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A8G9L4

- SYI_SERP5

UniProt

A8G9L4 - SYI_SERP5

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi901 – 9011ZincUniRule annotation
    Metal bindingi904 – 9041ZincUniRule annotation
    Metal bindingi921 – 9211ZincUniRule annotation
    Metal bindingi924 – 9241ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciSPRO399741:GI55-726-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Spro_0698
    OrganismiSerratia proteamaculans (strain 568)
    Taxonomic identifieri399741 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
    ProteomesiUP000007074: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 938938Isoleucine--tRNA ligasePRO_1000070891Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi399741.Spro_0698.

    Structurei

    3D structure databases

    ProteinModelPortaliA8G9L4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8G9L4-1 [UniParc]FASTAAdd to Basket

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    MSDYKNTLNL PETGFPMRGD LAKREPGMLQ RWYEQDLYGM IRTAKKGKKS    50
    FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGMSGFDSP YIPGWDCHGL 100
    PIELKVEQLY GKPGEKLTAA EFRIKCREYA AEQVEGQKKD FIRLGVLGDW 150
    DRPYLTMDFK TEANIIRALG KIISNGHLLK GAKPVHWCTD CRSSLAEAEV 200
    EYYDKTSPSI DVAFHAVDAA AVATKFGVTR FNGPVSLVIW TTTPWTLPAN 250
    RAISLHPEFA YQLVQVEGQC LILAAELVES VMKRAGITHW EVLGSCKGAD 300
    LELMRFEHPF MGFDVPAIMG EHVTLDAGTG AVHTAGGHGP DDFVISQKYG 350
    LEIANPVGPN GCYLTGTHPL LDGKFVFKAN DLIVDLLREK DALLHVEKLV 400
    HSYPCCWRHK TPIIFRATPQ WFISMDQKGL RKQSLEEIKG VQWIPEWGQA 450
    RIETMVANRP DWCISRQRTW GVPMSLFVHK DTEQLHPRSV ELMEEVAKRV 500
    EQDGIQAWWD LDAADILGAD AADYVKVPDT LDVWFDSGST HSSVVDVRPE 550
    FNGHGADMYL EGSDQHRGWF MSSLMISTAM KGKAPYKEVL THGFTVDGQG 600
    RKMSKSIGNT ISPQDVMNKL GGDILRLWVA STDYTGEIAV SDEILKRSAD 650
    SYRRIRNTAR FLLANLNGFE PSTDCVAPEE MVVLDRWAVG RALAAQQDIE 700
    KAYANYDFHE VVQRLMQFCS VEMGSFYLDI IKDRQYTAKS DSVARRSCQT 750
    ALFHIAEALV RWMAPIMSFT ADEIWAFLPG KRAQYVFTEE WYDGLFGLAE 800
    GEGMNDSFWA ELLKVRGEVN KVLEQARADK RLGGSLEAAV TLYADSELAE 850
    RLNSLQDELR FVLLTSGARV APLADAPADA QAAELVKGLK IAFSTAEGEK 900
    CPRCWHYTTD IGLVAEHADL CGRCVVNVAG DGEKRKFA 938
    Length:938
    Mass (Da):104,735
    Last modified:November 13, 2007 - v1
    Checksum:iFEEBCE54CDD2D4BD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000826 Genomic DNA. Translation: ABV39804.1.
    RefSeqiYP_001476932.1. NC_009832.1.

    Genome annotation databases

    EnsemblBacteriaiABV39804; ABV39804; Spro_0698.
    GeneIDi5605478.
    KEGGispe:Spro_0698.
    PATRICi32414034. VBISerPro44537_0724.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000826 Genomic DNA. Translation: ABV39804.1 .
    RefSeqi YP_001476932.1. NC_009832.1.

    3D structure databases

    ProteinModelPortali A8G9L4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 399741.Spro_0698.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV39804 ; ABV39804 ; Spro_0698 .
    GeneIDi 5605478.
    KEGGi spe:Spro_0698.
    PATRICi 32414034. VBISerPro44537_0724.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci SPRO399741:GI55-726-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 568.

    Entry informationi

    Entry nameiSYI_SERP5
    AccessioniPrimary (citable) accession number: A8G9L4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3