A8FYW7 (RLMG_SHESH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	Ssed_3436

Organism

Shewanella sediminis (strain HAW-EB3) [Complete proteome] [HAMAP]

Taxonomic identifier

425104 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Shewanellaceae › Shewanella

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 412	412	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366515

Sequences

	Sequence		Length	Mass (Da)
	A8FYW7 [UniParc]. Last modified November 13, 2007. Version 1. Checksum: C408E98F95494BF6	FASTA	412	45,544
	10 20 30 40 50 60 MTTQFSVEGI ELELTRYPKN QESNLQAWDA ADEHLIKHLK ETEQVPVATA IINDNFGALT 70 80 90 100 110 120 ACLRSMESQW PLAVETDAKT SQLGTVQNLE NNTLSSDNIQ WLNSRENIPG AIELVLMKLP 130 140 150 160 170 180 KNLSYFAHQL NRLSQVLPEG TQVLIGAKAK SINKSLLELF AKNLGPASAS LTWKKTRVIT 190 200 210 220 230 240 CTADGKVRAL PTESQWSIPD LNLNITNLSN VFASGKLDIG ARIMLDNMPK GDFKSIIDLG 250 260 270 280 290 300 CGNGVLGLNA KQLFPQAYIH FVDDSEMAVE SARQNWALNK LDTLGLVGEQ ATFGWDDCLT 310 320 330 340 350 360 HLNEGIRPEL ILCNPPFHQG EAITDHIAWQ MFLQSWRALK NGGILHVVGN RHLAYHVKLQ 370 380 390 400 410 RIFKNCTTVA SNGKFVILQA QKISKKAEPH ENGESSSDTP NPQSSLYGGV KR

References

[1]

"Complete sequence of Shewanella sediminis HAW-EB3." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Zhao J.-S., Richardson P. Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: HAW-EB3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000821 Genomic DNA. Translation: ABV38040.1.
RefSeq	YP_001475168.1. NC_009831.1.
3D structure databases
ProteinModelPortal	A8FYW7.
ModBase	Search...
Protein-protein interaction databases
STRING	A8FYW7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5613528.
GenomeReviews	Gene locus Ssed_3436 in contig CP000821_GR.
KEGG	sse:Ssed_3436.
NMPDR	fig|425104.3.peg.3044.
PATRIC	23564668. VBISheSed62411_3648.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	NRHLGYH.
ProtClustDB	CLSK906089.
Enzyme and pathway databases
BioCyc	SSED425104:SSED_3436-MONOMER.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_SHESH

Accession

Primary (citable) accession number: A8FYW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	November 13, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program