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A8GHJ4 (NAPA_SERP5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:Spro_3486
OrganismSerratia proteamaculans (strain 568) [Complete proteome] [HAMAP]
Taxonomic identifier399741 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP-Rule MF_01630

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333Tat-type signal Potential
Chain34 – 828795Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_5000279603

Regions

Domain39 – 95574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding491Iron-sulfur (4Fe-4S) By similarity
Metal binding531Iron-sulfur (4Fe-4S) By similarity
Metal binding811Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A8GHJ4 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 46B8C60CD80539E9

FASTA82892,599
        10         20         30         40         50         60 
MKLSRRDFMK ANAAVAAAAA AGLTIPTVVQ AAAGSADSIK WDKAPCRFCG TGCGVLVGTQ 

        70         80         90        100        110        120 
NGRIVASQGD PEAAVNRGLS CIKGYFLPKI MYGKDRLNQP LLRMKDGQYH KEGEFTPVSW 

       130        140        150        160        170        180 
QQAFDIMAEK FKQALKEKGP EAVGMFGSGQ WTVWEGYAAA KLLKAGLRSN NLDPNARHCM 

       190        200        210        220        230        240 
ASAVVGFMRT FGMDEPMGCY DDIEQADAFV LWGSNMAEMH PILWSRITDR RLSNEKVNVS 

       250        260        270        280        290        300 
VLSTFEHRSF ELADNGMVFT PQTDLAIMNY IANYIIQNNA VDQDFLNRHV NFRRGATDIG 

       310        320        330        340        350        360 
YGLRPTDPLE KAAKNPGSDA SDPMSFEEYK AFVADYTLEK TAKMSGVPED QLEALAKLYA 

       370        380        390        400        410        420 
DPNIKVVSYW TMGFNQHTRG VWANNLCYNL HLLTGKISKP GCGPFSLTGQ PSACGTAREV 

       430        440        450        460        470        480 
GTFAHRLPAD MVVTNEKHRQ TAEQKWQLPA GTIPAKVGLH AVAQDRALKD GTLNAYWVMC 

       490        500        510        520        530        540 
NNNMQAGPNI NEDRMPGWRD ARNFVVVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER 

       550        560        570        580        590        600 
RTQFWRQQVK APGEAKSDLW QLVEFSKRFT VEEVWPAELL AQKPEYRGKT LYDVLFANGE 

       610        620        630        640        650        660 
VNKYPLTEIP ADQLNDEARD FGFYLQKGLF EEYAGFGRGH GHDLAPFDSY HQARGLRWPV 

       670        680        690        700        710        720 
VEGKETLWRY REGTDPYVKA GEEVRFYGKP DGKAVIFALP FEPAAESPDK EYDLWLSTGR 

       730        740        750        760        770        780 
VLEHWHTGSM TRRVPELHRA FPQAVVFIHP LDAKSRNLRR GEKVRVLSRR GELISTVETR 

       790        800        810        820 
GRNRPPRGLV YMPFFDAAQL VNNLTLDATD PLSKEVDFKK CAVKLEKV 

« Hide

References

[1]"Complete sequence of chromosome of Serratia proteamaculans 568."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. expand/collapse author list , Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 568.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000826 Genomic DNA. Translation: ABV42584.1.
RefSeqYP_001479712.1. NC_009832.1.

3D structure databases

ProteinModelPortalA8GHJ4.
SMRA8GHJ4. Positions 37-827.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399741.Spro_3486.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV42584; ABV42584; Spro_3486.
GeneID5604924.
KEGGspe:Spro_3486.
PATRIC32419776. VBISerPro44537_3545.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMAHPKTRVA.
OrthoDBEOG6CVV7G.

Enzyme and pathway databases

BioCycSPRO399741:GI55-3561-MONOMER.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_SERP5
AccessionPrimary (citable) accession number: A8GHJ4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families