Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A8G5G3

- BIOB_PROM2

UniProt

A8G5G3 - BIOB_PROM2

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Biotin synthase

Gene

bioB

Organism
Prochlorococcus marinus (strain MIT 9215)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi105 – 1051Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi137 – 1371Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi197 – 1971Iron-sulfur 2 (2Fe-2S)UniRule annotation
Metal bindingi269 – 2691Iron-sulfur 2 (2Fe-2S)UniRule annotation

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciPMAR93060:GI08-1262-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:P9215_12291
OrganismiProchlorococcus marinus (strain MIT 9215)
Taxonomic identifieri93060 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002014: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 335335Biotin synthasePRO_0000381538Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi93060.P9215_12291.

Structurei

3D structure databases

ProteinModelPortaliA8G5G3.
SMRiA8G5G3. Positions 13-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiIDLMWEA.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A8G5G3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MANSNNQLLK EIRFDWNKEE ILEILNMPLI DLMWESQTIH RKFNKYDIQL
60 70 80 90 100
ASLFSVKTGG CEENCSYCSQ SIYSASEIKS HPQFQVEEVL ARAQIAKKEG
110 120 130 140 150
ADRFCMGWAW REIRDGKSFN AMLEMVSGVR DLGMEACVTA GMLTEEQAAR
160 170 180 190 200
LADAGLTAYN HNLDTSPEHY KNIITTRTYQ DRLDTIKRVR NAGINVCCGG
210 220 230 240 250
IIGLGETNGD RASLLKVLSN MNPHPESVPI NSLVAIEGTG LEDNQEIDSI
260 270 280 290 300
EMIRMIATAR ILMPKSKIRL SAGREKLSKE AQILCFQCGA NSIFYGDELL
310 320 330
TTSNPSFQSD RKLLKEVGVS FNKDFETREK TLSSL
Length:335
Mass (Da):37,581
Last modified:November 13, 2007 - v1
Checksum:i6B7FB559E82B4D97
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000825 Genomic DNA. Translation: ABV50844.1.
RefSeqiYP_001484430.1. NC_009840.1.

Genome annotation databases

EnsemblBacteriaiABV50844; ABV50844; P9215_12291.
GeneIDi5615150.
KEGGipmh:P9215_12291.
PATRICi22991960. VBIProMar119824_1250.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000825 Genomic DNA. Translation: ABV50844.1 .
RefSeqi YP_001484430.1. NC_009840.1.

3D structure databases

ProteinModelPortali A8G5G3.
SMRi A8G5G3. Positions 13-320.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 93060.P9215_12291.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABV50844 ; ABV50844 ; P9215_12291 .
GeneIDi 5615150.
KEGGi pmh:P9215_12291.
PATRICi 22991960. VBIProMar119824_1250.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi IDLMWEA.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci PMAR93060:GI08-1262-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9215.

Entry informationi

Entry nameiBIOB_PROM2
AccessioniPrimary (citable) accession number: A8G5G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 13, 2007
Last modified: October 29, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3