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A8GIE8

- GLND_SERP5

UniProt

A8GIE8 - GLND_SERP5

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciSPRO399741:GI55-3872-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:Spro_3792
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
ProteomesiUP000007074: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000059226Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi399741.Spro_3792.

Structurei

3D structure databases

ProteinModelPortaliA8GIE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini470 – 603134HDUniRule annotationAdd
BLAST
Domaini711 – 79282ACT 1UniRule annotationAdd
BLAST
Domaini818 – 89275ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 351351UridylyltransferaseAdd
BLAST
Regioni352 – 710359Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GIE8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSENFRQQDT SVISPQAVPV QPEYPNAYGD EEINCIALKQ RLEQFQLWLA
60 70 80 90 100
AAFNAGSSAE SLVAARSDFI DRLLRRLWVF YGFEDIPETA LVAVGGYGRG
110 120 130 140 150
ELHPLSDIDV LVLSQRRLNE QQSQRVGEFI TLLWDLKLEV GHSVRTLEEC
160 170 180 190 200
LLEGLADLTV ATNLIESRMI CGDVALFLQM QKHIFSDGFW PSPQFFHAKV
210 220 230 240 250
VEQQERHQRY HGTSYNLEPD IKSSPGGLRD IHTLLWVARR HFGATSLDEM
260 270 280 290 300
VGFGFLTQAE RNELNECQSF LWRIRFALHL VLPRYDNRLL FDRQLNVAQL
310 320 330 340 350
LRYEGEGNEP VERMMKDFYR MTRRVGELNH MLLQLFDEAI LALDATEKPR
360 370 380 390 400
PLNDDFQLRG DLIDLRDETL FIRQPESIMR MFYLMVRNRE IKGIYSTTVR
410 420 430 440 450
QLRHARRHLK QPLCTIPEAR ELFMAILRHP GAVSRALVPM HRHSVLWAYM
460 470 480 490 500
PQWGKIVGQM QFDLFHAYTV DEHTIRVLQK LESFADAETR PRHPLCVELY
510 520 530 540 550
PRLPNPELLL LAALFHDIAK GRGGDHSILG AQDVLEFAEV HGLNSREAQL
560 570 580 590 600
VAWLVRCHLL MSVTAQRRDI QDPTVIQQFS AEVQSETRLR YLVCLTVADI
610 620 630 640 650
CATNETLWNS WKQSLLRELY FATEKQLRRG MQNSPDLRER VRHHRLQALA
660 670 680 690 700
LLRMDNIDEE ALHRIWSRCR ADYFLRHSPN QLAWHARHLL AHDSTKPLVL
710 720 730 740 750
VSRQATRGGT EIFIWSPDRP YLFAAVAGEM DRRNLSVHDA QIFTNRDGMA
760 770 780 790 800
MDTFIVLEPD GSPLAQDRHT AIRHALLQAI TQREYQPPRV RRPSSKLRHF
810 820 830 840 850
SVPTEVSFLP THTDRRSYLE LTALDQPGLL ARVGEVFADL NLSLHGARIS
860 870 880 890
TIGERVEDLF ILADSDRRAL NPETRRKLEQ RLTEALNPND KM
Length:892
Mass (Da):103,200
Last modified:November 13, 2007 - v1
Checksum:i8D6DC4E817EDB145
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV42888.1.
RefSeqiWP_012146496.1. NC_009832.1.
YP_001480016.1. NC_009832.1.

Genome annotation databases

EnsemblBacteriaiABV42888; ABV42888; Spro_3792.
GeneIDi5607191.
KEGGispe:Spro_3792.
PATRICi32420400. VBISerPro44537_3852.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV42888.1 .
RefSeqi WP_012146496.1. NC_009832.1.
YP_001480016.1. NC_009832.1.

3D structure databases

ProteinModelPortali A8GIE8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399741.Spro_3792.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV42888 ; ABV42888 ; Spro_3792 .
GeneIDi 5607191.
KEGGi spe:Spro_3792.
PATRICi 32420400. VBISerPro44537_3852.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci SPRO399741:GI55-3872-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 568.

Entry informationi

Entry nameiGLND_SERP5
AccessioniPrimary (citable) accession number: A8GIE8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3