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A8GHJ4

- NAPA_SERP5

UniProt

A8GHJ4 - NAPA_SERP5

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi49 – 491Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi53 – 531Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi81 – 811Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSPRO399741:GI55-3561-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:Spro_3486
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
ProteomesiUP000007074: Chromosome

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Tat-type signalUniRule annotationAdd
BLAST
Chaini34 – 828795Periplasmic nitrate reductasePRO_5000279603Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi399741.Spro_3486.

Structurei

3D structure databases

ProteinModelPortaliA8GHJ4.
SMRiA8GHJ4. Positions 37-827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A8GHJ4-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MKLSRRDFMK ANAAVAAAAA AGLTIPTVVQ AAAGSADSIK WDKAPCRFCG
60 70 80 90 100
TGCGVLVGTQ NGRIVASQGD PEAAVNRGLS CIKGYFLPKI MYGKDRLNQP
110 120 130 140 150
LLRMKDGQYH KEGEFTPVSW QQAFDIMAEK FKQALKEKGP EAVGMFGSGQ
160 170 180 190 200
WTVWEGYAAA KLLKAGLRSN NLDPNARHCM ASAVVGFMRT FGMDEPMGCY
210 220 230 240 250
DDIEQADAFV LWGSNMAEMH PILWSRITDR RLSNEKVNVS VLSTFEHRSF
260 270 280 290 300
ELADNGMVFT PQTDLAIMNY IANYIIQNNA VDQDFLNRHV NFRRGATDIG
310 320 330 340 350
YGLRPTDPLE KAAKNPGSDA SDPMSFEEYK AFVADYTLEK TAKMSGVPED
360 370 380 390 400
QLEALAKLYA DPNIKVVSYW TMGFNQHTRG VWANNLCYNL HLLTGKISKP
410 420 430 440 450
GCGPFSLTGQ PSACGTAREV GTFAHRLPAD MVVTNEKHRQ TAEQKWQLPA
460 470 480 490 500
GTIPAKVGLH AVAQDRALKD GTLNAYWVMC NNNMQAGPNI NEDRMPGWRD
510 520 530 540 550
ARNFVVVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER RTQFWRQQVK
560 570 580 590 600
APGEAKSDLW QLVEFSKRFT VEEVWPAELL AQKPEYRGKT LYDVLFANGE
610 620 630 640 650
VNKYPLTEIP ADQLNDEARD FGFYLQKGLF EEYAGFGRGH GHDLAPFDSY
660 670 680 690 700
HQARGLRWPV VEGKETLWRY REGTDPYVKA GEEVRFYGKP DGKAVIFALP
710 720 730 740 750
FEPAAESPDK EYDLWLSTGR VLEHWHTGSM TRRVPELHRA FPQAVVFIHP
760 770 780 790 800
LDAKSRNLRR GEKVRVLSRR GELISTVETR GRNRPPRGLV YMPFFDAAQL
810 820
VNNLTLDATD PLSKEVDFKK CAVKLEKV
Length:828
Mass (Da):92,599
Last modified:November 13, 2007 - v1
Checksum:i46B8C60CD80539E9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000826 Genomic DNA. Translation: ABV42584.1.
RefSeqiYP_001479712.1. NC_009832.1.

Genome annotation databases

EnsemblBacteriaiABV42584; ABV42584; Spro_3486.
GeneIDi5604924.
KEGGispe:Spro_3486.
PATRICi32419776. VBISerPro44537_3545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000826 Genomic DNA. Translation: ABV42584.1 .
RefSeqi YP_001479712.1. NC_009832.1.

3D structure databases

ProteinModelPortali A8GHJ4.
SMRi A8GHJ4. Positions 37-827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399741.Spro_3486.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV42584 ; ABV42584 ; Spro_3486 .
GeneIDi 5604924.
KEGGi spe:Spro_3486.
PATRICi 32419776. VBISerPro44537_3545.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci SPRO399741:GI55-3561-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 568.

Entry informationi

Entry nameiNAPA_SERP5
AccessioniPrimary (citable) accession number: A8GHJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: October 29, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3