Reviewed, UniProtKB/Swiss-Prot A8G9X7 (CYSI_SERP5)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SIR-HP
      Short name=SIRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	Spro_0811

Organism

Serratia proteamaculans (strain 568) [Complete proteome] [HAMAP]

Taxonomic identifier

399741 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Subunit structure	Alpha(8)-beta4. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_1000068767
Sites
	Metal binding	435	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	441	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	480	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	484	1	Iron (siroheme axial ligand) By similarity
	Metal binding	484	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8G9X7-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 805C43AF6C8F21AE	FASTA	571	63,873
	10 20 30 40 50 60 MSDKHPGPLV VEGKLADAER LKKESNFLRG TIAEDLKDGL TGGFTGDNFL LIRFHGMYQQ 70 80 90 100 110 120 DDRDIRAERA EQKLEPRHAM MLRCRLPGGI ISPQQWLGID KFAQESTLYG SIRITNRQTF 130 140 150 160 170 180 QFHGILKGNV KPVHQLLGRL GLDALATAND VNRNVLCTSN PVESELHQEA YEWAKKISEH 190 200 210 220 230 240 LLPRTRAYAE VWLDQEKVAT TDEEPILGAT YLPRKFKTTV VIPPQNDVDL HANDMNFVAI 250 260 270 280 290 300 AKNGKLVGFN LLVGGGLSIE HGNKKTYARQ ASEFGYIPLE HTLAVAEAVV TTQRDWGNRT 310 320 330 340 350 360 DRKNAKTKYT LERVGVDTFR AEVEKRAGIT FEPIRPYEFT GRGDRIGWVK GIDNQWHLTL 370 380 390 400 410 420 FIENGRLLDY PGRPLKTGVA EIARIHKGDF RLTANQNLIV AGVPESEKAK IEALARDHGL 430 440 450 460 470 480 IDDGVSVQRQ NSMACVSFPT CPLAMAEAER FLPEFVTKVE GILHRHGVGD EHIVLRITGC 490 500 510 520 530 540 PNGCGRALLA EIGLVGKAVG RYNLHLGGNR EGTRIPRMYR ENINEEEILR EIDQLVGRWA 550 560 570 TERTAGEGFG DFTIRAGVVR PVVDPAQDFW D

References

[1]

"Complete sequence of chromosome of Serratia proteamaculans 568." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. , Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000826 Genomic DNA. Translation: ABV39917.1.
RefSeq	YP_001477045.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5603584.
GenomeReviews	Gene locus Spro_0811 in contig CP000826_GR.
KEGG	spe:Spro_0811.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8G9X7. ITTTQWQ.
Family and domain databases
HAMAP	MF_01540. [Tree]
InterPro	IPR011786. CysI. IPR006066. Nir_Si_BS. IPR006067. Nir_Sir_4Fe4S. IPR005117. NiRdtase/SiRdtase_haem-b_fer. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_SERP5

Accession

Primary (citable) accession number: A8G9X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)