Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A8GGN3 (BGAL_SERP5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:Spro_3173
OrganismSerratia proteamaculans (strain 568) [Complete proteome] [HAMAP]
Taxonomic identifier399741 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Protein attributes

Sequence length1029 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10291029Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000367008

Regions

Region537 – 5404Substrate binding By similarity

Sites

Active site4611Proton donor By similarity
Active site5371Nucleophile By similarity
Metal binding2031Sodium By similarity
Metal binding4161Magnesium 1 By similarity
Metal binding4181Magnesium 1 By similarity
Metal binding4611Magnesium 1 By similarity
Metal binding5971Magnesium 2 By similarity
Metal binding6011Sodium; via carbonyl oxygen By similarity
Metal binding6041Sodium By similarity
Binding site1041Substrate By similarity
Binding site2031Substrate By similarity
Binding site4611Substrate By similarity
Binding site6041Substrate By similarity
Binding site10021Substrate By similarity
Site3571Transition state stabilizer By similarity
Site3911Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A8GGN3 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: 9FFADB850DE58679

FASTA1,029118,303
        10         20         30         40         50         60 
MSSLPYPSLK DLLARRDWQN PACTHYQRLA AHPPFSSWRN LNAARDDKSS ESRQILNGDW 

        70         80         90        100        110        120 
QFSYFDKPQA VPDGWLQQDL TDADTLAVPS NWQLAAYDAP IYTNVRYPIP VNPPQVPEEN 

       130        140        150        160        170        180 
PTGCYSRQFT VDPAWLAEGQ TRIIFDGVNS AFYLWCNGHW VGYSQDSRLP AEFDLSPWLQ 

       190        200        210        220        230        240 
AGENRLAVMV LRWCDGSYLE DQDMWRMSGI FRDVSLLHKP ATHLSDIRIT TPLYDSFRRG 

       250        260        270        280        290        300 
ELVAEVHINQ PAQHRVQLQL WRDGQLVGEK TQAFGSEIID ERGAYEDRTT LCLPVEQPAL 

       310        320        330        340        350        360 
WSAETPTLYR ATVTLLSPEG KIIEVEAYDV GFRQVEISNG LLKLNGQPLL IRGTNRHEHH 

       370        380        390        400        410        420 
PQHGQVMDEA TMRHDILLMK QHNFNAVRCS HYPNHPLWYR LCDRYGLYVV DEANIETHGM 

       430        440        450        460        470        480 
QPMNRLSDDP LWLPAMSERV TRMVQRDRNH PCIIIWSLGN ESGHGCNHDA LYRWVKTQDP 

       490        500        510        520        530        540 
TRPVQYEGGG ANSAATDIIC PMYARVDQDQ PFPAVPKWSI KKWIGLPDEH RPLILCEYAH 

       550        560        570        580        590        600 
AMGNSFGGFD RYWQAFRQYP RLQGGFVWDW VDQALTRSDE NGNPYWAYGG DFGDTPNDRQ 

       610        620        630        640        650        660 
FCLNGLVFPD RTPHPALFEA QRAQQFFQFT FDAETLTLTV NSEYLFRQTD NERLNWRLEL 

       670        680        690        700        710        720 
DGTERASGSF DLNLLPQSSA SFPLLERLPM LHQPGELWLN VEVVQPLATD WSEANHRCAW 

       730        740        750        760        770        780 
DQWLVPRTLH FAPPAVAGSA PQLSQNDQTI DITHGHQRWQ FTRHDGCLSQ WWQHDHSQLL 

       790        800        810        820        830        840 
TPLRDNFIRA PLDNDIGISE VERIDPNAWV ERWKLAGMYR LEERCTLLQA DQLSDGVRVV 

       850        860        870        880        890        900 
SEHLFEADGQ TLLRSRKQWL FDSEGAVSIS VDVDIAASLP PPARIGLSCQ LKEIHPQAQW 

       910        920        930        940        950        960 
LGLGPHENYP DRRLAAQFGR WQQPLEALHT PYIFPGENGL RCETRSLLYG GWHIDGRFHF 

       970        980        990       1000       1010       1020 
SLSRYGLRQL MECSHQHLLQ PEAGTWLSLD GFHMGVGGDD SWSPSVNQDY LLSGSHYHYQ 


LRLKRAERS 

« Hide

References

[1]"Complete sequence of chromosome of Serratia proteamaculans 568."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. expand/collapse author list , Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 568.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000826 Genomic DNA. Translation: ABV42273.1.
RefSeqYP_001479401.1. NC_009832.1.

3D structure databases

ProteinModelPortalA8GGN3.
SMRA8GGN3. Positions 15-1021.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING399741.Spro_3173.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV42273; ABV42273; Spro_3173.
GeneID5605502.
KEGGspe:Spro_3173.
PATRIC32419128. VBISerPro44537_3233.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMANPPFVPK.
OrthoDBEOG6XWV0T.

Enzyme and pathway databases

BioCycSPRO399741:GI55-3237-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_SERP5
AccessionPrimary (citable) accession number: A8GGN3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries