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A8GGN3

- BGAL_SERP5

UniProt

A8GGN3 - BGAL_SERP5

Protein

Beta-galactosidase

Gene

lacZ

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei104 – 1041SubstrateUniRule annotation
    Metal bindingi203 – 2031SodiumUniRule annotation
    Binding sitei203 – 2031SubstrateUniRule annotation
    Sitei357 – 3571Transition state stabilizerUniRule annotation
    Sitei391 – 3911Transition state stabilizerUniRule annotation
    Metal bindingi416 – 4161Magnesium 1UniRule annotation
    Metal bindingi418 – 4181Magnesium 1UniRule annotation
    Active sitei461 – 4611Proton donorUniRule annotation
    Metal bindingi461 – 4611Magnesium 1UniRule annotation
    Binding sitei461 – 4611SubstrateUniRule annotation
    Active sitei537 – 5371NucleophileUniRule annotation
    Metal bindingi597 – 5971Magnesium 2UniRule annotation
    Metal bindingi601 – 6011Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi604 – 6041SodiumUniRule annotation
    Binding sitei604 – 6041SubstrateUniRule annotation
    Binding sitei1002 – 10021SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciSPRO399741:GI55-3237-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:Spro_3173
    OrganismiSerratia proteamaculans (strain 568)
    Taxonomic identifieri399741 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
    ProteomesiUP000007074: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10291029Beta-galactosidasePRO_0000367008Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi399741.Spro_3173.

    Structurei

    3D structure databases

    ProteinModelPortaliA8GGN3.
    SMRiA8GGN3. Positions 15-1021.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni537 – 5404Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiNPPFVPK.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8GGN3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLPYPSLK DLLARRDWQN PACTHYQRLA AHPPFSSWRN LNAARDDKSS     50
    ESRQILNGDW QFSYFDKPQA VPDGWLQQDL TDADTLAVPS NWQLAAYDAP 100
    IYTNVRYPIP VNPPQVPEEN PTGCYSRQFT VDPAWLAEGQ TRIIFDGVNS 150
    AFYLWCNGHW VGYSQDSRLP AEFDLSPWLQ AGENRLAVMV LRWCDGSYLE 200
    DQDMWRMSGI FRDVSLLHKP ATHLSDIRIT TPLYDSFRRG ELVAEVHINQ 250
    PAQHRVQLQL WRDGQLVGEK TQAFGSEIID ERGAYEDRTT LCLPVEQPAL 300
    WSAETPTLYR ATVTLLSPEG KIIEVEAYDV GFRQVEISNG LLKLNGQPLL 350
    IRGTNRHEHH PQHGQVMDEA TMRHDILLMK QHNFNAVRCS HYPNHPLWYR 400
    LCDRYGLYVV DEANIETHGM QPMNRLSDDP LWLPAMSERV TRMVQRDRNH 450
    PCIIIWSLGN ESGHGCNHDA LYRWVKTQDP TRPVQYEGGG ANSAATDIIC 500
    PMYARVDQDQ PFPAVPKWSI KKWIGLPDEH RPLILCEYAH AMGNSFGGFD 550
    RYWQAFRQYP RLQGGFVWDW VDQALTRSDE NGNPYWAYGG DFGDTPNDRQ 600
    FCLNGLVFPD RTPHPALFEA QRAQQFFQFT FDAETLTLTV NSEYLFRQTD 650
    NERLNWRLEL DGTERASGSF DLNLLPQSSA SFPLLERLPM LHQPGELWLN 700
    VEVVQPLATD WSEANHRCAW DQWLVPRTLH FAPPAVAGSA PQLSQNDQTI 750
    DITHGHQRWQ FTRHDGCLSQ WWQHDHSQLL TPLRDNFIRA PLDNDIGISE 800
    VERIDPNAWV ERWKLAGMYR LEERCTLLQA DQLSDGVRVV SEHLFEADGQ 850
    TLLRSRKQWL FDSEGAVSIS VDVDIAASLP PPARIGLSCQ LKEIHPQAQW 900
    LGLGPHENYP DRRLAAQFGR WQQPLEALHT PYIFPGENGL RCETRSLLYG 950
    GWHIDGRFHF SLSRYGLRQL MECSHQHLLQ PEAGTWLSLD GFHMGVGGDD 1000
    SWSPSVNQDY LLSGSHYHYQ LRLKRAERS 1029
    Length:1,029
    Mass (Da):118,303
    Last modified:November 13, 2007 - v1
    Checksum:i9FFADB850DE58679
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000826 Genomic DNA. Translation: ABV42273.1.
    RefSeqiYP_001479401.1. NC_009832.1.

    Genome annotation databases

    EnsemblBacteriaiABV42273; ABV42273; Spro_3173.
    GeneIDi5605502.
    KEGGispe:Spro_3173.
    PATRICi32419128. VBISerPro44537_3233.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000826 Genomic DNA. Translation: ABV42273.1 .
    RefSeqi YP_001479401.1. NC_009832.1.

    3D structure databases

    ProteinModelPortali A8GGN3.
    SMRi A8GGN3. Positions 15-1021.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399741.Spro_3173.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV42273 ; ABV42273 ; Spro_3173 .
    GeneIDi 5605502.
    KEGGi spe:Spro_3173.
    PATRICi 32419128. VBISerPro44537_3233.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi NPPFVPK.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci SPRO399741:GI55-3237-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 568.

    Entry informationi

    Entry nameiBGAL_SERP5
    AccessioniPrimary (citable) accession number: A8GGN3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3