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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (Spro_2352), Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase (lpxD)
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:Spro_3777
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeSerratia
Proteomesi
  • UP000007074 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000607741 – 382Lipid-A-disaccharide synthaseAdd BLAST382

Interactioni

Protein-protein interaction databases

STRINGi399741.Spro_3777

Structurei

3D structure databases

ProteinModelPortaliA8GID3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018003
KOiK00748
OMAiPTVWAWR
OrthoDBiPOG091H01NW

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

A8GID3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNRPLTIGL VAGETSGDIL GAGLIRALKA QIPDARFVGV AGPLMQAEGC
60 70 80 90 100
ETWYEMEELA VMGVVEVLER LPRLLKIRKD LTRRFSDLAP DVFVGIDAPD
110 120 130 140 150
FNITLEGRLK QRGIRTIHYV SPSVWAWRQK RVFKIGKATD LVLAFLPFEK
160 170 180 190 200
AFYDRFNVPC RFIGHTMADA MPLQPDKLAA RAKLGIAADA RCLALLPGSR
210 220 230 240 250
GAEVEMLSAD FLKTAQLLRT RYPELELVVP LVNAKRREQF ERIKAEVAPE
260 270 280 290 300
LRVHLLNGQG REAMIASDAA LLASGTAALE CMLAKCPMVV GYRMKPFTFW
310 320 330 340 350
IAQRLVKTPY VSLPNLLAGR EIVTELLQHD CVPDKLAASV MPLLEDSPQT
360 370 380
DELKQTFLTL HQSIRCGADE QAAQAVLELA KA
Length:382
Mass (Da):42,312
Last modified:November 13, 2007 - v1
Checksum:i544FC0A596EBAC34
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA Translation: ABV42873.1
RefSeqiWP_012146482.1, NC_009832.1

Genome annotation databases

EnsemblBacteriaiABV42873; ABV42873; Spro_3777
KEGGispe:Spro_3777

Similar proteinsi

Entry informationi

Entry nameiLPXB_SERP5
AccessioniPrimary (citable) accession number: A8GID3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: March 28, 2018
This is version 57 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health