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Protein

Lipid-A-disaccharide synthase

Gene

lpxB

Organism
Shewanella sediminis (strain HAW-EB3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate = UDP + 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl-(1->6)-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosaminyl 1-phosphate.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciSSED425104:G1G9Y-3357-MONOMER
UniPathwayiUPA00359; UER00481

Protein family/group databases

CAZyiGT19 Glycosyltransferase Family 19

Names & Taxonomyi

Protein namesi
Recommended name:
Lipid-A-disaccharide synthaseUniRule annotation (EC:2.4.1.182UniRule annotation)
Gene namesi
Name:lpxBUniRule annotation
Ordered Locus Names:Ssed_3148
OrganismiShewanella sediminis (strain HAW-EB3)
Taxonomic identifieri425104 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesShewanellaceaeShewanella
Proteomesi
  • UP000002015 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000802861 – 380Lipid-A-disaccharide synthaseAdd BLAST380

Proteomic databases

PRIDEiA8FY29

Interactioni

Protein-protein interaction databases

STRINGi425104.Ssed_3148

Structurei

3D structure databases

ProteinModelPortaliA8FY29
SMRiA8FY29
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D0V Bacteria
COG0763 LUCA
HOGENOMiHOG000018003
KOiK00748
OMAiPTVWAWR
OrthoDBiPOG091H01NW

Family and domain databases

HAMAPiMF_00392 LpxB, 1 hit
InterProiView protein in InterPro
IPR003835 Glyco_trans_19
PANTHERiPTHR30372 PTHR30372, 1 hit
PfamiView protein in Pfam
PF02684 LpxB, 1 hit
TIGRFAMsiTIGR00215 lpxB, 1 hit

Sequencei

Sequence statusi: Complete.

A8FY29-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSETTHPTFA MVAGELSGDI LGAGLIKALQ HQYPDARFVG IGGPRMEALG
60 70 80 90 100
FESIFSFEEL AVMGIVEVLS RLQRLLKVRK TLIDEICSIE PACFIGIDAP
110 120 130 140 150
DFNIGLELKL KARGIKTVHY VSPSVWAWRP KRIFKIAKAT DMVLSLLPFE
160 170 180 190 200
KAFYDKHDVP CTFVGHTLAD DIPLISDKTA ARNLLGLDAD AEYLAVLPGS
210 220 230 240 250
RGGELKQLAE PFVKAASLIK KRYPDIRFVT PLVNQKRREQ FEEALKLHAP
260 270 280 290 300
DLEITLVEGH SREVMAASDC ILLASGTATL EAMLVKRPMV VAYRVSPITY
310 320 330 340 350
KIAKGMMQID QYSLPNLLSG ETLVTELIQE NCTESLIADA ISEQLDSDFS
360 370 380
PLKEKFMQLH KGLKCNASER AAEAVIKLIQ
Length:380
Mass (Da):42,011
Last modified:November 13, 2007 - v1
Checksum:i8EE224D7DF3C63EC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000821 Genomic DNA Translation: ABV37752.1
RefSeqiWP_012143482.1, NC_009831.1

Genome annotation databases

EnsemblBacteriaiABV37752; ABV37752; Ssed_3148
KEGGisse:Ssed_3148

Similar proteinsi

Entry informationi

Entry nameiLPXB_SHESH
AccessioniPrimary (citable) accession number: A8FY29
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 13, 2007
Last modified: May 23, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health