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A8FZ10 (SYL_SHESH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Ssed_3479
OrganismShewanella sediminis (strain HAW-EB3) [Complete proteome] [HAMAP]
Taxonomic identifier425104 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074844

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif622 – 6265"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6251ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8FZ10 [UniParc].

Last modified November 13, 2007. Version 1.
Checksum: FFC1C92C73295E86

FASTA86398,073
        10         20         30         40         50         60 
MQEQYKPSEI EAKVQQHWQD KKTFEVTEDE NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVARYQRLQG KNVLQPIGWD SFGLPAENAA IKNNTAPAPW TYENIDYMKN QLKMLGFGYD 

       130        140        150        160        170        180 
WSREIATCTP EYYRWEQWFF TKLFEKGLVY KKTASVNWCP NDETVLANEQ VIDGCCWRCD 

       190        200        210        220        230        240 
TTVEQKEIPQ WFIKITEYAD ELLKDIDQLD EWPEQVKTMQ RNWIGRSEGI EMTFGVVDSE 

       250        260        270        280        290        300 
ETFDIYTTRP DTVMGVTYVA IAAGHPLAEK AAANNSELSD FIEECKNADT TEAAMAAMEK 

       310        320        330        340        350        360 
KGVATGLYAT HPLTGKQVPI WAANFVLMNY GTGAVMSVPA HDQRDYEFAT KYGLAIEGVI 

       370        380        390        400        410        420 
KPSDSDLDIS EEAYTEKGVL FNSGDSFPEL DGLDFQDAFD AIDAKLSSEG KGKRQVNFRL 

       430        440        450        460        470        480 
RDWGVSRQRY WGAPIPMVTL ADGTVVPTPE DQLPVILPED VVMDGIQSPI KADKEWAKTQ 

       490        500        510        520        530        540 
INGQEAFRET DTFDTFMESS WYYARYCSPH ADEMLDPAKA NYWLPVDQYI GGIEHACMHL 

       550        560        570        580        590        600 
LYFRFFHKLL RDIGLVNSDE PAKRLLTQGM VLADAFYYNN EKGARVWVAP SDVTVQETDE 

       610        620        630        640        650        660 
KGRIQKAVDS EGHELVYTGM SKMSKSKNNG IDPQVMVDKY GADTVRLFMM FAAPPELTLE 

       670        680        690        700        710        720 
WQESSVEGAH RFIKRLWKVA HDHVAKGATA PLDVKTLDTK QKELRRELHK TIVKVGDDIE 

       730        740        750        760        770        780 
RRQMFNTAIA SVMELMNRLQ KAPTETQQDR ALMQEALSAV VRLLYPIIPH TSFSLWNDLG 

       790        800        810        820        830        840 
NEENIEDVRW PEADQSALVE DSKLIIVQVN GKLRAKITVP ADATKEVVEE QGFAEEGVIK 

       850        860 
HTEGKTVRKV IYVPGKLLNI VAN 

« Hide

References

[1]"Complete sequence of Shewanella sediminis HAW-EB3."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Zhao J.-S., Richardson P.
Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: HAW-EB3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000821 Genomic DNA. Translation: ABV38083.1.
RefSeqYP_001475211.1. NC_009831.1.

3D structure databases

ProteinModelPortalA8FZ10.
SMRA8FZ10. Positions 228-416.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING425104.Ssed_3479.

Proteomic databases

PRIDEA8FZ10.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABV38083; ABV38083; Ssed_3479.
GeneID5613276.
KEGGsse:Ssed_3479.
PATRIC23564770. VBISheSed62411_3691.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSSED425104:GH7Q-3607-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_SHESH
AccessionPrimary (citable) accession number: A8FZ10
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: April 16, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries