A8G1Q8 (RLMF_SHESH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	Ssed_4429

Organism

Shewanella sediminis (strain HAW-EB3) [Complete proteome] [HAMAP]

Taxonomic identifier

425104 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	404 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 404	404	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349961

Sequences

	Sequence		Length	Mass (Da)
	A8G1Q8 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: BF7474AFFB54E1C9	FASTA	404	45,236
	10 20 30 40 50 60 MTKHSQKQNR QKQTTQKQTR RKKPAGKLKA KSEAKLDTRG KPETTEKKGL HERNLHRDGY 70 80 90 100 110 120 DFEQLIEASP ALKPYVRPNP YGNLSIDFAD PLAVKALNLA LLQLHYRIEY WDIPAGFLCP 130 140 150 160 170 180 PIPGRVDYLH YIADLLAGID SDTVGDKAEE QVEEIGTRQN VPYASKPESS APKQRYKSQK 190 200 210 220 230 240 RMKINALDIG TGANGIYPIL GIQAYGWRFV ASDVDPLSIE NVNRIVGQNK ALQGKLKTRL 250 260 270 280 290 300 QTDHQKVFHG IIQADDRFDI TLCNPPFHAS LSEASEGSLR KVKNLAANRA AKGHKPEPAA 310 320 330 340 350 360 SKAKPDANEL NFGGQKAELW CEGGEKQFLA NMIRESKDFA TQCLWFTSLV SKKENLQPCY 370 380 390 400 AALEKVGAVT VKTIDMAQGN KLTRVLAWSY LTPKQQALWA KYRS

References

[1]

"Complete sequence of Shewanella sediminis HAW-EB3." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAW-EB3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000821 Genomic DNA. Translation: ABV39031.1.
RefSeq	YP_001476159.1. NC_009831.1.
3D structure databases
ProteinModelPortal	A8G1Q8.
ModBase	Search...
Protein-protein interaction databases
STRING	A8G1Q8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5611665.
GenomeReviews	Gene locus Ssed_4429 in contig CP000821_GR.
KEGG	sse:Ssed_4429.
NMPDR	fig|425104.3.peg.3967.
PATRIC	23566848. VBISheSed62411_4707.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	WSFVGSD.
ProtClustDB	PRK11727.
Enzyme and pathway databases
BioCyc	SSED425104:SSED_4429-MONOMER.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 2 hits. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_SHESH

Accession

Primary (citable) accession number: A8G1Q8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program