A8G9X8 (CYSH_SERP5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	Spro_0812

Organism

Serratia proteamaculans (strain 568) [Complete proteome] [HAMAP]

Taxonomic identifier

399741 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000057433

Sequences

	Sequence		Length	Mass (Da)
	A8G9X8 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 43137392A5BB93CD	FASTA	244	27,755
	10 20 30 40 50 60 MAEFNLAELN ALPKSGQALS LAVVNGQLET LSAEQRVEWA LEHLPGEFVL SSSFGIQAAV 70 80 90 100 110 120 CLHLVTRIKP DIPVILTDTG YLFPETYQFI DQLADKLKLN LQVFRAEQSP AWQEARYGKL 130 140 150 160 170 180 WEQGVEGIEK YNQINKVEPM NRALETLGGQ TWFAGLRREQ SGSRAHLPVL AVQRGVFKIL 190 200 210 220 230 240 PIIDWDNRKI YQYLTEHGLS YHPLWEQGYL SVGDTHTTQK WEPGMSEEET RFFGLKRECG LHEG

References

[1]

"Complete sequence of chromosome of Serratia proteamaculans 568." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. , Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 568.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000826 Genomic DNA. Translation: ABV39918.1.
RefSeq	YP_001477046.1. NC_009832.1.
3D structure databases
ProteinModelPortal	A8G9X8.
SMR	A8G9X8. Positions 2-244.
ModBase	Search...
Protein-protein interaction databases
STRING	A8G9X8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5603585.
GenomeReviews	Gene locus Spro_0812 in contig CP000826_GR.
KEGG	spe:Spro_0812.
PATRIC	32414270. VBISerPro44537_0842.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
HOGENOM	HBG758022.
OMA	AIHGTRF.
ProtClustDB	PRK02090.
Enzyme and pathway databases
BioCyc	SPRO399741:SPRO_0812-MONOMER.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase_CysH. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. CysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_SERP5

Accession

Primary (citable) accession number: A8G9X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program