A8G6W1 (QUEF_PROM2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	P9215_17291

Organism

Prochlorococcus marinus (strain MIT 9215) [Complete proteome] [HAMAP]

Taxonomic identifier

93060 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	136 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00818
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00818
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00818
Subcellular location	Cytoplasm Probable HAMAP MF_00818.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 136	136	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00818		PRO_1000062402

Sequences

	Sequence		Length	Mass (Da)
	A8G6W1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 6A5AF960B851C380	FASTA	136	15,827
	10 20 30 40 50 60 MSTAKLDDST QRPLYGERII KESKIICFEN PNKKRIYEIS IDLPEFTCKC PFSGYPDFAK 70 80 90 100 110 120 LNIIYQPNLR VYELKSLKLY INNFRDIKIS HEEVVNRIMD DLLSAGSPHW IHLNASFNPR 130 GNVSMQLDIF SGQKKN

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9215.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000825 Genomic DNA. Translation: ABV51342.1.
RefSeq	YP_001484928.1. NC_009840.1.
3D structure databases
ProteinModelPortal	A8G6W1.
ModBase	Search...
Protein-protein interaction databases
STRING	A8G6W1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5614942.
GenomeReviews	Gene locus P9215_17291 in contig CP000825_GR.
KEGG	pmh:P9215_17291.
PATRIC	22993056. VBIProMar119824_1788.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0780.
HOGENOM	HBG294103.
OMA	FRNAGIF.
PhylomeDB	A8G6W1.
ProtClustDB	PRK13258.
Enzyme and pathway databases
BioCyc	PMAR93060:P9215_17291-MONOMER.
Family and domain databases
HAMAP	MF_00818. QueF_type1. [Tree]
InterPro	IPR016856. CN_OxRdtase_NADPH-dep_QueF. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K09457.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF027377. Nitrile_oxidored_QueF. 1 hit.
TIGRFAMs	TIGR03139. QueF-II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_PROM2

Accession

Primary (citable) accession number: A8G6W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program