A8G0Z3 (ILVD_SHESH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Ssed_4162

Organism

Shewanella sediminis (strain HAW-EB3) [Complete proteome] [HAMAP]

Taxonomic identifier

425104 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000073991
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A8G0Z3 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 85EDF5A504E7DFBF	FASTA	616	65,285
	10 20 30 40 50 60 MPKLRSATST EGRNMAGARA LWRATGVKEN DFGKPIIAIA NSFTQFVPGH VHLKDMGSLV 70 80 90 100 110 120 AGAIEEAGGI AKEFNTIAVD DGIAMGHGGM LYSLPSRELI SDSVEYMVNA HCADALVCIS 130 140 150 160 170 180 NCDKITPGMM MAALRLNIPV IFVSGGPMEA GKTKLSDKII KLDLVDAMVA GADDRVSDED 190 200 210 220 230 240 SEQIERSACP TCGSCSGMFT ANSMNCLTEA LGLSLPGNGS MLATHADRRE LFLEAGRRIM 250 260 270 280 290 300 DLTTRYYKHD DESALPRNIA NFNAFENAMT LDIAMGGSSN TVLHLLAAAQ EGEVDFTMED 310 320 330 340 350 360 IDRLSRLVPH LCKVAPSTPE YHMEDVHRAG GVMGILGELD RAGLIHNDAY HVAGASLKEV 370 380 390 400 410 420 LAKWDIAQSS DEAVHRFFSA GPAGIPTTKA FSQACRWDSV DNDRVGGCIR KREFAFSQEG 430 440 450 460 470 480 GLAVLSGNIA VDGCIVKTAG VDEDNHTFIG SARVYESQDD AVAGILGGEV VAGDVVVIRY 490 500 510 520 530 540 EGPKGGPGMQ EMLYPTSYLK SRGLGKACAL ITDGRFSGGT SGLSIGHVSP EAAAGGTIGL 550 560 570 580 590 600 VETGDRIEID IPARSIKLAI SDIELAARRS AMEARGKQAW KPVGRVREVS LALKAYALLA 610 TSADKGAVRD TSKLED

References

[1]

"Complete sequence of Shewanella sediminis HAW-EB3." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAW-EB3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000821 Genomic DNA. Translation: ABV38766.1.
RefSeq	YP_001475894.1. NC_009831.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A8G0Z3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5612415.
GenomeReviews	Gene locus Ssed_4162 in contig CP000821_GR.
KEGG	sse:Ssed_4162.
NMPDR	fig|425104.3.peg.3717.
PATRIC	23566256. VBISheSed62411_4423.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	MALVIRY.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	SSED425104:SSED_4162-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_SHESH

Accession

Primary (citable) accession number: A8G0Z3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program