ornithine carbamoyltransferase (argI)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei92CitrullineUniRule annotation1
Binding sitei97CitrullineUniRule annotation1
Binding sitei122ATP; via amide nitrogenUniRule annotation1
Binding sitei124AspartateUniRule annotation1
Binding sitei128AspartateUniRule annotation1
Binding sitei128CitrullineUniRule annotation1
Binding sitei129AspartateUniRule annotation1
Binding sitei132CitrullineUniRule annotation1
Binding sitei181CitrullineUniRule annotation1
Binding sitei190CitrullineUniRule annotation1
Binding sitei266CitrullineUniRule annotation1
Binding sitei278CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 20ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Spro_4779
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeSerratia
Proteomesi
  • UP000007074 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000570441 – 406Argininosuccinate synthaseAdd BLAST406

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi399741.Spro_4779

Structurei

3D structure databases

ProteinModelPortaliA8GL82
SMRiA8GL82
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH Bacteria
COG0137 LUCA
HOGENOMiHOG000230093
KOiK01940
OMAiGVGRIDM
OrthoDBiPOG091H01U0

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.1260.10, 2 hits
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

A8GL82-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNQGIKKIV LAYSGGLDTS AIIPWLKENY GGCEVVAFVA DIGQERSDLE
60 70 80 90 100
GVEQKALQSG ASECHVVDLR EEFIRDYVYP VLQTGALYEG SYLLGTSMAR
110 120 130 140 150
PIIAKAQVEL ALKVGADAVC HGATGKGNDQ VRFETTYTAL APHLKVVAPW
160 170 180 190 200
REWNLRSREA LLDYLKERNI PTTASLEKIY SRDENAWHIS TEGGVLESPW
210 220 230 240 250
NAPNKDCWVW TVDPQEAPDQ PEQVTITVEK GCVVAVNGEA LSPYKCLETL
260 270 280 290 300
NVLGAKHGVG RIDIVENRLV GIKSRGCYET PGGTIMVAAL RGVEQLVLDR
310 320 330 340 350
DSFKWREQLG LEMSYVVYDG RWFAPLRRSL QASAEALAEE VNGEVVLQLY
360 370 380 390 400
KGQVTAIQKK SANSLYSEEF ATFGEDEVYD HSHAGGFIRL FSLSSRIRAL

NEIKNK
Length:406
Mass (Da):44,973
Last modified:November 13, 2007 - v1
Checksum:iC1DBEC2A22CB2E39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA Translation: ABV43872.1
RefSeqiWP_012147453.1, NC_009832.1

Genome annotation databases

EnsemblBacteriaiABV43872; ABV43872; Spro_4779
KEGGispe:Spro_4779

Similar proteinsi

Entry informationi

Entry nameiASSY_SERP5
AccessioniPrimary (citable) accession number: A8GL82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: March 28, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health