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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei405NucleophileUniRule annotation1
Active sitei458Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:Spro_4647
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeSerratia
Proteomesi
  • UP000007074 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000619931 – 7281,4-alpha-glucan branching enzyme GlgBAdd BLAST728

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi399741.Spro_4647

Structurei

3D structure databases

ProteinModelPortaliA8GKV0
SMRiA8GKV0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9C Bacteria
COG0296 LUCA
HOGENOMiHOG000283037
KOiK00700
OMAiDKRRQPI
OrthoDBiPOG091H08D1

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
HAMAPiMF_00685 GlgB, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006407 GlgB
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 2 hits
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 2 hits
TIGRFAMsiTIGR01515 branching_enzym, 1 hit

Sequencei

Sequence statusi: Complete.

A8GKV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPVLPDRDVI DQLFSGNSAD PFSLLGMHAA DNGLQVRALL PDASEVWLLE
60 70 80 90 100
QQTGRRLVQL NCDDARGFFS ATVPRRKTPF RYQFEVRWQD HQQIVDDPYR
110 120 130 140 150
FGTLLQDIDS WLLAEGTHLR PYERLGAHLS TLDDVEGVSF AVWAPNAQRV
160 170 180 190 200
SVVGEFNFWD GRRHPMRLRR ENGIWELFLP GVKAGQLYKY EIIDCYGNTQ
210 220 230 240 250
LKADPYAFEA QMRPDTASLV TPLPEVVENT PQRQRANDFD RPISIYEVHL
260 270 280 290 300
GSWRRHTDDN FWLSYGELAV QLIDYVKDMG FTHIELLPIN EHPFDGSWGY
310 320 330 340 350
QPLGLYAPTR RFGTPADFKD FVAAAHRAGI NVILDWVPGH FPSDAYGLAN
360 370 380 390 400
FDGTALYEYA DPREGFHQDW NTLIYNYGRH EVRNYLAGNA LYWLERYGID
410 420 430 440 450
ALRVDAVASM IYRDYSRAEG EWVPNYYGGN ENLEAIAFLR YTNQSVGKER
460 470 480 490 500
PGAVTLAEES TDYPGVTLPP ESNGLGFHYK WNLGWMHDTL NYMQCDPVHR
510 520 530 540 550
KYHHNQMTFG MLYAYTENFV LPISHDEVVH GKKSILDRMP GDAWQKFANL
560 570 580 590 600
RAYYGFMWAH PGKKLLFMGC EFAQGREWNF DSSLDWHLLE GLDGWHHGVQ
610 620 630 640 650
RLVRDLNHCY QQQAPLYERD YRPDGFEWLV VDDHDNSVFA FARYDAHGNE
660 670 680 690 700
LIAVSNFTPV PRHHYRIGIS RPGEYREILN TDSHHYHGSN TGNQGRVVSE
710 720
QIGNHGREHS ISVTVPPLAT IYLLREAQ
Length:728
Mass (Da):83,974
Last modified:November 13, 2007 - v1
Checksum:iE6D985C8D29312AA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA Translation: ABV43740.1

Genome annotation databases

EnsemblBacteriaiABV43740; ABV43740; Spro_4647
KEGGispe:Spro_4647

Similar proteinsi

Entry informationi

Entry nameiGLGB_SERP5
AccessioniPrimary (citable) accession number: A8GKV0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: March 28, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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