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A8GHJ4

- NAPA_SERP5

UniProt

A8GHJ4 - NAPA_SERP5

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Protein
Periplasmic nitrate reductase
Gene
napA, Spro_3486
Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi46 – 461Iron-sulfur (4Fe-4S) By similarity
Metal bindingi49 – 491Iron-sulfur (4Fe-4S) By similarity
Metal bindingi53 – 531Iron-sulfur (4Fe-4S) By similarity
Metal bindingi81 – 811Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciSPRO399741:GI55-3561-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:Spro_3486
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
ProteomesiUP000007074: Chromosome

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3333Tat-type signal Reviewed prediction
Add
BLAST
Chaini34 – 828795Periplasmic nitrate reductaseUniRule annotation
PRO_5000279603Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi399741.Spro_3486.

Structurei

3D structure databases

ProteinModelPortaliA8GHJ4.
SMRiA8GHJ4. Positions 37-827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 95574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiHPKTRVA.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A8GHJ4-1 [UniParc]FASTAAdd to Basket

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MKLSRRDFMK ANAAVAAAAA AGLTIPTVVQ AAAGSADSIK WDKAPCRFCG    50
TGCGVLVGTQ NGRIVASQGD PEAAVNRGLS CIKGYFLPKI MYGKDRLNQP 100
LLRMKDGQYH KEGEFTPVSW QQAFDIMAEK FKQALKEKGP EAVGMFGSGQ 150
WTVWEGYAAA KLLKAGLRSN NLDPNARHCM ASAVVGFMRT FGMDEPMGCY 200
DDIEQADAFV LWGSNMAEMH PILWSRITDR RLSNEKVNVS VLSTFEHRSF 250
ELADNGMVFT PQTDLAIMNY IANYIIQNNA VDQDFLNRHV NFRRGATDIG 300
YGLRPTDPLE KAAKNPGSDA SDPMSFEEYK AFVADYTLEK TAKMSGVPED 350
QLEALAKLYA DPNIKVVSYW TMGFNQHTRG VWANNLCYNL HLLTGKISKP 400
GCGPFSLTGQ PSACGTAREV GTFAHRLPAD MVVTNEKHRQ TAEQKWQLPA 450
GTIPAKVGLH AVAQDRALKD GTLNAYWVMC NNNMQAGPNI NEDRMPGWRD 500
ARNFVVVSDP YPTVSALAAD LILPTAMWVE KEGAYGNAER RTQFWRQQVK 550
APGEAKSDLW QLVEFSKRFT VEEVWPAELL AQKPEYRGKT LYDVLFANGE 600
VNKYPLTEIP ADQLNDEARD FGFYLQKGLF EEYAGFGRGH GHDLAPFDSY 650
HQARGLRWPV VEGKETLWRY REGTDPYVKA GEEVRFYGKP DGKAVIFALP 700
FEPAAESPDK EYDLWLSTGR VLEHWHTGSM TRRVPELHRA FPQAVVFIHP 750
LDAKSRNLRR GEKVRVLSRR GELISTVETR GRNRPPRGLV YMPFFDAAQL 800
VNNLTLDATD PLSKEVDFKK CAVKLEKV 828
Length:828
Mass (Da):92,599
Last modified:November 13, 2007 - v1
Checksum:i46B8C60CD80539E9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000826 Genomic DNA. Translation: ABV42584.1.
RefSeqiYP_001479712.1. NC_009832.1.

Genome annotation databases

EnsemblBacteriaiABV42584; ABV42584; Spro_3486.
GeneIDi5604924.
KEGGispe:Spro_3486.
PATRICi32419776. VBISerPro44537_3545.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000826 Genomic DNA. Translation: ABV42584.1 .
RefSeqi YP_001479712.1. NC_009832.1.

3D structure databases

ProteinModelPortali A8GHJ4.
SMRi A8GHJ4. Positions 37-827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399741.Spro_3486.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV42584 ; ABV42584 ; Spro_3486 .
GeneIDi 5604924.
KEGGi spe:Spro_3486.
PATRICi 32419776. VBISerPro44537_3545.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi HPKTRVA.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci SPRO399741:GI55-3561-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
IPR019546. TAT_signal_bac_arc.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
TIGR01409. TAT_signal_seq. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 568.

Entry informationi

Entry nameiNAPA_SERP5
AccessioniPrimary (citable) accession number: A8GHJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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