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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69S-adenosyl-L-methionineUniRule annotation1
Binding sitei94S-adenosyl-L-methionineUniRule annotation1
Binding sitei121S-adenosyl-L-methionineUniRule annotation1
Active sitei144By similarity1
Binding sitei144S-adenosyl-L-methionineUniRule annotation1
Binding sitei148SubstrateUniRule annotation1
Binding sitei180SubstrateUniRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Spro_4042
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeSerratia
Proteomesi
  • UP000007074 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000644071 – 239tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST239

Proteomic databases

PRIDEiA8GJ47

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi399741.Spro_4042

Structurei

3D structure databases

ProteinModelPortaliA8GJ47
SMRiA8GJ47
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 155Interaction with RNAUniRule annotation6
Regioni217 – 220Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000073968
KOiK03439
OMAiPDPWHKS
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

A8GJ47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINDVISPEF DENGRAMRRI RSFVRRQGRL TKGQQHALDN YWPVMGVEYQ
60 70 80 90 100
AEPVDITALF GRDAPTVLEI GFGMGASLVT MAGHNPQQNF LGIEVHSPGV
110 120 130 140 150
GACLADATEA ELSNLRVMCH DAVEVLENMI PDGSLDMVQL FFPDPWHKAR
160 170 180 190 200
HNKRRIVQTP FVELVRRKLK VGGVFHMATD WQPYAEHMLE VMNGISGYRN
210 220 230
LSSDNDYVPR PDSRPLTKFE LRGQRLGHGV WDLMFERKE
Length:239
Mass (Da):27,172
Last modified:November 13, 2007 - v1
Checksum:i679374ADE54B088C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA Translation: ABV43137.1
RefSeqiWP_012146744.1, NC_009832.1

Genome annotation databases

EnsemblBacteriaiABV43137; ABV43137; Spro_4042
KEGGispe:Spro_4042

Similar proteinsi

Entry informationi

Entry nameiTRMB_SERP5
AccessioniPrimary (citable) accession number: A8GJ47
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 23, 2018
This is version 62 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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