A8G7S7 (GHRB_SERP5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyoxylate/hydroxypyruvate reductase B
EC=1.1.1.79
EC=1.1.1.81

Gene names

Name:	ghrB
Ordered Locus Names:	Spro_0057

Organism

Serratia proteamaculans (strain 568) [Complete proteome] [HAMAP]

Taxonomic identifier

399741 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01667
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667
Subunit structure	Homodimer By similarity. HAMAP MF_01667
Subcellular location	Cytoplasm By similarity HAMAP MF_01667.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: EC hydroxypyruvate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667		PRO_0000348398
Sites
	Active site	237	1	By similarity
	Active site	266	1	By similarity
	Active site	285	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8G7S7 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 65AC6FF0BC3502DC	FASTA	325	35,463
	10 20 30 40 50 60 MKPSIVLYKN IPADLRERLE QHFTVHAFDG LTPDNHDELR QALQQAEGII GSGGKIDEAF 70 80 90 100 110 120 LQQTPKLRAA STISVGYDNF DVDALNAHNV LLMHTPTVLT ETVADTIMSL VLATARRVVE 130 140 150 160 170 180 VAERVKAGEW QGSIGADWFG VDVHHKTIGI LGMGRIGLAL AQRAHFGFGM PVLYNARRTH 190 200 210 220 230 240 EEAEQRFNAR RCDLDTLLAE SDFICITLPL TDETFHMISR DQLAKMKKSG ILINAGRGPV 250 260 270 280 290 300 VDEAALIEAL QNGTIHAAGL DVFEKEPLPV SSPLLTLPNV VALPHIGSAT HETRYGMAEC 310 320 AVDNLIAALT GTVKENCVNP QLLKK

References

[1]

"Complete sequence of chromosome of Serratia proteamaculans 568." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E. , Taghavi S., Newman L., Vangronsveld J., van der Lelie D., Richardson P. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 568.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000826 Genomic DNA. Translation: ABV39167.1.
RefSeq	YP_001476295.1. NC_009832.1.
3D structure databases
ProteinModelPortal	A8G7S7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8G7S7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5604824.
GenomeReviews	Gene locus Spro_0057 in contig CP000826_GR.
KEGG	spe:Spro_0057.
PATRIC	32412722. VBISerPro44537_0098.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1052.
HOGENOM	HBG731446.
OMA	HALTEKK.
ProtClustDB	PRK15409.
Enzyme and pathway databases
BioCyc	SPRO399741:SPRO_0057-MONOMER.
Family and domain databases
HAMAP	MF_01667. 2-Hacid_dh_C_GhrB. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR023756. Glyo/OHPyrv_Rdtase_B. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K00090.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRB_SERP5

Accession

Primary (citable) accession number: A8G7S7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program