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A8G9Y3

- CYSG1_SERP5

UniProt

A8G9Y3 - CYSG1_SERP5

Protein

Siroheme synthase 1

Gene

cysG1

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
    S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
    Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
    Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei228 – 2281S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei251 – 2511Proton acceptorUniRule annotation
    Active sitei273 – 2731Proton donorUniRule annotation
    Binding sitei309 – 3091S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei386 – 3861S-adenosyl-L-methionine; via amide nitrogenUniRule annotation
    Binding sitei415 – 4151S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi22 – 232NADUniRule annotation
    Nucleotide bindingi43 – 442NADUniRule annotation

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
    3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
    4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
    2. siroheme biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase, Methyltransferase, Oxidoreductase, Transferase

    Keywords - Biological processi

    Cobalamin biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    NAD, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSPRO399741:GI55-845-MONOMER.
    UniPathwayiUPA00148; UER00211.
    UPA00148; UER00222.
    UPA00262; UER00211.
    UPA00262; UER00222.
    UPA00262; UER00376.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Siroheme synthase 1UniRule annotation
    Including the following 3 domains:
    Uroporphyrinogen-III C-methyltransferase 1UniRule annotation (EC:2.1.1.107UniRule annotation)
    Short name:
    Urogen III methylase 1UniRule annotation
    Alternative name(s):
    SUMT 1UniRule annotation
    Uroporphyrinogen III methylase 1UniRule annotation
    Short name:
    UROM 1UniRule annotation
    Precorrin-2 dehydrogenase 1UniRule annotation (EC:1.3.1.76UniRule annotation)
    Sirohydrochlorin ferrochelatase 1UniRule annotation (EC:4.99.1.4UniRule annotation)
    Gene namesi
    Name:cysG1UniRule annotation
    Ordered Locus Names:Spro_0817
    OrganismiSerratia proteamaculans (strain 568)
    Taxonomic identifieri399741 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
    ProteomesiUP000007074: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 476476Siroheme synthase 1PRO_0000330557Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281PhosphoserineUniRule annotation

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi399741.Spro_0817.

    Structurei

    3D structure databases

    ProteinModelPortaliA8G9Y3.
    SMRiA8G9Y3. Positions 1-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelataseUniRule annotationAdd
    BLAST
    Regioni219 – 476258Uroporphyrinogen-III C-methyltransferaseUniRule annotationAdd
    BLAST
    Regioni304 – 3063S-adenosyl-L-methionine bindingUniRule annotation
    Regioni334 – 3352S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase family.UniRule annotation
    In the C-terminal section; belongs to the precorrin methyltransferase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0007.
    HOGENOMiHOG000290518.
    KOiK02302.
    OMAiLHQQLAW.
    OrthoDBiEOG6DRPFR.

    Family and domain databases

    Gene3Di1.10.8.210. 1 hit.
    3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPiMF_01646. Siroheme_synth.
    InterProiIPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR016040. NAD(P)-bd_dom.
    IPR012409. Sirohaem_synth.
    IPR019478. Sirohaem_synthase_dimer_dom.
    IPR006367. Sirohaem_synthase_N.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view]
    PfamiPF10414. CysG_dimeriser. 1 hit.
    PF13241. NAD_binding_7. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
    SUPFAMiSSF53790. SSF53790. 1 hit.
    TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
    TIGR01470. cysG_Nterm. 1 hit.
    PROSITEiPS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8G9Y3-1 [UniParc]FASTAAdd to Basket

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    MDYLPIFADL KQRPVLVVGG GEVAARKVDL LLRAGAEIRI VAQSLSPILE    50
    QLSQQGQIHW LGQAFAAEQL DEVFLVIAAT DDSALNAEVF SEADKRRVLA 100
    NVVDDQPRCS FIFPSIIDRS PLVVAVSSSG QAPVLARMLR EKLEALLPAS 150
    LGQMAEVAGR WRGQVKQRLN AIGERRRFWE KTFGGRFATL VANGQTAEAQ 200
    RQLEQDLEQF AQGSEGTQGE IALVGAGPGD VGLLTLRGLQ VMQQADVVLY 250
    DHLVSDEILD LVRRDAERIC VGKRAGAHSV IQEETNRLLV ELAQQGKRVV 300
    RLKGGDPFIF GRGGEELQVA AAAGIPFQVV PGVTAAAGAT AYAGIPLTHR 350
    DHAQSVTFIT GHCRPDGDGL DWADLARARQ TLAIYMGTMK AADISQRLIA 400
    HGRAATTPVA VISRGTRADQ LVQTGTLQQL EQLAQQAPLP ALLVIGEVVE 450
    LHHQIAWFGH QPQAEGVSRP AVVNLA 476
    Length:476
    Mass (Da):51,267
    Last modified:November 13, 2007 - v1
    Checksum:i2DAB7E09F9A3B2B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000826 Genomic DNA. Translation: ABV39923.1.
    RefSeqiWP_012005265.1. NC_009832.1.
    YP_001477051.1. NC_009832.1.

    Genome annotation databases

    EnsemblBacteriaiABV39923; ABV39923; Spro_0817.
    GeneIDi5603444.
    KEGGispe:Spro_0817.
    PATRICi32414278. VBISerPro44537_0846.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000826 Genomic DNA. Translation: ABV39923.1 .
    RefSeqi WP_012005265.1. NC_009832.1.
    YP_001477051.1. NC_009832.1.

    3D structure databases

    ProteinModelPortali A8G9Y3.
    SMRi A8G9Y3. Positions 1-460.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399741.Spro_0817.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV39923 ; ABV39923 ; Spro_0817 .
    GeneIDi 5603444.
    KEGGi spe:Spro_0817.
    PATRICi 32414278. VBISerPro44537_0846.

    Phylogenomic databases

    eggNOGi COG0007.
    HOGENOMi HOG000290518.
    KOi K02302.
    OMAi LHQQLAW.
    OrthoDBi EOG6DRPFR.

    Enzyme and pathway databases

    UniPathwayi UPA00148 ; UER00211 .
    UPA00148 ; UER00222 .
    UPA00262 ; UER00211 .
    UPA00262 ; UER00222 .
    UPA00262 ; UER00376 .
    BioCyci SPRO399741:GI55-845-MONOMER.

    Family and domain databases

    Gene3Di 1.10.8.210. 1 hit.
    3.30.950.10. 1 hit.
    3.40.1010.10. 1 hit.
    3.40.50.720. 1 hit.
    HAMAPi MF_01646. Siroheme_synth.
    InterProi IPR000878. 4pyrrol_Mease.
    IPR014777. 4pyrrole_Mease_sub1.
    IPR014776. 4pyrrole_Mease_sub2.
    IPR006366. CobA/CysG_C.
    IPR016040. NAD(P)-bd_dom.
    IPR012409. Sirohaem_synth.
    IPR019478. Sirohaem_synthase_dimer_dom.
    IPR006367. Sirohaem_synthase_N.
    IPR003043. Uropor_MeTrfase_CS.
    [Graphical view ]
    Pfami PF10414. CysG_dimeriser. 1 hit.
    PF13241. NAD_binding_7. 1 hit.
    PF00590. TP_methylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
    SUPFAMi SSF53790. SSF53790. 1 hit.
    TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
    TIGR01470. cysG_Nterm. 1 hit.
    PROSITEi PS00839. SUMT_1. 1 hit.
    PS00840. SUMT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 568.

    Entry informationi

    Entry nameiCYSG1_SERP5
    AccessioniPrimary (citable) accession number: A8G9Y3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3