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A8G4T7 (SYL_PROM2) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:P9215_10031
OrganismProchlorococcus marinus (strain MIT 9215) [Complete proteome] [HAMAP]
Taxonomic identifier93060 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length856 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 856856Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000057346

Regions

Motif53 – 6311"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8G4T7 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: CB977FD2125F5D68

FASTA85698,952
        10         20         30         40         50         60 
MISPDKQYES FTNLYNPSEI EKKWQLIWTE NNLYKTDELT ENSDKFYALS MFPYPSGNLH 

        70         80         90        100        110        120 
MGHVRNYVIT DLIARFHRFK GKSVLHPMGW DAFGLPAENA AIERGISPSV WTKQNISHMR 

       130        140        150        160        170        180 
SQLKLLGLSV DWDREFATCD ENYYIWTQYL FLELYKAGLV YQKESEVNWD PVDNTVLANE 

       190        200        210        220        230        240 
QVDSEGKSWR SGAVVEKKLL KQWFLRITNY ADELLKDLEK LDNWPERVKI MQDNWIGKSI 

       250        260        270        280        290        300 
GTNINFNLNT NPEEKITVFT TRPDTLFGVT YLAISVNHSL IKYISDQETI QDIENLKQYL 

       310        320        330        340        350        360 
KNNKNNELEK IGIKTSLIAI NPINSEPIPI WVASYVLDEY GTGAVMGVPA HDQRDFEFAK 

       370        380        390        400        410        420 
KNNIDIKQVI INDKSEKTNE LDKAYVENGY LMNSDQYNFM ENTIAKLKIS EEGVDNGWAE 

       430        440        450        460        470        480 
NKIQYRLRDW LISRQRYWGC PIPIVNCKKC GSVPLKQSEL PVALPKDIDI SANKINALGD 

       490        500        510        520        530        540 
NNHWINTTCP KCGIAAKKET DTMDTFMCSS WYFLRYPSSK CSNKPFEKIE INKWLPVDQY 

       550        560        570        580        590        600 
VGGVEHAILH LLYARFFTKA LRDNELFEID EPFKKLLTQG MVQAAAYKNN KTGKYVSPSD 

       610        620        630        640        650        660 
INDLSNPTDP IDNTKLEVLF EKMSKSKYNG IDPESVIKKY GADTARMFIL FKAPPEKDLE 

       670        680        690        700        710        720 
WGDTDVEGQF RFLNRIWKLY INCAKDINSK SNSYPDREKS LIKSMNIAIK EISNDILNNQ 

       730        740        750        760        770        780 
FNTAISELMK FYNSLANSIN DVNNTLKIEA LKTFCILLAP FAPHIAEEIW HLIGFKKSVH 

       790        800        810        820        830        840 
LEYWPSFNAE ALKEDSYELV IQVNGKVRDK VKINNDMSED QIKELTLKRP NILKWTQDKE 

       850 
IRKIIIVKGK IINIVV 

« Hide

References

[1]"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus."
Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W.
PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MIT 9215.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000825 Genomic DNA. Translation: ABV50618.1.
RefSeqYP_001484204.1. NC_009840.1.

3D structure databases

ProteinModelPortalA8G4T7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING93060.P9215_10031.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV50618; ABV50618; P9215_10031.
GeneID5615845.
KEGGpmh:P9215_10031.
PATRIC22991502. VBIProMar119824_1023.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycPMAR93060:GI08-1034-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PROM2
AccessionPrimary (citable) accession number: A8G4T7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries