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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei122Important for catalytic activityUniRule annotation1
Sitei144Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:Spro_3378
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeSerratia
Proteomesi
  • UP000007074 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003355911 – 715Fatty acid oxidation complex subunit alphaAdd BLAST715

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi399741.Spro_3378

Structurei

3D structure databases

ProteinModelPortaliA8GH86
SMRiA8GH86
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 194Enoyl-CoA hydrataseUniRule annotationAdd BLAST194
Regioni310 – 7153-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST406

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261346
KOiK01782
OrthoDBiPOG091H00UW

Family and domain databases

HAMAPiMF_01617 FadJ, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012802 FadJ
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02440 FadJ, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

A8GH86-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHEQRAKPSA FQLTIRPDNI GVITIDVPGE KVNTLKAEFV EQVNDVLIRA
60 70 80 90 100
QQHPALEGLV IVSGKPDSFI AGADITMIAA CTTAKEAETL AKKGQSTLAQ
110 120 130 140 150
IAAFQVPVVA AIHGACLGGG LELALACHGR VCSLDDKTAL GLPEVQLGLL
160 170 180 190 200
PGSGGTQRLP RLIGASKALD MILTGKHIRA RQALRLGLVD DAVPQSILLQ
210 220 230 240 250
TAIERVKQGW QSRRELPWQE RLLNGPLGKS LLFSIVRKKT LAKTHGNYPA
260 270 280 290 300
AERIIQVVRT GLDSGIASGY EAEARAFGEL AMTPQSAALR SLFFASTALK
310 320 330 340 350
KERGGNAQPH ALHRIGILGG GLMGGGIACV TATRGGLPVR IKDVNETGIN
360 370 380 390 400
HALKYSWDVL SKRVRSKRMR PAERQKQMML ISGSTDYSGF DQVDVVIEAV
410 420 430 440 450
FEDLSLKQQM VAEIEQHAAP HTIFASNTSS LPIGQIAAKA QRPEQVIGLH
460 470 480 490 500
YFSPVDKMPL VEVIPHATTS EETIATTVAL AHKQGKTAIV VADRAGFYVN
510 520 530 540 550
RILAPYINEA ARCLLEGEPI ESLDKALVDF GFPVGPITLL DEVGIDVGTK
560 570 580 590 600
ISPVLVEQLG PRFAAPAAFD AVLKDGRKGR KNGRGFYLYP SEGQQRQRHK
610 620 630 640 650
RADTSVYILL GITPKSHLQQ AVIAQRCVMM MLNEAARCLD EGVIRSARDG
660 670 680 690 700
DIGAVFGIGF PPFLGGPFRY MDQLGVEKVV KTLEYLQRQH GEYFAPCERL
710
QRMAQQGERF YPQGS
Length:715
Mass (Da):77,439
Last modified:May 20, 2008 - v2
Checksum:iA39FA88CFF8406A5
GO

Sequence cautioni

The sequence ABV42476 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA Translation: ABV42476.1 Different initiation.

Genome annotation databases

EnsemblBacteriaiABV42476; ABV42476; Spro_3378
KEGGispe:Spro_3378

Similar proteinsi

Entry informationi

Entry nameiFADJ_SERP5
AccessioniPrimary (citable) accession number: A8GH86
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: May 20, 2008
Last modified: March 28, 2018
This is version 79 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health