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A8G2P6

- SYI_PROM2

UniProt

A8G2P6 - SYI_PROM2

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain MIT 9215)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 52 (01 Oct 2014)
      Sequence version 1 (13 Nov 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei582 – 5821Aminoacyl-adenylateUniRule annotation
    Binding sitei626 – 6261ATPUniRule annotation
    Metal bindingi936 – 9361ZincUniRule annotation
    Metal bindingi939 – 9391ZincUniRule annotation
    Metal bindingi956 – 9561ZincUniRule annotation
    Metal bindingi959 – 9591ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPMAR93060:GI08-265-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:P9215_02601
    OrganismiProchlorococcus marinus (strain MIT 9215)
    Taxonomic identifieri93060 [NCBI]
    Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
    ProteomesiUP000002014: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 965965Isoleucine--tRNA ligasePRO_1000070890Add
    BLAST

    Proteomic databases

    PRIDEiA8G2P6.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi93060.P9215_02601.

    Structurei

    3D structure databases

    ProteinModelPortaliA8G2P6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi68 – 7811"HIGH" regionAdd
    BLAST
    Motifi623 – 6275"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiEVMDEIT.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8G2P6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSQNSKEQK SEFSYKETLN LLKTDFSMRA NSVLREPEIQ DFWAKNNIDF    50
    ELGLNNSGKT FTLHDGPPYA NGSLHMGHAL NKVLKDIINK YKTLKGFKVH 100
    YVPGWDCHGL PIELKVLQNL KSHERKNLNS LSLRKKATDY AHKQINNQME 150
    GFKRWGIWGD WDNPYLTLKK SYESAQIGVF GKMFLNGYIY RGLKPVHWSP 200
    SSRTALAEAE LEYPDDHYSK SIYVSLKITK VPDKVLLKGC QENPNIKKES 250
    FLGNSFITIW TTTPWTIPAN EAVAVNPKIS YVFVIDEDKR IFLFAKNLFS 300
    EISEKFNKIF KILFEVKGSS LENIEYQHPT KNKNCRIVIG GDYITTESGT 350
    GIVHTAPGHG IDDFNVGQKY DLPITCVVDE KGNLNEYSGQ FKGSNVLKDS 400
    NDLIIEYLKG KNLLLLQENY KHRYPYDWRT KKPTIFRATE QWFASVDGFR 450
    SSALKAIEDV EWMPATGKKR IYSMVVGRGD WCISRQRSWG VPIPVFYKKN 500
    GNEILLNSEI INHIQELFSE YGADIWWDWE VKNLLPENYL KESDLWEKGT 550
    DTMDVWFDSG SSWAAVCEQR SELKYPADLY LEGSDQHRGW FQSSLLTSVA 600
    VNNKPPYKRV LTHGFALDEN GRKMSKSLGN VVDPNIIING GNNKKSEPAY 650
    GADVLRLWVS SVDYSVDVPI GSNILKQLSD VYRKVRNTAR YLLGNIHDYD 700
    PNIDSFEIDQ LPLLDQWMLG RLVEVSSQIS HAYESYEFSR FFQILQSFCV 750
    VDLSNFYLDI AKDRLYVSAK SQFRRRSCQF VMSKVVENLA VLISPVLCHM 800
    AEDIWQNIPY LTKEKSVFQR GWPIFSQSWK NESLNQHITN LRNLRVEINK 850
    AIEGCRNKQI IGAALETEVN YLPKDKFVKD SLNWLRKFGN EDVDLFRDWL 900
    IVSNFQVVSY LAENSLIIDS NELGEIQILK ARGLKCDRCW HYQKEIVSGI 950
    QNTKLCKRCA KIINL 965
    Length:965
    Mass (Da):111,282
    Last modified:November 13, 2007 - v1
    Checksum:i0AC02A57042FD2E0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000825 Genomic DNA. Translation: ABV49877.1.
    RefSeqiYP_001483463.1. NC_009840.1.

    Genome annotation databases

    EnsemblBacteriaiABV49877; ABV49877; P9215_02601.
    GeneIDi5616291.
    KEGGipmh:P9215_02601.
    PATRICi22989932. VBIProMar119824_0257.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000825 Genomic DNA. Translation: ABV49877.1 .
    RefSeqi YP_001483463.1. NC_009840.1.

    3D structure databases

    ProteinModelPortali A8G2P6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 93060.P9215_02601.

    Proteomic databases

    PRIDEi A8G2P6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABV49877 ; ABV49877 ; P9215_02601 .
    GeneIDi 5616291.
    KEGGi pmh:P9215_02601.
    PATRICi 22989932. VBIProMar119824_0257.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi EVMDEIT.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PMAR93060:GI08-265-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MIT 9215.

    Entry informationi

    Entry nameiSYI_PROM2
    AccessioniPrimary (citable) accession number: A8G2P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: November 13, 2007
    Last modified: October 1, 2014
    This is version 52 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3