A8G4Z1 (ACSF_PROM2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
EC=1.14.13.81

Gene names

Name:	acsF
Ordered Locus Names:	P9215_10571

Organism

Prochlorococcus marinus (strain MIT 9215) [Complete proteome] [HAMAP]

Taxonomic identifier

93060 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity. HAMAP MF_01840
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity. HAMAP MF_01840
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent). HAMAP MF_01840
Sequence similarities	Belongs to the AcsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840		PRO_1000070546

Sequences

	Sequence		Length	Mass (Da)
	A8G4Z1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: B0D8BEF05AE66A43	FASTA	390	45,941
	10 20 30 40 50 60 MSKSTIEPKN KKSVNRGKEI AKDTILTPNF YTTDFEAMEK MDLSINEDEL EAICEEFRKD 70 80 90 100 110 120 YNRHHFVRNK EFEGAADKLD PETRELFVDF LEGSCTSEFS GFLLYKELSK RIKDKNPLLA 130 140 150 160 170 180 ECFAHMARDE ARHAGFLNKS MNDFGLQLDL GFLTANKDYT YFPPRSIFYA TYISEKIGYW 190 200 210 220 230 240 RYIAIFRHLE KNPEGKIFPL FNFFENWCQD ENRHGDFFDA LMKAQPRSVK SLSNKITIGG 250 260 270 280 290 300 STFTHPLFDY FHRFRYFLNN HPFVSKLWSR FFLLAVFATM YIRDQGTKKD FYSALGLDAR 310 320 330 340 350 360 EYDQFVINKT NETSARVFPV VLNVFDDSFY RRLDSIVENG KRLSEIDKKD NQNLNKVLSK 370 380 390 IPIFISNGYQ LLRLYLLKPL DSKDYQPSIR

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9215.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000825 Genomic DNA. Translation: ABV50672.1.
RefSeq	YP_001484258.1. NC_009840.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8G4Z1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5615291.
GenomeReviews	Gene locus P9215_10571 in contig CP000825_GR.
KEGG	pmh:P9215_10571.
PATRIC	22991608. VBIProMar119824_1075.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG10633.
HOGENOM	HBG284843.
OMA	HGDFFDA.
PhylomeDB	A8G4Z1.
ProtClustDB	PRK13654.
Enzyme and pathway databases
BioCyc	PMAR93060:P9215_10571-MONOMER.
Family and domain databases
HAMAP	MF_01840. AcsF. [Tree]
InterPro	IPR008434. AcsF. IPR012347. Ferritin-rel. IPR009078. Ferritin/RR-like. IPR003251. Rubrerythrin. [Graphical view]
Gene3D	G3DSA:1.20.1260.10. Ferritin_rel. 1 hit.
KO	K04035.
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_PROM2

Accession

Primary (citable) accession number: A8G4Z1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program