Reviewed, UniProtKB/Swiss-Prot A8GG83 (T23O_SERP5)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase
      Short name=TDO
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase
      Short name=Tryptophanase
    Tryptophan oxygenase
      Short name=TRPO
      Short name=TO
    Tryptamin 2,3-dioxygenase

Gene names

Name:	kynA
Ordered Locus Names:	Spro_3022

Organism

Serratia proteamaculans (strain 568) [Complete proteome] [HAMAP]

Taxonomic identifier

399741 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	Tryptophan 2,3-dioxygenase		PRO_0000360133
Regions
	Region	20 – 24	5	Substrate binding By similarity
	Region	47 – 51	5	Substrate binding By similarity
Sites
	Metal binding	236	1	Iron (heme axial ligand) By similarity
	Binding site	109	1	Substrate By similarity
	Binding site	113	1	Substrate By similarity
	Binding site	120	1	Heme By similarity
	Binding site	250	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GG83-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: FCDB8E50CC4A8978	FASTA	280	32,713
	10 20 30 40 50 60 MNKRELESAI VTDFSKRMSY GDYLCLDQLL DCQHPLSNPQ HHDEMLFVVQ HQTSELWMKL 70 80 90 100 110 120 MLHELQAARI LVQQDKLSHC FKILARVKQI QRLLFEQWAV LETLTPSEYV EFRDVLGNSS 130 140 150 160 170 180 GFQSHQYRSI EFLLGNKNAA MLAVFSNDAD KHAALKAILE APSLYDEYLL YLSRHGLPIP 190 200 210 220 230 240 QECIERDWTQ PYQRNPDLLP AFKEIYDHPQ KYWEAYEMAE KLVDIEESFH LWRFRHMKTV 250 260 270 280 ERIIGFKTGT GGSSGVSFLK KALELTFFPE LLDVRTEIGA

References

[1]

"Complete sequence of chromosome of Serratia proteamaculans 568." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. , Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000826 Genomic DNA. Translation: ABV42123.1.
RefSeq	YP_001479251.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8GG83.
Genome annotation databases
GeneID	5604053.
GenomeReviews	Gene locus Spro_3022 in contig CP000826_GR.
KEGG	spe:Spro_3022.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QWSVLAT.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_SERP5

Accession

Primary (citable) accession number: A8GG83

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	November 13, 2007
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)