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A8G9L4

- SYI_SERP5

UniProt

A8G9L4 - SYI_SERP5

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Spro_0698
Organism
Serratia proteamaculans (strain 568)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylate By similarity
Binding sitei605 – 6051ATP By similarity
Metal bindingi901 – 9011Zinc By similarity
Metal bindingi904 – 9041Zinc By similarity
Metal bindingi921 – 9211Zinc By similarity
Metal bindingi924 – 9241Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSPRO399741:GI55-726-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Spro_0698
OrganismiSerratia proteamaculans (strain 568)
Taxonomic identifieri399741 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia
ProteomesiUP000007074: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 938938Isoleucine--tRNA ligaseUniRule annotationPRO_1000070891Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi399741.Spro_0698.

Structurei

3D structure databases

ProteinModelPortaliA8G9L4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi602 – 6065"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8G9L4-1 [UniParc]FASTAAdd to Basket

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MSDYKNTLNL PETGFPMRGD LAKREPGMLQ RWYEQDLYGM IRTAKKGKKS    50
FILHDGPPYA NGSIHIGHSV NKILKDIIIK SKGMSGFDSP YIPGWDCHGL 100
PIELKVEQLY GKPGEKLTAA EFRIKCREYA AEQVEGQKKD FIRLGVLGDW 150
DRPYLTMDFK TEANIIRALG KIISNGHLLK GAKPVHWCTD CRSSLAEAEV 200
EYYDKTSPSI DVAFHAVDAA AVATKFGVTR FNGPVSLVIW TTTPWTLPAN 250
RAISLHPEFA YQLVQVEGQC LILAAELVES VMKRAGITHW EVLGSCKGAD 300
LELMRFEHPF MGFDVPAIMG EHVTLDAGTG AVHTAGGHGP DDFVISQKYG 350
LEIANPVGPN GCYLTGTHPL LDGKFVFKAN DLIVDLLREK DALLHVEKLV 400
HSYPCCWRHK TPIIFRATPQ WFISMDQKGL RKQSLEEIKG VQWIPEWGQA 450
RIETMVANRP DWCISRQRTW GVPMSLFVHK DTEQLHPRSV ELMEEVAKRV 500
EQDGIQAWWD LDAADILGAD AADYVKVPDT LDVWFDSGST HSSVVDVRPE 550
FNGHGADMYL EGSDQHRGWF MSSLMISTAM KGKAPYKEVL THGFTVDGQG 600
RKMSKSIGNT ISPQDVMNKL GGDILRLWVA STDYTGEIAV SDEILKRSAD 650
SYRRIRNTAR FLLANLNGFE PSTDCVAPEE MVVLDRWAVG RALAAQQDIE 700
KAYANYDFHE VVQRLMQFCS VEMGSFYLDI IKDRQYTAKS DSVARRSCQT 750
ALFHIAEALV RWMAPIMSFT ADEIWAFLPG KRAQYVFTEE WYDGLFGLAE 800
GEGMNDSFWA ELLKVRGEVN KVLEQARADK RLGGSLEAAV TLYADSELAE 850
RLNSLQDELR FVLLTSGARV APLADAPADA QAAELVKGLK IAFSTAEGEK 900
CPRCWHYTTD IGLVAEHADL CGRCVVNVAG DGEKRKFA 938
Length:938
Mass (Da):104,735
Last modified:November 13, 2007 - v1
Checksum:iFEEBCE54CDD2D4BD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000826 Genomic DNA. Translation: ABV39804.1.
RefSeqiYP_001476932.1. NC_009832.1.

Genome annotation databases

EnsemblBacteriaiABV39804; ABV39804; Spro_0698.
GeneIDi5605478.
KEGGispe:Spro_0698.
PATRICi32414034. VBISerPro44537_0724.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000826 Genomic DNA. Translation: ABV39804.1 .
RefSeqi YP_001476932.1. NC_009832.1.

3D structure databases

ProteinModelPortali A8G9L4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 399741.Spro_0698.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV39804 ; ABV39804 ; Spro_0698 .
GeneIDi 5605478.
KEGGi spe:Spro_0698.
PATRICi 32414034. VBISerPro44537_0724.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci SPRO399741:GI55-726-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 568.

Entry informationi

Entry nameiSYI_SERP5
AccessioniPrimary (citable) accession number: A8G9L4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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