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A8GIE8

- GLND_SERP5

UniProt

A8GIE8 - GLND_SERP5

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciSPRO399741:GI55-3872-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:Spro_3792
    OrganismiSerratia proteamaculans (strain 568)
    Taxonomic identifieri399741 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
    ProteomesiUP000007074: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 892892Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000059226Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi399741.Spro_3792.

    Structurei

    3D structure databases

    ProteinModelPortaliA8GIE8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini470 – 603134HDUniRule annotationAdd
    BLAST
    Domaini711 – 79282ACT 1UniRule annotationAdd
    BLAST
    Domaini818 – 89275ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 351351UridylyltransferaseAdd
    BLAST
    Regioni352 – 710359Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8GIE8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSENFRQQDT SVISPQAVPV QPEYPNAYGD EEINCIALKQ RLEQFQLWLA    50
    AAFNAGSSAE SLVAARSDFI DRLLRRLWVF YGFEDIPETA LVAVGGYGRG 100
    ELHPLSDIDV LVLSQRRLNE QQSQRVGEFI TLLWDLKLEV GHSVRTLEEC 150
    LLEGLADLTV ATNLIESRMI CGDVALFLQM QKHIFSDGFW PSPQFFHAKV 200
    VEQQERHQRY HGTSYNLEPD IKSSPGGLRD IHTLLWVARR HFGATSLDEM 250
    VGFGFLTQAE RNELNECQSF LWRIRFALHL VLPRYDNRLL FDRQLNVAQL 300
    LRYEGEGNEP VERMMKDFYR MTRRVGELNH MLLQLFDEAI LALDATEKPR 350
    PLNDDFQLRG DLIDLRDETL FIRQPESIMR MFYLMVRNRE IKGIYSTTVR 400
    QLRHARRHLK QPLCTIPEAR ELFMAILRHP GAVSRALVPM HRHSVLWAYM 450
    PQWGKIVGQM QFDLFHAYTV DEHTIRVLQK LESFADAETR PRHPLCVELY 500
    PRLPNPELLL LAALFHDIAK GRGGDHSILG AQDVLEFAEV HGLNSREAQL 550
    VAWLVRCHLL MSVTAQRRDI QDPTVIQQFS AEVQSETRLR YLVCLTVADI 600
    CATNETLWNS WKQSLLRELY FATEKQLRRG MQNSPDLRER VRHHRLQALA 650
    LLRMDNIDEE ALHRIWSRCR ADYFLRHSPN QLAWHARHLL AHDSTKPLVL 700
    VSRQATRGGT EIFIWSPDRP YLFAAVAGEM DRRNLSVHDA QIFTNRDGMA 750
    MDTFIVLEPD GSPLAQDRHT AIRHALLQAI TQREYQPPRV RRPSSKLRHF 800
    SVPTEVSFLP THTDRRSYLE LTALDQPGLL ARVGEVFADL NLSLHGARIS 850
    TIGERVEDLF ILADSDRRAL NPETRRKLEQ RLTEALNPND KM 892
    Length:892
    Mass (Da):103,200
    Last modified:November 13, 2007 - v1
    Checksum:i8D6DC4E817EDB145
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000826 Genomic DNA. Translation: ABV42888.1.
    RefSeqiYP_001480016.1. NC_009832.1.

    Genome annotation databases

    EnsemblBacteriaiABV42888; ABV42888; Spro_3792.
    GeneIDi5607191.
    KEGGispe:Spro_3792.
    PATRICi32420400. VBISerPro44537_3852.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000826 Genomic DNA. Translation: ABV42888.1 .
    RefSeqi YP_001480016.1. NC_009832.1.

    3D structure databases

    ProteinModelPortali A8GIE8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399741.Spro_3792.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV42888 ; ABV42888 ; Spro_3792 .
    GeneIDi 5607191.
    KEGGi spe:Spro_3792.
    PATRICi 32420400. VBISerPro44537_3852.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci SPRO399741:GI55-3872-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 2 hits.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 568.

    Entry informationi

    Entry nameiGLND_SERP5
    AccessioniPrimary (citable) accession number: A8GIE8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3