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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPRO399741:GI55-1246-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Spro_1207
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
ProteomesiUP000007074: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 860860Leucine--tRNA ligasePRO_1000057348Add
BLAST

Proteomic databases

PRIDEiA8GB21.

Interactioni

Protein-protein interaction databases

STRINGi399741.Spro_1207.

Structurei

3D structure databases

ProteinModelPortaliA8GB21.
SMRiA8GB21. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8GB21-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQEQYRPEDI ESNVQLHWQE KQTFKVTEDA SKEKYYCLSM LPYPSGRLHM
60 70 80 90 100
GHVRNYTIGD VISRYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW
110 120 130 140 150
TYDNIEYMKN QLKLLGFGYD WDREIATCDP DYYRWEQWFF TKLYEKGLVY
160 170 180 190 200
KKTSAVNWCP HDLTVLANEQ VIDGCCWRCD TKVERKEIPQ WFIKITDYAD
210 220 230 240 250
QLLNDLDTLE SWPEQVKTMQ RNWIGRSEGV DITFDVAGSD EKLTVYTTRP
260 270 280 290 300
DTFMGATYVA VAAGHPLAQQ GAVNNPALAE FIEECRNTKV AEAEMATMEK
310 320 330 340 350
KGMPTGLFAI HPLSGEQVPV WVANFVLMEY GTGAVMAVPA HDQRDWEFAT
360 370 380 390 400
KYGLPIKPVV LALDGSQPDV QAEAMTEKGT LFNSGEFDGL DHQDGFNAIA
410 420 430 440 450
DKLVAKGVGQ RKVNYRLRDW GVSRQRYWGA PIPMVTLEDG TVMPTPEDQL
460 470 480 490 500
PVILPEDVVM DGITSPIKAD PEWAKTTVNG QPALRETDTF DTFMESSWYY
510 520 530 540 550
ARYTCPQYDK GMLDPAAANY WLPVDQYVGG IEHAIMHLMY FRFFHKLLRD
560 570 580 590 600
AGLVDSDEPA KRLLCQGMVL ADAFYYSGNS GERVWVSPAD AIVERDDKGR
610 620 630 640 650
IIKATDPQGR ELVYAGMSKM SKSKNNGIDP QVMVEKYGAD TVRLFMMFAS
660 670 680 690 700
PAEMTLEWQE SGVEGANRFL KRVWKLSYEH LEKGAVQPLN VATLTEDQKA
710 720 730 740 750
LRRDLHKTIA KVTDDIGRRQ TFNTAIAAVM ELMNKLARAP QESEQDRALL
760 770 780 790 800
QEALLAVVRM LYPFTPHVCF SLWQALGGEG DVDTAAWPQA DEQAMVEDSK
810 820 830 840 850
LVVVQVNGKV RAKITVAADA TEEQVRARAA EEHLVAKYLD GVTVRKVIYV
860
PGKLLNLVVG
Length:860
Mass (Da):97,024
Last modified:November 13, 2007 - v1
Checksum:iB55556EAA82BE5AE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV40311.1.
RefSeqiYP_001477439.1. NC_009832.1.

Genome annotation databases

EnsemblBacteriaiABV40311; ABV40311; Spro_1207.
GeneIDi5602925.
KEGGispe:Spro_1207.
PATRICi32415090. VBISerPro44537_1241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV40311.1.
RefSeqiYP_001477439.1. NC_009832.1.

3D structure databases

ProteinModelPortaliA8GB21.
SMRiA8GB21. Positions 228-413.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399741.Spro_1207.

Proteomic databases

PRIDEiA8GB21.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV40311; ABV40311; Spro_1207.
GeneIDi5602925.
KEGGispe:Spro_1207.
PATRICi32415090. VBISerPro44537_1241.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciSPRO399741:GI55-1246-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 568.

Entry informationi

Entry nameiSYL_SERP5
AccessioniPrimary (citable) accession number: A8GB21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: January 7, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.