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Protein

5'/3'-nucleotidase SurE

Gene

surE

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain-length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs.UniRule annotation

Catalytic activityi

A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.UniRule annotation
A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate.UniRule annotation
(Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Divalent metal cationUniRule annotation
Metal bindingi9 – 91Divalent metal cationUniRule annotation
Metal bindingi39 – 391Divalent metal cationUniRule annotation
Metal bindingi92 – 921Divalent metal cationUniRule annotation

GO - Molecular functioni

  1. 3'-nucleotidase activity Source: UniProtKB-HAMAP
  2. 5'-nucleotidase activity Source: UniProtKB-HAMAP
  3. exopolyphosphatase activity Source: UniProtKB-HAMAP
  4. metal ion binding Source: UniProtKB-HAMAP
  5. nucleotide binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSPRO399741:GI55-857-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
5'/3'-nucleotidase SurEUniRule annotation (EC:3.1.3.5UniRule annotation, EC:3.1.3.6UniRule annotation)
Alternative name(s):
ExopolyphosphataseUniRule annotation (EC:3.6.1.11UniRule annotation)
Nucleoside monophosphate phosphohydrolaseUniRule annotation
Gene namesi
Name:surEUniRule annotation
Ordered Locus Names:Spro_0829
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
ProteomesiUP000007074: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2532535'/3'-nucleotidase SurEPRO_0000335269Add
BLAST

Proteomic databases

PRIDEiA8G9Z5.

Interactioni

Protein-protein interaction databases

STRINGi399741.Spro_0829.

Structurei

3D structure databases

ProteinModelPortaliA8G9Z5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SurE nucleotidase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0496.
HOGENOMiHOG000122500.
KOiK03787.
OMAiENWLASM.
OrthoDBiEOG68WR45.

Family and domain databases

Gene3Di3.40.1210.10. 1 hit.
HAMAPiMF_00060. SurE.
InterProiIPR030048. SurE.
IPR002828. SurE-like_Pase/nucleotidase.
[Graphical view]
PfamiPF01975. SurE. 1 hit.
[Graphical view]
SUPFAMiSSF64167. SSF64167. 1 hit.
TIGRFAMsiTIGR00087. surE. 1 hit.

Sequencei

Sequence statusi: Complete.

A8G9Z5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRILLSNDDG VTAPGIQVLA AALREFAEVQ VVAPDRNRSG SSNALTLESP
60 70 80 90 100
LRTLTMPNGD IAVQQGTPTD CVYLGVNALM QPAPDIVVSG INAGPNLGDD
110 120 130 140 150
VIYSGTVAAA MEGRHLGLPA LAVSLNGHQH YATAAAITCR VLRALQREPL
160 170 180 190 200
RTGKILNINV PDLPLDQIKG IRVTRCGSRH PADKVFCQQD PRGQNLYWIG
210 220 230 240 250
PPGDKFDAGP DTDFAAVEQG YVAITPLQVD LTAYAAQDVV KTWLTKAGVG

GEW
Length:253
Mass (Da):26,842
Last modified:November 13, 2007 - v1
Checksum:iCC6B502A1B030085
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV39935.1.
RefSeqiYP_001477063.1. NC_009832.1.

Genome annotation databases

EnsemblBacteriaiABV39935; ABV39935; Spro_0829.
GeneIDi5603034.
KEGGispe:Spro_0829.
PATRICi32414302. VBISerPro44537_0858.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV39935.1.
RefSeqiYP_001477063.1. NC_009832.1.

3D structure databases

ProteinModelPortaliA8G9Z5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399741.Spro_0829.

Proteomic databases

PRIDEiA8G9Z5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV39935; ABV39935; Spro_0829.
GeneIDi5603034.
KEGGispe:Spro_0829.
PATRICi32414302. VBISerPro44537_0858.

Phylogenomic databases

eggNOGiCOG0496.
HOGENOMiHOG000122500.
KOiK03787.
OMAiENWLASM.
OrthoDBiEOG68WR45.

Enzyme and pathway databases

BioCyciSPRO399741:GI55-857-MONOMER.

Family and domain databases

Gene3Di3.40.1210.10. 1 hit.
HAMAPiMF_00060. SurE.
InterProiIPR030048. SurE.
IPR002828. SurE-like_Pase/nucleotidase.
[Graphical view]
PfamiPF01975. SurE. 1 hit.
[Graphical view]
SUPFAMiSSF64167. SSF64167. 1 hit.
TIGRFAMsiTIGR00087. surE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 568.

Entry informationi

Entry nameiSURE_SERP5
AccessioniPrimary (citable) accession number: A8G9Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: January 7, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.