Reviewed, UniProtKB/Swiss-Prot A8FZ49 (MAO1_SHESH)

Names and origin

Protein names

Recommended name:
    NAD-dependent malic enzyme
      Short name=NAD-ME
    EC=1.1.1.38

Gene names

Name:	sfcA
Ordered Locus Names:	Ssed_3518

Organism

Shewanella sediminis (strain HAW-EB3) [Complete proteome] [HAMAP]

Taxonomic identifier

425104 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Ligand	Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 562	562	NAD-dependent malic enzyme		PRO_1000088077
Sites
	Active site	101	1	Proton donor By similarity
	Active site	172	1	Proton acceptor By similarity
	Metal binding	243	1	Divalent metal cation By similarity
	Metal binding	244	1	Divalent metal cation By similarity
	Metal binding	267	1	Divalent metal cation By similarity
	Binding site	154	1	NAD By similarity
	Binding site	267	1	NAD By similarity
	Binding site	415	1	NAD By similarity
	Site	267	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8FZ49-1 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: 7C01247D61C6C62A	FASTA	562	62,472
	10 20 30 40 50 60 MDDHKRPLYL PFAGPAILEA PLINKGSAFT DEERVFFNLE GLLPHVIETI EEQASRAYDQ 70 80 90 100 110 120 YTNFTNDLDK HIYLRNIQDT NETLYYRLVQ NHITEMMPII YTPTVGMACE RFSKNYRRNR 130 140 150 160 170 180 GLFISYPNKD RIDDLLNNST RQKVKIIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG 190 200 210 220 230 240 GISPAYTLPI TLDVGTDNPN LLEDPMYMGW RHQRIGGEEY TEFVEAFMQA VHRRWPDALI 250 260 270 280 290 300 QFEDFAQKNA MPLLERYKDQ YCCFNDDVQG TAAVTVGSLL AACKAAKTKL SEQRVTFLGA 310 320 330 340 350 360 GSAGCGIAEA IVAQMIAEGL SEEQARAQVF MVDRWGLLQD NMPTLLPFQQ KLAQKCDDIE 370 380 390 400 410 420 GWDNFSENIS LLDVVNNAKP TVLIGVSGAP GVFSEEIIKA MHSHCPRPIV FPLSNPTSRV 430 440 450 460 470 480 EATPKDLLHW TKGQALVATG SPFEPVVVED ETFEIAQCNN SYIFPGIGLG VLAAGAKRVS 490 500 510 520 530 540 NEMLMASSRA LAECSPLALD GEGPLLPPLE EIHKVSKHIA LAVGKVAIEQ GHALPCTDEL 550 560 LEQSIEDNFW TAEYRRYKRT SF

References

[1]

"Complete sequence of Shewanella sediminis HAW-EB3." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000821 Genomic DNA. Translation: ABV38122.1.
RefSeq	YP_001475250.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5612351.
GenomeReviews	Gene locus Ssed_3518 in contig CP000821_GR.
KEGG	sse:Ssed_3518.
NMPDR	fig|425104.3.peg.3117.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01619. [Tree]
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAO1_SHESH

Accession

Primary (citable) accession number: A8FZ49

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 13, 2007
Last modified:	November 25, 2008
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)