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Protein

Beta-galactosidase

Gene

lacZ

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104SubstrateUniRule annotation1
Metal bindingi203SodiumUniRule annotation1
Binding sitei203SubstrateUniRule annotation1
Sitei357Transition state stabilizerUniRule annotation1
Sitei391Transition state stabilizerUniRule annotation1
Metal bindingi416Magnesium 1UniRule annotation1
Metal bindingi418Magnesium 1UniRule annotation1
Active sitei461Proton donorUniRule annotation1
Metal bindingi461Magnesium 1UniRule annotation1
Binding sitei461SubstrateUniRule annotation1
Active sitei537NucleophileUniRule annotation1
Metal bindingi597Magnesium 2UniRule annotation1
Metal bindingi601Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi604SodiumUniRule annotation1
Binding sitei604SubstrateUniRule annotation1
Binding sitei1002SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandMagnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2 Glycoside Hydrolase Family 2

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:Spro_3173
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeSerratia
Proteomesi
  • UP000007074 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003670081 – 1029Beta-galactosidaseAdd BLAST1029

Proteomic databases

PRIDEiA8GGN3

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi399741.Spro_3173

Structurei

3D structure databases

ProteinModelPortaliA8GGN3
SMRiA8GGN3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni537 – 540Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT Bacteria
COG3250 LUCA
HOGENOMiHOG000252443
KOiK01190
OMAiPSNWQLQ
OrthoDBiPOG091H07QB

Family and domain databases

Gene3Di2.60.120.260, 1 hit
2.60.40.10, 2 hits
2.70.98.10, 1 hit
HAMAPiMF_01687 Beta_gal, 1 hit
InterProiView protein in InterPro
IPR004199 B-gal_small/dom_5
IPR036156 Beta-gal/glucu_dom_sf
IPR011013 Gal_mutarotase_sf_dom
IPR008979 Galactose-bd-like_sf
IPR014718 GH-type_carb-bd
IPR006101 Glyco_hydro_2
IPR023232 Glyco_hydro_2_AS
IPR023933 Glyco_hydro_2_beta_Galsidase
IPR006103 Glyco_hydro_2_cat
IPR023230 Glyco_hydro_2_CS
IPR006102 Glyco_hydro_2_Ig-like
IPR006104 Glyco_hydro_2_N
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR032312 LacZ_4
PANTHERiPTHR10066:SF76 PTHR10066:SF76, 1 hit
PfamiView protein in Pfam
PF02929 Bgal_small_N, 1 hit
PF16353 DUF4981, 1 hit
PF00703 Glyco_hydro_2, 1 hit
PF02836 Glyco_hydro_2_C, 1 hit
PF02837 Glyco_hydro_2_N, 1 hit
PRINTSiPR00132 GLHYDRLASE2
SMARTiView protein in SMART
SM01038 Bgal_small_N, 1 hit
SUPFAMiSSF49303 SSF49303, 2 hits
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
SSF74650 SSF74650, 1 hit
PROSITEiView protein in PROSITE
PS00719 GLYCOSYL_HYDROL_F2_1, 1 hit
PS00608 GLYCOSYL_HYDROL_F2_2, 1 hit

Sequencei

Sequence statusi: Complete.

A8GGN3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLPYPSLK DLLARRDWQN PACTHYQRLA AHPPFSSWRN LNAARDDKSS
60 70 80 90 100
ESRQILNGDW QFSYFDKPQA VPDGWLQQDL TDADTLAVPS NWQLAAYDAP
110 120 130 140 150
IYTNVRYPIP VNPPQVPEEN PTGCYSRQFT VDPAWLAEGQ TRIIFDGVNS
160 170 180 190 200
AFYLWCNGHW VGYSQDSRLP AEFDLSPWLQ AGENRLAVMV LRWCDGSYLE
210 220 230 240 250
DQDMWRMSGI FRDVSLLHKP ATHLSDIRIT TPLYDSFRRG ELVAEVHINQ
260 270 280 290 300
PAQHRVQLQL WRDGQLVGEK TQAFGSEIID ERGAYEDRTT LCLPVEQPAL
310 320 330 340 350
WSAETPTLYR ATVTLLSPEG KIIEVEAYDV GFRQVEISNG LLKLNGQPLL
360 370 380 390 400
IRGTNRHEHH PQHGQVMDEA TMRHDILLMK QHNFNAVRCS HYPNHPLWYR
410 420 430 440 450
LCDRYGLYVV DEANIETHGM QPMNRLSDDP LWLPAMSERV TRMVQRDRNH
460 470 480 490 500
PCIIIWSLGN ESGHGCNHDA LYRWVKTQDP TRPVQYEGGG ANSAATDIIC
510 520 530 540 550
PMYARVDQDQ PFPAVPKWSI KKWIGLPDEH RPLILCEYAH AMGNSFGGFD
560 570 580 590 600
RYWQAFRQYP RLQGGFVWDW VDQALTRSDE NGNPYWAYGG DFGDTPNDRQ
610 620 630 640 650
FCLNGLVFPD RTPHPALFEA QRAQQFFQFT FDAETLTLTV NSEYLFRQTD
660 670 680 690 700
NERLNWRLEL DGTERASGSF DLNLLPQSSA SFPLLERLPM LHQPGELWLN
710 720 730 740 750
VEVVQPLATD WSEANHRCAW DQWLVPRTLH FAPPAVAGSA PQLSQNDQTI
760 770 780 790 800
DITHGHQRWQ FTRHDGCLSQ WWQHDHSQLL TPLRDNFIRA PLDNDIGISE
810 820 830 840 850
VERIDPNAWV ERWKLAGMYR LEERCTLLQA DQLSDGVRVV SEHLFEADGQ
860 870 880 890 900
TLLRSRKQWL FDSEGAVSIS VDVDIAASLP PPARIGLSCQ LKEIHPQAQW
910 920 930 940 950
LGLGPHENYP DRRLAAQFGR WQQPLEALHT PYIFPGENGL RCETRSLLYG
960 970 980 990 1000
GWHIDGRFHF SLSRYGLRQL MECSHQHLLQ PEAGTWLSLD GFHMGVGGDD
1010 1020
SWSPSVNQDY LLSGSHYHYQ LRLKRAERS
Length:1,029
Mass (Da):118,303
Last modified:November 13, 2007 - v1
Checksum:i9FFADB850DE58679
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA Translation: ABV42273.1
RefSeqiWP_012145891.1, NC_009832.1

Genome annotation databases

EnsemblBacteriaiABV42273; ABV42273; Spro_3173
KEGGispe:Spro_3173

Similar proteinsi

Entry informationi

Entry nameiBGAL_SERP5
AccessioniPrimary (citable) accession number: A8GGN3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 13, 2007
Last modified: March 28, 2018
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

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