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A8GGN3

- BGAL_SERP5

UniProt

A8GGN3 - BGAL_SERP5

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei104 – 1041SubstrateUniRule annotation
Metal bindingi203 – 2031SodiumUniRule annotation
Binding sitei203 – 2031SubstrateUniRule annotation
Sitei357 – 3571Transition state stabilizerUniRule annotation
Sitei391 – 3911Transition state stabilizerUniRule annotation
Metal bindingi416 – 4161Magnesium 1UniRule annotation
Metal bindingi418 – 4181Magnesium 1UniRule annotation
Active sitei461 – 4611Proton donorUniRule annotation
Metal bindingi461 – 4611Magnesium 1UniRule annotation
Binding sitei461 – 4611SubstrateUniRule annotation
Active sitei537 – 5371NucleophileUniRule annotation
Metal bindingi597 – 5971Magnesium 2UniRule annotation
Metal bindingi601 – 6011Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi604 – 6041SodiumUniRule annotation
Binding sitei604 – 6041SubstrateUniRule annotation
Binding sitei1002 – 10021SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciSPRO399741:GI55-3237-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:Spro_3173
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
ProteomesiUP000007074: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10291029Beta-galactosidasePRO_0000367008Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi399741.Spro_3173.

Structurei

3D structure databases

ProteinModelPortaliA8GGN3.
SMRiA8GGN3. Positions 15-1021.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni537 – 5404Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiNPPFVPK.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GGN3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSLPYPSLK DLLARRDWQN PACTHYQRLA AHPPFSSWRN LNAARDDKSS
60 70 80 90 100
ESRQILNGDW QFSYFDKPQA VPDGWLQQDL TDADTLAVPS NWQLAAYDAP
110 120 130 140 150
IYTNVRYPIP VNPPQVPEEN PTGCYSRQFT VDPAWLAEGQ TRIIFDGVNS
160 170 180 190 200
AFYLWCNGHW VGYSQDSRLP AEFDLSPWLQ AGENRLAVMV LRWCDGSYLE
210 220 230 240 250
DQDMWRMSGI FRDVSLLHKP ATHLSDIRIT TPLYDSFRRG ELVAEVHINQ
260 270 280 290 300
PAQHRVQLQL WRDGQLVGEK TQAFGSEIID ERGAYEDRTT LCLPVEQPAL
310 320 330 340 350
WSAETPTLYR ATVTLLSPEG KIIEVEAYDV GFRQVEISNG LLKLNGQPLL
360 370 380 390 400
IRGTNRHEHH PQHGQVMDEA TMRHDILLMK QHNFNAVRCS HYPNHPLWYR
410 420 430 440 450
LCDRYGLYVV DEANIETHGM QPMNRLSDDP LWLPAMSERV TRMVQRDRNH
460 470 480 490 500
PCIIIWSLGN ESGHGCNHDA LYRWVKTQDP TRPVQYEGGG ANSAATDIIC
510 520 530 540 550
PMYARVDQDQ PFPAVPKWSI KKWIGLPDEH RPLILCEYAH AMGNSFGGFD
560 570 580 590 600
RYWQAFRQYP RLQGGFVWDW VDQALTRSDE NGNPYWAYGG DFGDTPNDRQ
610 620 630 640 650
FCLNGLVFPD RTPHPALFEA QRAQQFFQFT FDAETLTLTV NSEYLFRQTD
660 670 680 690 700
NERLNWRLEL DGTERASGSF DLNLLPQSSA SFPLLERLPM LHQPGELWLN
710 720 730 740 750
VEVVQPLATD WSEANHRCAW DQWLVPRTLH FAPPAVAGSA PQLSQNDQTI
760 770 780 790 800
DITHGHQRWQ FTRHDGCLSQ WWQHDHSQLL TPLRDNFIRA PLDNDIGISE
810 820 830 840 850
VERIDPNAWV ERWKLAGMYR LEERCTLLQA DQLSDGVRVV SEHLFEADGQ
860 870 880 890 900
TLLRSRKQWL FDSEGAVSIS VDVDIAASLP PPARIGLSCQ LKEIHPQAQW
910 920 930 940 950
LGLGPHENYP DRRLAAQFGR WQQPLEALHT PYIFPGENGL RCETRSLLYG
960 970 980 990 1000
GWHIDGRFHF SLSRYGLRQL MECSHQHLLQ PEAGTWLSLD GFHMGVGGDD
1010 1020
SWSPSVNQDY LLSGSHYHYQ LRLKRAERS
Length:1,029
Mass (Da):118,303
Last modified:November 13, 2007 - v1
Checksum:i9FFADB850DE58679
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV42273.1.
RefSeqiYP_001479401.1. NC_009832.1.

Genome annotation databases

EnsemblBacteriaiABV42273; ABV42273; Spro_3173.
GeneIDi5605502.
KEGGispe:Spro_3173.
PATRICi32419128. VBISerPro44537_3233.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV42273.1 .
RefSeqi YP_001479401.1. NC_009832.1.

3D structure databases

ProteinModelPortali A8GGN3.
SMRi A8GGN3. Positions 15-1021.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399741.Spro_3173.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV42273 ; ABV42273 ; Spro_3173 .
GeneIDi 5605502.
KEGGi spe:Spro_3173.
PATRICi 32419128. VBISerPro44537_3233.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi NPPFVPK.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci SPRO399741:GI55-3237-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 568.

Entry informationi

Entry nameiBGAL_SERP5
AccessioniPrimary (citable) accession number: A8GGN3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 13, 2007
Last modified: November 26, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3