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A8G4P2 (ISPE_PROM2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Short name=CMK
EC=2.7.1.148
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase
Gene names
Name:ispE
Ordered Locus Names:P9215_09581
OrganismProchlorococcus marinus (strain MIT 9215) [Complete proteome] [HAMAP]
Taxonomic identifier93060 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061

Catalytic activity

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061

Pathway

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061

Sequence similarities

Belongs to the GHMP kinase family. IspE subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3113114-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061
PRO_1000057424

Regions

Nucleotide binding100 – 11011ATP Potential

Sites

Active site161 By similarity
Active site1421 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8G4P2 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: C7A64D7DB4483A35

FASTA31134,714
        10         20         30         40         50         60 
MQDLAGKKII IKSPAKINLH LEVIGKREDG FHELAMIMQN IDLFDYLEFQ INNEGLIKLE 

        70         80         90        100        110        120 
SDCTDLSLSS DNLIVKSANL LRKKLNIDCG ANIFLRKNIP IGAGLAGGSS NAAATLIGLN 

       130        140        150        160        170        180 
KLWDLNVDQK TLFSLASTLG SDIPFFINGG IQLCFGRGEI LEKLDSNFDY GVILLKNPNV 

       190        200        210        220        230        240 
SVSTAETYSK YSNRFCYQYL TNGEMIENIR NNLRDNGLNN LNFDKQHLTI KNDLQLVVEN 

       250        260        270        280        290        300 
DNDSVKEALY LLSKLENCLT FSMSGSGPTC FAIFKDIETA KKELNANSKL FEDKGYDAWV 

       310 
CTFFEKGITF I 

« Hide

References

[1]"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus."
Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W.
PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MIT 9215.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000825 Genomic DNA. Translation: ABV50573.1.
RefSeqYP_001484159.1. NC_009840.1.

3D structure databases

ProteinModelPortalA8G4P2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING93060.P9215_09581.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV50573; ABV50573; P9215_09581.
GeneID5616081.
KEGGpmh:P9215_09581.
PATRIC22991412. VBIProMar119824_0979.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1947.
HOGENOMHOG000019600.
KOK00919.
OMAELAMIMQ.
OrthoDBEOG62VNQ2.

Enzyme and pathway databases

BioCycPMAR93060:GI08-987-MONOMER.
UniPathwayUPA00056; UER00094.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.70.890. 1 hit.
HAMAPMF_00061. IspE.
InterProIPR013750. GHMP_kinase_C_dom.
IPR006204. GHMP_kinase_N_dom.
IPR004424. IspE.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR20861:SF2. PTHR20861:SF2. 1 hit.
PfamPF08544. GHMP_kinases_C. 1 hit.
PF00288. GHMP_kinases_N. 1 hit.
[Graphical view]
PIRSFPIRSF010376. IspE. 1 hit.
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55060. SSF55060. 1 hit.
TIGRFAMsTIGR00154. ispE. 1 hit.
ProtoNetSearch...

Entry information

Entry nameISPE_PROM2
AccessionPrimary (citable) accession number: A8G4P2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: November 13, 2007
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways