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Protein

Siroheme synthase 1

Gene

cysG1

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei228 – 2281S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Active sitei251 – 2511Proton acceptorUniRule annotation
Active sitei273 – 2731Proton donorUniRule annotation
Binding sitei309 – 3091S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei386 – 3861S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
Binding sitei415 – 4151S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NADUniRule annotation
Nucleotide bindingi43 – 442NADUniRule annotation

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSPRO399741:GI55-845-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 1UniRule annotation
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 1UniRule annotation (EC:2.1.1.107UniRule annotation)
Short name:
Urogen III methylase 1UniRule annotation
Alternative name(s):
SUMT 1UniRule annotation
Uroporphyrinogen III methylase 1UniRule annotation
Short name:
UROM 1UniRule annotation
Precorrin-2 dehydrogenase 1UniRule annotation (EC:1.3.1.76UniRule annotation)
Sirohydrochlorin ferrochelatase 1UniRule annotation (EC:4.99.1.4UniRule annotation)
Gene namesi
Name:cysG1UniRule annotation
Ordered Locus Names:Spro_0817
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
ProteomesiUP000007074: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 476476Siroheme synthase 1PRO_0000330557Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281PhosphoserineUniRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi399741.Spro_0817.

Structurei

3D structure databases

ProteinModelPortaliA8G9Y3.
SMRiA8G9Y3. Positions 1-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd
BLAST
Regioni219 – 476258Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd
BLAST
Regioni304 – 3063S-adenosyl-L-methionine bindingUniRule annotation
Regioni334 – 3352S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiLHQQLAW.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8G9Y3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYLPIFADL KQRPVLVVGG GEVAARKVDL LLRAGAEIRI VAQSLSPILE
60 70 80 90 100
QLSQQGQIHW LGQAFAAEQL DEVFLVIAAT DDSALNAEVF SEADKRRVLA
110 120 130 140 150
NVVDDQPRCS FIFPSIIDRS PLVVAVSSSG QAPVLARMLR EKLEALLPAS
160 170 180 190 200
LGQMAEVAGR WRGQVKQRLN AIGERRRFWE KTFGGRFATL VANGQTAEAQ
210 220 230 240 250
RQLEQDLEQF AQGSEGTQGE IALVGAGPGD VGLLTLRGLQ VMQQADVVLY
260 270 280 290 300
DHLVSDEILD LVRRDAERIC VGKRAGAHSV IQEETNRLLV ELAQQGKRVV
310 320 330 340 350
RLKGGDPFIF GRGGEELQVA AAAGIPFQVV PGVTAAAGAT AYAGIPLTHR
360 370 380 390 400
DHAQSVTFIT GHCRPDGDGL DWADLARARQ TLAIYMGTMK AADISQRLIA
410 420 430 440 450
HGRAATTPVA VISRGTRADQ LVQTGTLQQL EQLAQQAPLP ALLVIGEVVE
460 470
LHHQIAWFGH QPQAEGVSRP AVVNLA
Length:476
Mass (Da):51,267
Last modified:November 13, 2007 - v1
Checksum:i2DAB7E09F9A3B2B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV39923.1.
RefSeqiWP_012005265.1. NC_009832.1.
YP_001477051.1. NC_009832.1.

Genome annotation databases

EnsemblBacteriaiABV39923; ABV39923; Spro_0817.
GeneIDi5603444.
KEGGispe:Spro_0817.
PATRICi32414278. VBISerPro44537_0846.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000826 Genomic DNA. Translation: ABV39923.1.
RefSeqiWP_012005265.1. NC_009832.1.
YP_001477051.1. NC_009832.1.

3D structure databases

ProteinModelPortaliA8G9Y3.
SMRiA8G9Y3. Positions 1-460.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi399741.Spro_0817.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV39923; ABV39923; Spro_0817.
GeneIDi5603444.
KEGGispe:Spro_0817.
PATRICi32414278. VBISerPro44537_0846.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiLHQQLAW.
OrthoDBiEOG6DRPFR.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.
BioCyciSPRO399741:GI55-845-MONOMER.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 568.

Entry informationi

Entry nameiCYSG1_SERP5
AccessioniPrimary (citable) accession number: A8G9Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: November 13, 2007
Last modified: February 4, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.