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A8GH86

- FADJ_SERP5

UniProt

A8GH86 - FADJ_SERP5

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Protein

Fatty acid oxidation complex subunit alpha

Gene
fadJ, Spro_3378
Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei122 – 1221Important for catalytic activity By similarity
Sitei144 – 1441Important for catalytic activity By similarity

GO - Molecular functioni

  1. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-HAMAP
  2. 3-hydroxybutyryl-CoA epimerase activity Source: UniProtKB-HAMAP
  3. enoyl-CoA hydratase activity Source: UniProtKB-HAMAP
  4. NAD binding Source: InterPro

GO - Biological processi

  1. fatty acid beta-oxidation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase, Oxidoreductase

Keywords - Biological processi

Fatty acid metabolism, Lipid degradation, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciSPRO399741:GI55-3446-MONOMER.
UniPathwayiUPA00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase (EC:4.2.1.17, EC:5.1.2.3)
3-hydroxyacyl-CoA dehydrogenase (EC:1.1.1.35)
Gene namesi
Name:fadJ
Ordered Locus Names:Spro_3378
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
ProteomesiUP000007074: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 715715Fatty acid oxidation complex subunit alphaUniRule annotationPRO_0000335591Add
BLAST

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI) By similarity.

Protein-protein interaction databases

STRINGi399741.Spro_3378.

Structurei

3D structure databases

ProteinModelPortaliA8GH86.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 194194Enoyl-CoA hydratase By similarityAdd
BLAST
Regioni310 – 7154063-hydroxyacyl-CoA dehydrogenase By similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Phylogenomic databases

eggNOGiCOG1250.
HOGENOMiHOG000261346.
KOiK01782.
OrthoDBiEOG6M9F0M.

Family and domain databases

Gene3Di1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPiMF_01617. FadJ.
InterProiIPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsiTIGR02440. FadJ. 1 hit.
PROSITEiPS00067. 3HCDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GH86-1 [UniParc]FASTAAdd to Basket

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MHEQRAKPSA FQLTIRPDNI GVITIDVPGE KVNTLKAEFV EQVNDVLIRA    50
QQHPALEGLV IVSGKPDSFI AGADITMIAA CTTAKEAETL AKKGQSTLAQ 100
IAAFQVPVVA AIHGACLGGG LELALACHGR VCSLDDKTAL GLPEVQLGLL 150
PGSGGTQRLP RLIGASKALD MILTGKHIRA RQALRLGLVD DAVPQSILLQ 200
TAIERVKQGW QSRRELPWQE RLLNGPLGKS LLFSIVRKKT LAKTHGNYPA 250
AERIIQVVRT GLDSGIASGY EAEARAFGEL AMTPQSAALR SLFFASTALK 300
KERGGNAQPH ALHRIGILGG GLMGGGIACV TATRGGLPVR IKDVNETGIN 350
HALKYSWDVL SKRVRSKRMR PAERQKQMML ISGSTDYSGF DQVDVVIEAV 400
FEDLSLKQQM VAEIEQHAAP HTIFASNTSS LPIGQIAAKA QRPEQVIGLH 450
YFSPVDKMPL VEVIPHATTS EETIATTVAL AHKQGKTAIV VADRAGFYVN 500
RILAPYINEA ARCLLEGEPI ESLDKALVDF GFPVGPITLL DEVGIDVGTK 550
ISPVLVEQLG PRFAAPAAFD AVLKDGRKGR KNGRGFYLYP SEGQQRQRHK 600
RADTSVYILL GITPKSHLQQ AVIAQRCVMM MLNEAARCLD EGVIRSARDG 650
DIGAVFGIGF PPFLGGPFRY MDQLGVEKVV KTLEYLQRQH GEYFAPCERL 700
QRMAQQGERF YPQGS 715
Length:715
Mass (Da):77,439
Last modified:May 20, 2008 - v2
Checksum:iA39FA88CFF8406A5
GO

Sequence cautioni

The sequence ABV42476.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000826 Genomic DNA. Translation: ABV42476.1. Different initiation.
RefSeqiYP_001479604.1. NC_009832.1.

Genome annotation databases

EnsemblBacteriaiABV42476; ABV42476; Spro_3378.
GeneIDi5603340.
KEGGispe:Spro_3378.
PATRICi32419542. VBISerPro44537_3435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000826 Genomic DNA. Translation: ABV42476.1 . Different initiation.
RefSeqi YP_001479604.1. NC_009832.1.

3D structure databases

ProteinModelPortali A8GH86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399741.Spro_3378.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV42476 ; ABV42476 ; Spro_3378 .
GeneIDi 5603340.
KEGGi spe:Spro_3378.
PATRICi 32419542. VBISerPro44537_3435.

Phylogenomic databases

eggNOGi COG1250.
HOGENOMi HOG000261346.
KOi K01782.
OrthoDBi EOG6M9F0M.

Enzyme and pathway databases

UniPathwayi UPA00659 .
BioCyci SPRO399741:GI55-3446-MONOMER.

Family and domain databases

Gene3Di 1.10.1040.10. 2 hits.
3.40.50.720. 1 hit.
3.90.226.10. 1 hit.
HAMAPi MF_01617. FadJ.
InterProi IPR006180. 3-OHacyl-CoA_DH_CS.
IPR006176. 3-OHacyl-CoA_DH_NAD-bd.
IPR006108. 3HC_DH_C.
IPR008927. 6-PGluconate_DH_C-like.
IPR029045. ClpP/crotonase-like_dom.
IPR001753. Crotonase_core_superfam.
IPR013328. DH_multihelical.
IPR012802. FadJ.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00725. 3HCDH. 2 hits.
PF02737. 3HCDH_N. 1 hit.
PF00378. ECH. 1 hit.
[Graphical view ]
SUPFAMi SSF48179. SSF48179. 2 hits.
SSF52096. SSF52096. 1 hit.
TIGRFAMsi TIGR02440. FadJ. 1 hit.
PROSITEi PS00067. 3HCDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 568.

Entry informationi

Entry nameiFADJ_SERP5
AccessioniPrimary (citable) accession number: A8GH86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: June 11, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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