Reviewed, UniProtKB/Swiss-Prot A8GD46 (GHRA_SERP5)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase A
    EC=1.1.1.79
    EC=1.1.1.81
Alternative name(s):
    2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	Spro_1933

Organism

Serratia proteamaculans (strain 568) [Complete proteome] [HAMAP]

Taxonomic identifier

399741 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity.
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01666
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666		PRO_0000348373
Sites
	Active site	228	1	By similarity
	Active site	276	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8GD46-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: EDEDACA97CA14D1C	FASTA	313	34,642
	10 20 30 40 50 60 MNIIFYHPFF NAEEWLAGLS QRLPQAQVRA WQPGDDRPAD YALVWRPPHE MLANRGRLKG 70 80 90 100 110 120 VFALGAGVDA ILEQERAYPG TLPAGVPLLR LEDTGMAQQM QEYALSYVLR YFRRFDEYQL 130 140 150 160 170 180 LQQQQEWRPL DPHQQDNFTI GILGAGVLGK SVAQKLTAFG FNVRCWSRSP KQIDGVQSFA 190 200 210 220 230 240 GAEQRAAFLD GVQLLINLLP NTPETAGILN QQLFAHLAPG AYLINLARGA HLVEDDLLQA 250 260 270 280 290 300 LEQGQLAAAT LDVFVTEPLP QAHPFWRHPR VTITPHIAAI TLPQAAMDQI AANILALEAG 310 QTPAGVVDVL LGY

References

[1]

"Complete sequence of chromosome of Serratia proteamaculans 568." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. , Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000826 Genomic DNA. Translation: ABV41036.1.
RefSeq	YP_001478164.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5606872.
GenomeReviews	Gene locus Spro_1933 in contig CP000826_GR.
KEGG	spe:Spro_1933.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8GD46. YALVWRA.
Family and domain databases
HAMAP	MF_01666. [Tree]
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_SERP5

Accession

Primary (citable) accession number: A8GD46

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)