A8G3V6 (PROA_PROM2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	P9215_06721

Organism

Prochlorococcus marinus (strain MIT 9215) [Complete proteome] [HAMAP]

Taxonomic identifier

93060 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Prochlorales › Prochlorococcaceae › Prochlorococcus › Prochlorococcus marinus

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP-Rule MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP-Rule MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP-Rule MF_00412
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Gamma-glutamyl phosphate reductase HAMAP-Rule MF_00412		PRO_1000060843

Sequences

	Sequence		Length	Mass (Da)
	A8G3V6 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 29680531FC3AAFBE	FASTA	436	47,862
	10 20 30 40 50 60 MANIFEVPQP DNDLLEKAEK VRLASIKISQ TENKNRIKAL NFMADYLEKN SKEILEANNA 70 80 90 100 110 120 DYSSAEKKGI SKALLSRLKL SRAKLNAGIE GVRKVGDLAD PVNQVQIKKE LSKGLILERK 130 140 150 160 170 180 TVPIGVLGII FESRPDAVMQ ISSLAIRSGN GVMLKGGSEA NLTNTSIVKA LQEGLNESGL 190 200 210 220 230 240 DRNAICLLTS RKDSMAMLNL EKYINLIIPR GSNELVKFIQ ENTRIPVLGH ADGICHLFID 250 260 270 280 290 300 IEANLEMALS VALDSKIQYP AACNAIETLL VHKDIAPAFL EKAIPLFNSN DVKLIGDNRS 310 320 330 340 350 360 VELGLKYEAS LQDWKTEYLD LILSIKIVNN VEEAITHIQK YSSKHTDGII TENSNTANKF 370 380 390 400 410 420 MNVVDSSGVF HNCSTRFADG FRYGFGAEVG ISTQTLPPRG PVGLEGLVTY KYFLKGGGHI 430 VDDFSSGKAI YTHKDL

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9215.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000825 Genomic DNA. Translation: ABV50287.1.
RefSeq	YP_001483873.1. NC_009840.1.
3D structure databases
ProteinModelPortal	A8G3V6.
ModBase	Search...
Protein-protein interaction databases
STRING	93060.P9215_06721.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABV50287; ABV50287; P9215_06721.
GeneID	5614732.
KEGG	pmh:P9215_06721.
PATRIC	22990808. VBIProMar119824_0679.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HOG000246356.
KO	K00147.
OMA	EVGISTQ.
ProtClustDB	PRK00197.
Enzyme and pathway databases
BioCyc	PMAR93060:GI08-719-MONOMER.
UniPathway	UPA00098; UER00360.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 2 hits.
HAMAP	MF_00412. ProA.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_PROM2

Accession

Primary (citable) accession number: A8G3V6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 13, 2007
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program