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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Prochlorococcus marinus (strain MIT 9215)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei625 – 6251ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMAR93060:GI08-1034-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:P9215_10031
OrganismiProchlorococcus marinus (strain MIT 9215)
Taxonomic identifieri93060 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000002014 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 856856Leucine--tRNA ligasePRO_1000057346Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi93060.P9215_10031.

Structurei

3D structure databases

ProteinModelPortaliA8G4T7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi53 – 6311"HIGH" regionAdd
BLAST
Motifi622 – 6265"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8G4T7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISPDKQYES FTNLYNPSEI EKKWQLIWTE NNLYKTDELT ENSDKFYALS
60 70 80 90 100
MFPYPSGNLH MGHVRNYVIT DLIARFHRFK GKSVLHPMGW DAFGLPAENA
110 120 130 140 150
AIERGISPSV WTKQNISHMR SQLKLLGLSV DWDREFATCD ENYYIWTQYL
160 170 180 190 200
FLELYKAGLV YQKESEVNWD PVDNTVLANE QVDSEGKSWR SGAVVEKKLL
210 220 230 240 250
KQWFLRITNY ADELLKDLEK LDNWPERVKI MQDNWIGKSI GTNINFNLNT
260 270 280 290 300
NPEEKITVFT TRPDTLFGVT YLAISVNHSL IKYISDQETI QDIENLKQYL
310 320 330 340 350
KNNKNNELEK IGIKTSLIAI NPINSEPIPI WVASYVLDEY GTGAVMGVPA
360 370 380 390 400
HDQRDFEFAK KNNIDIKQVI INDKSEKTNE LDKAYVENGY LMNSDQYNFM
410 420 430 440 450
ENTIAKLKIS EEGVDNGWAE NKIQYRLRDW LISRQRYWGC PIPIVNCKKC
460 470 480 490 500
GSVPLKQSEL PVALPKDIDI SANKINALGD NNHWINTTCP KCGIAAKKET
510 520 530 540 550
DTMDTFMCSS WYFLRYPSSK CSNKPFEKIE INKWLPVDQY VGGVEHAILH
560 570 580 590 600
LLYARFFTKA LRDNELFEID EPFKKLLTQG MVQAAAYKNN KTGKYVSPSD
610 620 630 640 650
INDLSNPTDP IDNTKLEVLF EKMSKSKYNG IDPESVIKKY GADTARMFIL
660 670 680 690 700
FKAPPEKDLE WGDTDVEGQF RFLNRIWKLY INCAKDINSK SNSYPDREKS
710 720 730 740 750
LIKSMNIAIK EISNDILNNQ FNTAISELMK FYNSLANSIN DVNNTLKIEA
760 770 780 790 800
LKTFCILLAP FAPHIAEEIW HLIGFKKSVH LEYWPSFNAE ALKEDSYELV
810 820 830 840 850
IQVNGKVRDK VKINNDMSED QIKELTLKRP NILKWTQDKE IRKIIIVKGK

IINIVV
Length:856
Mass (Da):98,952
Last modified:November 12, 2007 - v1
Checksum:iCB977FD2125F5D68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000825 Genomic DNA. Translation: ABV50618.1.
RefSeqiWP_012007705.1. NC_009840.1.
YP_001484204.1. NC_009840.1.

Genome annotation databases

EnsemblBacteriaiABV50618; ABV50618; P9215_10031.
KEGGipmh:P9215_10031.
PATRICi22991502. VBIProMar119824_1023.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000825 Genomic DNA. Translation: ABV50618.1.
RefSeqiWP_012007705.1. NC_009840.1.
YP_001484204.1. NC_009840.1.

3D structure databases

ProteinModelPortaliA8G4T7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93060.P9215_10031.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABV50618; ABV50618; P9215_10031.
KEGGipmh:P9215_10031.
PATRICi22991502. VBIProMar119824_1023.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciPMAR93060:GI08-1034-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9215.

Entry informationi

Entry nameiSYL_PROM2
AccessioniPrimary (citable) accession number: A8G4T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 4, 2008
Last sequence update: November 12, 2007
Last modified: March 31, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.