Skip Header

Contribute Send feedback
Read comments (?) or add your own

A8GC31 (MAO1_SERP5) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 34. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD-dependent malic enzyme
Short name=NAD-ME
EC=1.1.1.38
Gene names
Name:maeA
Ordered Locus Names:Spro_1567
OrganismSerratia proteamaculans (strain 568) [Complete proteome] [HAMAP]
Taxonomic identifier399741 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length565 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = pyruvate + CO2 + NADH. HAMAP MF_01619

Cofactor

Divalent metal cations. Prefers magnesium or manganese By similarity.

Subunit structure

Homotetramer By similarity. HAMAP MF_01619

Sequence similarities

Belongs to the malic enzymes family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 565565NAD-dependent malic enzyme HAMAP MF_01619
PRO_1000069537

Sites

Active site1041Proton donor By similarity
Active site1751Proton acceptor By similarity
Metal binding2461Divalent metal cation By similarity
Metal binding2471Divalent metal cation By similarity
Metal binding2701Divalent metal cation By similarity
Binding site1571NAD By similarity
Binding site2701NAD By similarity
Binding site4181NAD By similarity
Site2701Important for activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A8GC31 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: ACD380A89DEABC20

FASTA56562,750
        10         20         30         40         50         60 
MELEYESKRP LYIPYAGPIL LEFPLLNKGS AFTEEERNHF NLQGLLPDAV ETIEEQAERA 

        70         80         90        100        110        120 
YRQYQDFKND SDKHIYLRNI QDTNETLFYR LLDSHLSEMM PIIYTPTVGE ACEHFSDIYR 

       130        140        150        160        170        180 
RARGLFISYP NRDRIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT 

       190        200        210        220        230        240 
ACGGISPAYT LPVVLDVGTN NPQRLNDPLY MGWRHPRISG DEYHAFVEEF IQAVKRRWPN 

       250        260        270        280        290        300 
VLLQFEDFAQ NNATPLLNRY RDEICCFNDD IQGTAAVTLG SLIAASRAAG SQLRDQTVTF 

       310        320        330        340        350        360 
LGAGSAGCGI AEQIIAQMKS EGLSEDEARA RVFMVDRFGL LTDKLPNLLD FQSKLVQKSE 

       370        380        390        400        410        420 
NLTAWQTQSD AISLLDVVRN AKPTILIGVS GQPGLFTEEL IREMHKHCPR PIVMPLSNPT 

       430        440        450        460        470        480 
SRVEARPEDI INWTEGAALV ATGSPFAPVH YKEQQIPIAQ CNNSYIFPGI GLGVLASGAT 

       490        500        510        520        530        540 
RVTDAMLMAA SRALAECSPL ATDGHGALLP DIDDIQGVSK CIAMEVGKAA QLQGMAVVTS 

       550        560 
EEALSKAIEH NFWRPQYRSY KRTSF

« Hide

References

[1]"Complete sequence of chromosome of Serratia proteamaculans 568."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. expand/collapse author list , Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P.
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 568.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000826 Genomic DNA. Translation: ABV40671.1.
RefSeqYP_001477799.1. NC_009832.1.

3D structure databases

ProteinModelPortalA8GC31.
ModBaseSearch...

Protein-protein interaction databases

STRINGA8GC31.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5602866.
GenomeReviewsGene locus Spro_1567 in contig CP000826_GR.
KEGGspe:Spro_1567.
PATRIC32415826. VBISerPro44537_1598.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0281.
HOGENOMHBG289821.
OMAQDNNETL.
ProtClustDBPRK13529.

Enzyme and pathway databases

BioCycSPRO399741:SPRO_1567-MONOMER.

Family and domain databases

HAMAPMF_01619. NAD_malic_enz.
[Tree]
InterProIPR015884. Malic_enzyme_CS.
IPR012301. Malic_N.
IPR012302. Malic_NAD-bd.
IPR001891. Malic_OxRdtase.
IPR016040. NAD(P)-bd_dom.
IPR023667. NAD_malic_enz_proteobac.
[Graphical view]
Gene3DG3DSA:3.40.50.10380. G3DSA:3.40.50.10380. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00027.
PfamPF00390. malic. 1 hit.
PF03949. Malic_M. 1 hit.
[Graphical view]
PIRSFPIRSF000106. ME. 1 hit.
PRINTSPR00072. MALOXRDTASE.
SMARTSM00919. Malic_M. 1 hit.
[Graphical view]
PROSITEPS00331. MALIC_ENZYMES. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMAO1_SERP5
AccessionPrimary (citable) accession number: A8GC31
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents